Compulsory ordination of biological cells in wave-guide long-path optical cell for detection of trace components in biological cells.

1.折射率（1.29）比水（1.33）低的Teflon AF 2400最近实现了商用化，这意味着可以在水中引导光波。我们使用特氟龙af2400作为波导毛细管长径细胞进行荧光测定。He-Cd和Ar激光器分别在325 nm和514.5 nm处作为激发源。通过波导细胞进行激发，从缠绕的毛细管细胞中收集与环路垂直方向的荧光。在325 nm激发下，细胞壁在450 nm处发出的荧光强度随着溶剂折射率的增加而降低。这可能是由于溶剂的吸收导致光源的衰减造成的。在我们的实验中，高折射率的溶剂在325 nm处有较高的吸收。另一方面，当激发波长为514.5 nm时，590nm处的荧光强度随溶剂折射率的增加而增加。采用波导电池对溶液中的太阳光进行辐照。结合水体环境中的光化学过程，构建了超氧阴离子生成能力评价体系。该技术是基于鲁米诺化学发光检测的流动注射法。辐照结束后，载体（带样品）流与鲁米诺溶液合并，引入化学发光检测器。室内实验结果表明，在50 ppm以下，腐植酸的浓度与超氧化物的产量呈线性相关。通过改变载体的流速，估计超氧化物在pH为11的水溶液中的半衰期为15 s。在太阳模拟器照射5min后获得ESR光谱。除了OH自由基引起的四个尖峰外，在OH信号的中间出现了一个宽峰。在东京的玉川河的18个点收集了河水。上游观察到超氧化物的产生能力，但下游和河口地区没有。当Zn（II）、Ni（II）、PT（IV）等重金属离子存在时，kessleri小球藻（微小单细胞藻类）转化为一个大的连接细胞（未分裂）。在1中提到的波导电池中引入了规则型和大连通型电池。从波导毛细管的荧光中提取自相关函数，并根据细胞形状得到了一定的差异。少

1.Teflon AF 2400 was recently commercialized, that has a refractive index(1.29) lower than water (1.33), and which means that the wave-guiding of light is possible in water. We used Teflon AF 2400 as a wave-guide capillary longpath cell for fluorometry. He-Cd and Ar lasers were used as the excitation source, at 325 nm and 514.5 nm, respectively. The excitation was executed through the wave-guide cell and the fluorescence from the wound capillary cell was collected in a perpendicular direction to the loop. With excitation at 325 nm, the fluorescence intensity at 450 nm emitted from the cell wall decreased along with the increase in the refractive index of the solvent. This can be caused by attenuation of the source light due to absorption by the solvent. In our experiment, the solvent of the higher refractive index has the higher absorption at 325 nm. On the other hand, the fluorescence intensity at 590 nm, with excitation at 514.5 nm, increases with refractive index of the solvent.2.Th … More e wave-guide cell was used for irradiation of solar light in the solution. In connection with the photo-chemical processes in the aquatic environment, the evaluation system for the production ability of superoxide anion was constructed. The technique was based on the flow injection method with luminol chemiluminescence detection. After irradiation was finished, the carrier (with sample) flow was merged with the luminol solution and was introduced into a chemiluminescence detector. The results of the laboratory experiment show that the production of superoxide is linearly related to the concentration of humic acid up to 50 ppm. By means of changing the flow rate of the carrier, the half-life time of superoxide at pH 11 aqueous solution was estimated as 15 s. ESR spectra were obtained after 5 min. irradiation of the solar simulator. In addition to the four sharp peaks due to OH radicals, a broad peak appeared at the middle of the OH signal. The river water was collected at 18 points of the Tamagawa River located in Tokyo. Upstream, the production ability of superoxide was observed, but not downstream or in the estuarine district.3.When heavy metal ions such as Zn(II), Ni(II) and PT(IV), Chlorella kessleri (minute unicellular algae) transformed to a large connected cell (not-devided). The regular type and the large connected type cell was introduced into the wave-guide cell mentioned in 1. The auto-correlation function was taken from the fluorescence of the wave-guide capillary, and some difference based on the cell shape was obtained. Less