Polymorphism in cancer-related genes among oral cancers from the eastern Asian area

东亚地区口腔癌癌症相关基因多态性

• 批准号: 13576025
• 负责人: CHENG Jun
• 金额: $ 8.64万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576025/
• 关键词: Asia nation; oral cancer; superficial squamous cell carcinoma; pre-cancer; dysplasia; orthoprosthesis; chew tobacco; pathologic epidemiology; アポトーシス; 噛みたばこ; 口腔癌; 癌関連遺伝子; リンパ球; 表在癌; マイクロディセクション; PCR; 東アジア; 南アジア

Oral cancers and precancerous lesions were collected from China, Sri-Lanka, Bangladesh, and India. Among the present collection of oral cancers and precancerous lesions, we found lesions which can be referred to as superficial carcinoma in many areas with or without the custom of chewing tobacco. There were 128 cases of superficial carcinoma among 800 cases of oral squamous cell carcinoma. Among them, 82 superficial carcinomas out of 680 cases from the Japanese population. The clinicopathobgical characteristics of the Japanese patients with superficial carcinoma were as follows : the patients were mainly older women ; the superficial carcinoma arose more commonly in the longue, gingival, and buccal mucosa ; the Lesions were multiple and recurrent ; less consumptions of tobacco for smoking and alcohol than patients with invasive carcinomas ; the superficial carcinoma lesions had some association with the patients' anamneses for prosthetic treatments. In contrast, the lesion from Asian c … More ountries was found exclusively in the buccal mucosa and gingival, and the patients were mainly males with chewing habits. Histopathobgy of the superficial carcinoma was the same among the Japanese and Asian patients. They were commonly composed of carcinoma in-situ, which could be divided into three types, such as basaloid, verrucous, and acanthotic, and of surrounding epithelial dysplasia with the characteristic two-phase appearances. In order to recognize such a disease entity of superficial carcinoma of the oral mucosa, a workshop was taken place in Singapore in August 2002, at which oral pathologists from Japan, Korea, Malaysia, Indonesia, Sri-Lanka, and the United Kingdom attended and discussed about the possibility of the conceptUsing paraffin-embedded specimens collected from Asian countries, superficial carcinomas were investigated in several aspects as follows : the characteristic two-phase appearance in epithelial dysplasia was investigated by immunohistochemistry and in-situ hybridization, and its molecular mechanism was revealed to be due to apoptosis of prickle cells by mechanical stress of basaloid cell proliferation in the lower layer and thick keratinization in the surface layer. In addition, extracellular matrix remodeling in oral submucous fibrosis was determined for one of the backgrounds of oral carcinogenesis. DNA samples obtained by microdissection were analyzed for mutational events in cancer-related genes. Among them, there were several characteristic mutations in the p53 gene which were shared by the collected cases.Based on the data obtained in this study, it is highly suggested that superficial carcinomas of the oral mucosa caused by different environmental factors shared similar genetic alterations in the cancer-related genes. Less

口腔癌和癌前病变收集自中国、斯里兰卡、孟加拉国和印度。在目前收集的口腔癌和癌前病变中，我们在许多有或没有咀嚼烟草习惯的地区发现了可称为浅表癌的病变。 800例口腔鳞癌中，浅表癌128例。其中，680 例日本人群中有 82 例为浅表癌。日本浅表性癌患者的临床病理特征如下：以老年女性为主；浅表癌多见于长牙、牙龈、颊粘膜；病变多发且反复发作；与浸润性癌患者相比，吸烟和饮酒的烟草消耗量较少；浅表癌病变与患者的修复治疗史有一定的相关性。相比之下，亚洲国家的病变仅发生在颊粘膜和牙龈，且患者以有咀嚼习惯的男性为主。日本和亚洲患者浅表癌的组织病理学相同。它们通常由原位癌组成，可分为基底样癌、疣状癌和棘皮样癌三种类型，周围上皮不典型增生，具有特征性的两相性外观。为了认识口腔粘膜浅表癌这一疾病实体，2002年8月在新加坡举办了一次研讨会，来自日本、韩国、马来西亚、印度尼西亚、斯里兰卡和英国的口腔病理学家参加了研讨会，并讨论了这一概念的可能性。使用从亚洲国家收集的石蜡包埋标本，对几个国家的浅表癌进行了研究。 通过免疫组化和原位杂交研究了上皮不典型增生的特征性两相表现，揭示其分子机制是由于下层基底细胞增殖和表层角质化厚层的机械应力导致棘细胞凋亡。此外，口腔粘膜下纤维化中的细胞外基质重塑被确定为口腔癌发生的背景之一。分析通过显微切割获得的 DNA 样本中癌症相关基因的突变事件。其中，p53基因存在多个特征性突变，这些突变是收集病例所共有的。根据本研究获得的数据，强烈提示由不同环境因素引起的口腔粘膜浅表癌在癌症相关基因中具有相似的遗传改变。较少的

项目成果

Superficial carcinoma of the oral mucosa : a new disease entity

口腔粘膜浅表癌：一种新的疾病实体

• 发表时间: 2002
• 期刊: Niigata Dental Journal 32(1)
• 作者: Azuma R;Syafriadi M;Suzuki M;Ida H;Cheng J;Saku T
• 通讯作者: Saku T

Cheng J: "Clinicaopathological characteristics of superficial carcinoma of the oral mucosa : prosthodontic anamneses as its pathogenetic factor"Cancer Science. 94(S). 459 (2003)

程杰：“浅表性口腔粘膜癌的临床病理特征：修复病史作为其致病因素”《癌症科学》。

口腔粘膜表在癌:新しい疾患概念

口腔粘膜浅表癌：一个新的疾病概念

• 发表时间: 2002
• 期刊: Niigata Dent J 32(1)
• 作者: 東 良平

Syafriadi M: "Carcinoma in-situ of the oral mucosa has a definite tendency towards keratinization"Oral Med Pathol. 8(2). 43-44 (2003)

Syafriadi M：“口腔粘膜原位癌具有明确的角化倾向”Oral Med Pathol。

Extracellular matrix remodeling in oral submucous fibrosis:: its stage-specific modes revealed by immunohistochemistry and in situ hybridization

• DOI: 10.1111/j.1600-0714.2005.00339.x
• 发表时间: 2005-09-01
• 期刊: JOURNAL OF ORAL PATHOLOGY & MEDICINE
• 影响因子: 3.3
• 作者: Utsunomiya, H;Tilakaratne, WM;Saku, T
• 通讯作者: Saku, T