The preparation of global distribution map of Paramecium and its endonuclear symbiotic bacterium Holospora

草履虫及其核内共生菌全孢菌全球分布图的绘制

• 批准号: 13575007
• 负责人: FUJISHIMA Masahiro
• 金额: $ 4.99万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575007/
• 关键词: Holospora; paramecium; Geographic distribution; Endonuclear symbiotic bacterium; Stress resistance; Cell suraface proteins; 繊毛虫; 分布; 野外採集; 共生; 世界分布図

(1) A new Parameciuim species was found from water samples collected from Shanghai, China, and the species was named as Paramecium schewiakoffi (in press)(2) For the first time, we collected P. decaurelia and P. dodecaurelia in Japan (Pryzybos et al., Folia boil., 51, 223-224, 2003)(3) A new Holospora species was found in the macronucleus of P.bursaria collected in Morioka city, and the bacterium was named as Holospora hiwatashii (in preparation)(4) N.obtusa bearing P.caudatum was collected in Yamaguchi city. This is the most Southern place where Holospora species had ever collected(5) Collection records on Holospora species by H.-D.Gortz, S.I.Fokin and M.Fujishima for the past 20 years were collected and edited(6) We found that infection of Holospora transforms the host cells to various sress-resistant, and clarified molecular mechanism of this phenomenon (Hori and Fujishima, J.Euk. Microbiol., 50, 293-298, 2003 ; Nakamura et al., in preparation)(7) We found 20 host genes, of which expressions were altered by infection of H.obtusa by the differential display method, and four genes were cloned (Nakamura et al., in preparation ; Aikawa et al, in preparation)

(1)从中国上海采集的水样中发现草履虫属一新种，命名为Schewiakoffi草履虫（出版中）（2）首次在日本采集到十尾草履虫和十二尾草履虫（Pryzybos et al.，叶状体煮沸，51，223-224，2003）（3）在收集于盛冈市的绿伞（P.bursaria）的大核中发现了一个新的全孢子菌属（Holospora）种，并将该细菌命名为Holospora hiwatashii（in preparation）（4）在山口市收集了带有尾状绿伞的钝圆孢菌（N.obtusa）。这是迄今为止收集到的最南的全孢霉种的地方。（5）H.-（6）我们发现全孢菌感染可使宿主细胞转化为各种抗胁迫的细胞，并阐明了这种现象的分子机制（Hori和Fujishima，J.Euk.微生物，50，293-298，2003 ;中村等人，（7）我们发现了20个宿主基因，通过差异显示方法，其中的表达被钝头血吸虫感染而改变，并且克隆了4个基因（中村等人，在准备中; Aikawa等人，在准备中）

项目成果

藤島政博: "生物学入門(大学生のための基礎シリーズ2)(石川統編)(川口明彦, 藤島政博, 大森正之, 石川 統, 西駕秀俊, 塚谷裕一, 上村慎治, 和田 勝, 嶋田正和 著)"(株)東京化学同人. 283 (2001)

藤岛正宏：《生物学概论（大学生基础系列2）（石川修主编）（川口明彦、藤岛正宏、大森正幸、石川修、西利秀俊、冢谷佑一、植村新司、和田胜、岛田正一） 《东京化学同人社》283（2001）

S.I.Fokin, M.Schweikert, H.-D.Gonz, M.Fujishima: "Bacterial endocytobionts of Ciliophora. Diversity and some interactions with the host"European Journal of Protistology. 39. 475-480 (2003)

S.I.Fokin、M.Schweikert、H.-D.Gonz、M.Fujishima：“纤毛虫的细菌内吞生物。多样性以及与宿主的一些相互作用”欧洲原生学杂志。

I.N.Skovorodkin, S.I.Fokin, M.Fujishima: "Fates of the endonuclear symbiotic bacteria Holospora obtusa and Holospora undulata infected into the macronucleus of Paramecium caudatum"European Journal of Protistology. 237. 137-145 (2001)

I.N.Skovorodkin、S.I.Fokin、M.Fujishima：“感染尾草履虫大核的核内共生细菌 Holospora obtusa 和 Holospora undulata 的命运”欧洲原生学杂志。

藤島政博: "第2章 細胞.生物学入門(大学生のための基礎シリーズ2)"東京化学同人. 283 (2001)

藤岛正宏：“第2章细胞生物学概论（大学生基础系列2）”东京化学同人283（2001）。

M.Sugii, M.Fujishima: "Purification of GVBD-inducing protein from the ciliate Tetrahymena thermophila."Journal of Eukaryotic Microbiology. 48. 414-424 (2001)

M.Sugii，M.Fujishima：“从纤毛虫嗜热四膜虫中纯化 GVBD 诱导蛋白。”真核微生物学杂志。