Development for the method of minute plant cell analysis by fluorescence. correlation spectroscopy.

荧光相关光谱(FCS)是一种测定荧光粒子大小的新技术。在FCS信号对荧光粒子直径变化的响应中，考察了针孔直径和激光光束大小等光学参数。在普通系统中，采用共焦光学结构。然而，事实证明，采用普通荧光光谱光学构型的FCS系统可以测定100 nm以下的LASS。该系统具有普通的光学荧光构型，可以成功地测定微藻。

Fluorescence correlation spectrometry (FCS) is the novel technique which can determine the size of fluorescent particles. The optical parameters such as diameter of pin-hole aperture and laser beam size were inspected in the response of FCS signal against the variation in the diameters of fluorescent particles. In the ordinary system, con-focal optical configuration was adopted. However, it has been proved that the FCS system with the ordinary optical configuration of fluorescence spectroscopy can determine the size lass than 100 nm. The present system with the ordinary optical fluorescence configuration can successfully determine the mico-algae.