OPTIMIZATION OF NANOFLOW LC FOR IMPROVED PEAK CAPACITY AND PEPTIDE IDS

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 在鸟枪法蛋白质组学中，反相纳流 HPLC 被广泛用作在质谱分析之前分离肽的方法。肽的色谱分离可以对复杂的肽样品进行分析，而这在其他情况下是不可能的。我们感兴趣的是改变 nanoLC 参数（例如柱长和梯度长度）对峰容量和肽鉴定的影响。我们还对获得最佳色谱峰容量是否会导致最佳肽鉴定感兴趣。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In shotgun proteomics, reverse phase nanoflow HPLC is widely used as a method of separating peptides prior to analysis by mass spectrometry. The chromatographic separation of peptides enables the analysis of complex peptide samples that would otherwise not be possible. We are interested in the effect of altering nanoLC parameters, such as column length and gradient length, on peak capacity and peptide identifications. We are also interested in whether obtaining optimal chromatographic peak capacity results in optimal peptide identifications.