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Enhancement sensitivity of smectrometry using waveguiding effect and its application to natural water analysis.

利用波导效应增强光度测量的灵敏度及其在天然水分析中的应用。

基本信息

批准号：
02453033
负责人：
FUJIWARA Kitao
金额：
$ 3.97万
依托单位：
Hroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Liquid-core optical fibers have been investigated by means of injecting the solution possessing high refractive index into capillaries made of pyrex or quartz. We have already testified the enhancement of absorbance in the solution more than 3 x 10^4 by the use of a liquid-core wave-guiding cell of which length was 50 m. In this study, application of the wave-guiding cells to fluorometry and turbidimetry was mainly studied.The optical configuration, where excitation of fluorophore in the solution was carried out from one terminal and fluorescence was collected at another terminal of the capillary cell, was first considered. In this method, the maximum fluorescence intensity in its magnitude was observed when the capillary of 60 cm was used, and extension of the cell length longer than 60 cm made decrease of fluorescence. However, the best signal to noise ratio was given by the use of a 12-m cell in which the noise contributed by the excitation source (laser) was filtrated. Another fluorometric method was studied where the source light was guided through the capillary cell and fluorescence was collected from the rectangular side of the capillary. A vortex cell is effective to use in this manner of fluorescence detection, which show the highest response to the change of refractive index of solution. An automated flow injection system has been constructed with the use of this vortex cell, which provides 2 ppb of lead as the detection limit and was successfully applied to the environmental samples.Strong magneto-optical birefringence appeared when the capillary cell was filled with the diluted magnetite colloid solution under the magnetic field. It has been attested that this birefringence is proportional to the cell length and the concentration of the magnetite colloid.

通过将具有高折射率的溶液注入到由耐热玻璃或石英制成的毛细管中，研究了液芯光纤。我们已经证明，使用长度为50 m的液芯导波池，溶液中的吸光度提高了3 × 10^4以上。本研究主要研究了导波池在荧光测定和浊度测定中的应用。首先考虑的是光学结构，即溶液中的荧光团从一端激发，而在毛细管细胞的另一端收集荧光。在本方法中，当毛细管长度为60 cm时，荧光强度达到最大，当细胞长度超过60 cm时，荧光减弱。然而，最好的信噪比是使用12米的电池，其中由激发源（激光）产生的噪声被过滤。研究了另一种荧光测定方法，将光源引导穿过毛细管细胞，从毛细管的矩形侧收集荧光。涡流池是一种有效的荧光检测方法，它对溶液折射率变化的响应最高。利用该涡流池构建了一个自动流动注射系统，该系统可提供2 ppb的铅作为检测限，并成功应用于环境样品。在磁场作用下，将稀释后的磁铁矿胶体溶液充入毛细管细胞，出现了强磁光双折射现象。已经证明，这种双折射与细胞长度和磁铁矿胶体的浓度成正比。