权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Compulsory ordination of biological cells in wave-guide long-path optical cell for detection of trace components in biological cells.

波导长光程光学单元中生物细胞的强制排序，用于检测生物细胞中的痕量成分。

基本信息

批准号：
14340235
负责人：
FUJIWARA Kitao
金额：
$ 10.62万
依托单位：
Tokyo University of Pharmacy and Life Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

1.Teflon AF 2400 was recently commercialized, that has a refractive index(1.29) lower than water (1.33), and which means that the wave-guiding of light is possible in water. We used Teflon AF 2400 as a wave-guide capillary longpath cell for fluorometry. He-Cd and Ar lasers were used as the excitation source, at 325 nm and 514.5 nm, respectively. The excitation was executed through the wave-guide cell and the fluorescence from the wound capillary cell was collected in a perpendicular direction to the loop. With excitation at 325 nm, the fluorescence intensity at 450 nm emitted from the cell wall decreased along with the increase in the refractive index of the solvent. This can be caused by attenuation of the source light due to absorption by the solvent. In our experiment, the solvent of the higher refractive index has the higher absorption at 325 nm. On the other hand, the fluorescence intensity at 590 nm, with excitation at 514.5 nm, increases with refractive index of the solvent.2.Th … More e wave-guide cell was used for irradiation of solar light in the solution. In connection with the photo-chemical processes in the aquatic environment, the evaluation system for the production ability of superoxide anion was constructed. The technique was based on the flow injection method with luminol chemiluminescence detection. After irradiation was finished, the carrier (with sample) flow was merged with the luminol solution and was introduced into a chemiluminescence detector. The results of the laboratory experiment show that the production of superoxide is linearly related to the concentration of humic acid up to 50 ppm. By means of changing the flow rate of the carrier, the half-life time of superoxide at pH 11 aqueous solution was estimated as 15 s. ESR spectra were obtained after 5 min. irradiation of the solar simulator. In addition to the four sharp peaks due to OH radicals, a broad peak appeared at the middle of the OH signal. The river water was collected at 18 points of the Tamagawa River located in Tokyo. Upstream, the production ability of superoxide was observed, but not downstream or in the estuarine district.3.When heavy metal ions such as Zn(II), Ni(II) and PT(IV), Chlorella kessleri (minute unicellular algae) transformed to a large connected cell (not-devided). The regular type and the large connected type cell was introduced into the wave-guide cell mentioned in 1. The auto-correlation function was taken from the fluorescence of the wave-guide capillary, and some difference based on the cell shape was obtained. Less

1.Teflon AF2400最近商业化了，它的折射率(1.29)比水(1.33)低，这意味着光在水中的波导性是可能的。采用聚四氟乙烯AF2400作为光导毛细管长光程池进行荧光检测。用He-Cd和Ar激光作为激发源，波长分别为325 nm和514.5 nm。通过波导池进行激发，并在垂直于环路的方向收集来自伤口毛细管池的荧光。当激发波长为325 nm时，从细胞壁发射的450 nm荧光强度随着溶剂折射率的增加而减小。这可能是由于被溶剂吸收而导致的光源光衰减所致。在我们的实验中，折射率越高的溶剂在325 nm处有较高的吸收。另一方面，当激发波长为514.5 nm时，590 nm处的荧光强度随着溶液的折射率的增加而增加。2.…溶液中太阳光的照射采用了更多的e波导池。针对水环境中的光化学过程，构建了超氧阴离子产生能力的评价体系。该技术基于鲁米诺化学发光检测的流动注射方法。辐照结束后，将载流(带样品)与鲁米诺溶液合并，并将其引入化学发光检测器。实验室实验结果表明，当腐植酸浓度达到50ppm时，超氧化物的产生与腐植酸的浓度呈线性关系。通过改变载体的流速，测得超氧化物在pH为11的水溶液中的半衰期为15 S，5min后得到电子自旋共振波谱。太阳模拟器的辐照。除了四个由OH自由基引起的尖锐峰外，在OH信号的中间还出现了一个宽峰。河水是在位于东京的玉马川河的18个点收集的。上游有超氧化物的产生能力，但下游和河口区没有。3.当重金属离子锌(II)、镍(II)和铂(IV)转化为大的连通细胞(不分离)时，小球藻(小球藻)(微小的单细胞藻类)转化为一个大的连通细胞。在文[1]中提出的光导池中引入了规则型和大连通型光电池，从光导毛细管的荧光中提取自相关函数，得到了不同形状的光波导池。较少

项目成果

期刊论文数量（63）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

中村洋, 藤原祺多夫, 他編: "分析試料の前処理ハンドブック"丸善. 1053 (2003)

Hiroshi Nakamura、Keitao Fujiwara 等编：“分析样品预处理手册”Maruzen 1053 (2003)。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Evaluation of in vivo immunotoxicity of a major organic arsenic compound arsenobetaine in seafood.

海鲜中主要有机砷化合物砷甜菜碱的体内免疫毒性评价。

DOI：
发表时间：
2004
期刊：
Int.Immupharmac. 4(2)
影响因子：
0
作者：
Sakurai;T.;Kojima;C.;Ochiai.M.;Ohta;T.;Fujiwara;K.
通讯作者：
K.

Effects of arsenobetain, a major organic arsenic compound in Seafood.

砷甜菜碱（海鲜中的一种主要有机砷化合物）的作用。

DOI：
发表时间：
2004
期刊：
Appl.Organomet.Chem. 18
影响因子：
0
作者：
Toyoda;R.;Kasai;A.;Sato;S.;Wada;S.;Saiga;H.;Ikeo;K.;Gojobori;T.;Numakunai;T.;Yamamoto;H.;Takami Ohta 他
通讯作者：
Takami Ohta 他

Teruaki Sakiral: "Cellular glutathione prevents cytolethslity of - monomethylarsonic acid."Toxicol.Appl.Pharmacol.. in press.

Teruaki Sakiral：“细胞谷胱甘肽可防止单甲基胂酸的细胞杀伤力。”Toxicol.Appl.Pharmacol.. 正在出版。