Compulsory ordination of biological cells in wave-guide long-path optical cell for detection of trace components in biological cells.

波导长光程光学单元中生物细胞的强制排序，用于检测生物细胞中的痕量成分。

基本信息

批准号：
14340235
负责人：
FUJIWARA Kitao
金额：
$ 10.62万
依托单位：
Tokyo University of Pharmacy and Life Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

1.Teflon AF 2400 was recently commercialized, that has a refractive index(1.29) lower than water (1.33), and which means that the wave-guiding of light is possible in water. We used Teflon AF 2400 as a wave-guide capillary longpath cell for fluorometry. He-Cd and Ar lasers were used as the excitation source, at 325 nm and 514.5 nm, respectively. The excitation was executed through the wave-guide cell and the fluorescence from the wound capillary cell was collected in a perpendicular direction to the loop. With excitation at 325 nm, the fluorescence intensity at 450 nm emitted from the cell wall decreased along with the increase in the refractive index of the solvent. This can be caused by attenuation of the source light due to absorption by the solvent. In our experiment, the solvent of the higher refractive index has the higher absorption at 325 nm. On the other hand, the fluorescence intensity at 590 nm, with excitation at 514.5 nm, increases with refractive index of the solvent.2.Th … More e wave-guide cell was used for irradiation of solar light in the solution. In connection with the photo-chemical processes in the aquatic environment, the evaluation system for the production ability of superoxide anion was constructed. The technique was based on the flow injection method with luminol chemiluminescence detection. After irradiation was finished, the carrier (with sample) flow was merged with the luminol solution and was introduced into a chemiluminescence detector. The results of the laboratory experiment show that the production of superoxide is linearly related to the concentration of humic acid up to 50 ppm. By means of changing the flow rate of the carrier, the half-life time of superoxide at pH 11 aqueous solution was estimated as 15 s. ESR spectra were obtained after 5 min. irradiation of the solar simulator. In addition to the four sharp peaks due to OH radicals, a broad peak appeared at the middle of the OH signal. The river water was collected at 18 points of the Tamagawa River located in Tokyo. Upstream, the production ability of superoxide was observed, but not downstream or in the estuarine district.3.When heavy metal ions such as Zn(II), Ni(II) and PT(IV), Chlorella kessleri (minute unicellular algae) transformed to a large connected cell (not-devided). The regular type and the large connected type cell was introduced into the wave-guide cell mentioned in 1. The auto-correlation function was taken from the fluorescence of the wave-guide capillary, and some difference based on the cell shape was obtained. Less

1.TEFLON AF 2400最近被商业化，其折射率（1.29）低于水（1.33），这意味着在水中可能会引导光的波浪。我们将Teflon AF 2400用作波型毛细管长路径细胞进行荧光测定法。 HE-CD和AR激光在325 nm和514.5 nm处用作兴奋源。兴奋是通过波引物细胞执行的，并沿垂直于环的垂直方向收集了从伤口毛细管细胞的荧光。在325 nm处的兴奋时，从细胞壁发出的450 nm处的荧光强度随着溶解度的折射率的增加而降低。这可能是由于偿付能力遭受痛苦而导致的源光的衰减。在我们的实验中，较高的折射率解决方案在325 nm处的损失较高。另一方面，在590 nm处的荧光强度在514.5 nm处兴奋，随着溶液的折射率而增加。2。Tht…使用溶液中更多的E波螺旋细胞来辐射太阳光。与水生环境中的光化过程有关，构建了超氧化阴离子生产能力的评估系统。该技术基于使用Luminol化学发光检测的流量注射方法。辐照完成后，载体（带有样品）流动的实验结果表明，超氧化物的产生与高达50 ppm的腐殖酸的浓度线性相关。通过更改载体的流速，pH 11水溶液时超氧化物的半衰期时间估计为15 s。 5分钟后获得ESR光谱。太阳能模拟器的辐照。除了OH自由基引起的四个尖峰之外，OH信号中间出现了一个宽峰。河水是在东京塔川河的18点收集的。上游，观察到超氧化物的生产能力，但在下游或河口区中没有观察到。3。重金属离子（例如Zn（ii），Ni（ii）和Pt（IV）等重金属离子时，Chlorella Kessleri（分钟单细胞藻类）转化为大型连接细胞（未脱落）。常规类型和大型连接类型的细胞被引入1中提到的波为波为波形细胞中。自动相关函数是从波螺旋毛细管的荧光中获取的，并获得了基于细胞形状的一些差异。较少的

项目成果

期刊论文数量（63）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Evaluation of in vivo immunotoxicity of a major organic arsenic compound arsenobetaine in seafood.

海鲜中主要有机砷化合物砷甜菜碱的体内免疫毒性评价。

DOI：
发表时间：
2004
期刊：
Int.Immupharmac. 4(2)
影响因子：
0
作者：
Sakurai;T.;Kojima;C.;Ochiai.M.;Ohta;T.;Fujiwara;K.
通讯作者：
K.

Effects of arsenobetain, a major organic arsenic compound in Seafood.

砷甜菜碱（海鲜中的一种主要有机砷化合物）的作用。

DOI：
发表时间：
2004
期刊：
Appl.Organomet.Chem. 18
影响因子：
0
作者：
Toyoda;R.;Kasai;A.;Sato;S.;Wada;S.;Saiga;H.;Ikeo;K.;Gojobori;T.;Numakunai;T.;Yamamoto;H.;Takami Ohta 他
通讯作者：
Takami Ohta 他

中村洋, 藤原祺多夫, 他編: "分析試料の前処理ハンドブック"丸善. 1053 (2003)

Hiroshi Nakamura、Keitao Fujiwara 等编：“分析样品预处理手册”Maruzen 1053 (2003)。