OPTIMIZATION OF NANOFLOW LC FOR IMPROVED PEAK CAPACITY AND PEPTIDE IDS

优化纳流液相色谱以提高峰容量和肽 ID

• 批准号: 8365816
• 负责人: Michael MacCoss
• 金额: $ 0.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365816
• 关键词: Biology; Complex; Funding; Fungal Genome; Grant; High Pressure Liquid Chromatography; Length; Mass Spectrum Analysis; Methods; National Center for Research Resources; Peptides; Phase; Principal Investigator; Proteomics; Research; Research Infrastructure; Resources; Sampling; Shotguns; Source; United States National Institutes of Health; cost; improved; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In shotgun proteomics, reverse phase nanoflow HPLC is widely used as a method of separating peptides prior to analysis by mass spectrometry. The chromatographic separation of peptides enables the analysis of complex peptide samples that would otherwise not be possible. We are interested in the effect of altering nanoLC parameters, such as column length and gradient length, on peak capacity and peptide identifications. We are also interested in whether obtaining optimal chromatographic peak capacity results in optimal peptide identifications.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在鸟枪法蛋白质组学中，反相纳米流HPLC被广泛用作在通过质谱分析之前分离肽的方法。肽的色谱分离使得能够分析复杂的肽样品，否则这是不可能的。我们感兴趣的是改变nanoLC参数，如柱长和梯度长度，对峰容量和肽鉴定的影响。我们还感兴趣的是，是否获得最佳的色谱峰容量的结果在最佳的肽鉴定。