Molecular mechanisms of ghost cell formation and calcification in calcifying cystic odontogenic tumor (CCOT) cell lines

钙化囊性牙源性肿瘤（CCOT）细胞系中鬼细胞形成和钙化的分子机制

• 批准号: 22592033
• 负责人: CHENG Jun
• 金额: $ 2.83万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592033/
• 关键词: 歯原性腫瘍; 石灰化歯原性嚢胞; 幻影細胞; 石灰化; 細胞外基質; 細胞培養; 脱核; 免疫組織化学; RT-PCR

Calcifying cystic odontogenic tumor (CCOT) is histopathologically characterized by the emergence of ghost cells. However, their histopathogenesis has been poorly understood. To understand the cellular mechanism towards ghost cell formation, we have successfully established of cell lines from a CCOT surgical specimen. Using these CCOT cells, we successfully demonstrated that ghost cells were generated due to intracellular deposits of extracellular matrix molecules including perlecan, and that their calcification was started in those matrices and completed by proteolysis of deposited matrices towards their denucleated forms.

钙化囊性牙源性肿瘤（CCOT）的组织病理学特征是鬼影细胞的出现。然而，对其组织发病机制了解甚少。为了了解鬼细胞形成的细胞机制，我们成功地从CCOT手术标本中建立了细胞系。利用这些CCOT细胞，我们成功地证明了鬼细胞是由于细胞外基质分子（包括perlecan）在细胞内沉积而产生的，并且它们的钙化始于这些基质，并通过沉积基质的蛋白质水解而完成。

项目成果

Intramuscular keratocyst as a soft tissue counterpart of keratocystic odontogenic tumor: differential diagnosis by immunohistochemistry.

肌内角化囊肿作为角化囊性牙源性肿瘤的软组织对应物：免疫组织化学鉴别诊断。

• DOI: 10.1016/j.humpath.2013.08.011
• 发表时间: 2013
• 期刊: Hum Pathol.
• 作者: Abe T;Maruyama S;Yamazaki M;Essa A;Babkair H;Mikami T;Shingaki S;Kobayashi T;Hayashi T;Cheng J;Saku T.
• 通讯作者: Saku T.

側頭筋-頬筋内嚢胞性病変

颞颊肌内囊性病变

• 发表时间: 2012
• 作者: Cheng J;Wu L;Maruyama S;Yamazaki M;Saku T;Cheng J;阿部 達也
• 通讯作者: 阿部 達也

Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions: its practical use in surgical pathology

• DOI: 10.1111/j.1365-2559.2010.03712.x
• 发表时间: 2010-12
• 期刊: Histopathology
• 影响因子: 6.4
• 作者: M. Tsuneki;M. Yamazaki;Jun Cheng;S. Maruyama;Takanori Kobayashi;T. Saku

Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma

口腔鳞状细胞癌侵袭时细胞外基质产生的实质-基质转换

• DOI: 10.1016/j.humpath.2012.02.006
• 发表时间: 2012
• 期刊: Human Pathology
• 影响因子: 3.3
• 作者: Metwaly H;Maruyama S;Yamazaki M;Tsuneki M;Abe T;Jen KY;Cheng J;Saku T.
• 通讯作者: Saku T.

Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions : its practical in surgical pathology

联合免疫组织化学鉴别诊断颌骨囊性病变：在外科病理学中的应用

• DOI: 10.1111/j.1365-2559.2010.03721.x
• 发表时间: 2010
• 期刊: Histopathology
• 影响因子: 6.4
• 作者: Tsuneki M;Yamazaki M;Cheng J;Maruyama S;Kobayashi T;Saku T
• 通讯作者: Saku T