Autotrophic carbon dioxide fixation reaction within non-Calvin type pathway

非卡尔文型途径内的自养二氧化碳固定反应

• 批准号: 14360049
• 负责人: ISHII Masaharu
• 金额: $ 10.43万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360049/
• 关键词: Reductive TCA cycle; 3-Hydroxynronionate cycle; Hydrogenobacter thermophils; Acidionus brierleyi; Hydrogenobacter; 3-ヒドロキシプロビオン酸サイクル; Acidianus; Hydrogenobactor thermophilus

I succeeded in demonstrating in vitro carbon dioxide fixation reactions of both Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase, which are operative within Hydrogenobactei-thermophilus as key enzymes of reductive TCA cycle. Both enzymes are reversible. Therefore, during the experiment of one enzyme, the other enzyme was used for the regeneration of reduced ferredoxin. Also, isocitrate dehydrogenase reaction was analyzed in detail, biotinylated protein was shown to be essential for the carbon dioxide fixation reaction.Acyl-CoA carboxylase was also purified and characterized from a thermophilic sulfur oxidizing archeon, Acidianus brierieyi, which was a first example from archaea. The purified enzyme shared common characteristics with another acetyl-CoA carboxylases. Still, it had several characteristic differences from another acetyl-CoA carboxylases.

我成功地演示了丙酮酸：铁氧还蛋白氧化还原酶和 2-氧戊二酸：铁氧还蛋白氧化还原酶的体外二氧化碳固定反应，它们在嗜热氢杆菌中作为还原 TCA 循环的关键酶发挥作用。两种酶都是可逆的。因此，在一种酶的实验过程中，另一种酶被用于还原铁氧还蛋白的再生。此外，还详细分析了异柠檬酸脱氢酶反应，表明生物素化蛋白质对于二氧化碳固定反应至关重要。还从嗜热硫氧化古细菌 Acidianus brierieyi 中纯化并鉴定了酰基辅酶 A 羧化酶，这是古细菌的第一个例子。纯化的酶与另一种乙酰辅酶A羧化酶具有共同的特征。尽管如此，它与其他乙酰辅酶A羧化酶仍有一些特征差异。

项目成果

Masaharu Ishii et al.: "Occurrence, biochemistry and possible biotechnological application of the 3-Hydroxypropionate cycle"Appl.Microbiol.Biotechnol.. (In press). (2004)

Masaharu Ishii 等人：“3-羟基丙酸循环的发生、生物化学和可能的生物技术应用”Appl.Microbiol.Biotechnol..（正在出版）。

Masahiro Yamamoto et al.: "Characterization of two differens 2-oxoglutarate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6"Biochem.Biophys.Res.Commun.. 312・4. 1297-1302 (2003)

Masahiro Yamamoto 等人：“两种不同的 2-氧化戊二酸的特性：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem.Biophys.Res.Commun. 312・4 (2003)。

Songkran Chuakrut et al.: "Characterization of a bifunctional Archaeal acyl coenzyme A carboxylase"J.Bacteriol.. 185(3). 938-947 (2003)

Songkran Chuakrut 等人：“双功能古菌酰基辅酶 A 羧化酶的表征”J.Bacteriol.. 185(3)。

Masahiro Yamamoto et al.: "Characterization of two different 2-oxoglutarate : ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6"Biochem.Biophys.Res.Commun.. 312(4). 1297-1302 (2003)

Masahiro Yamamoto 等人：“两种不同 2-氧化戊二酸的表征：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem.Biophys.Res.Commun. 312(4)。

石井正治: "3-ヒドロキシプロピオン酸サイクルで機能する炭酸固定反応"日本農芸化学会誌. 77(4). 413-416 (2003)

Masaharu Ishii：“在 3-羟基丙酸循环中起作用的碳酸固定反应”日本农业化学学会杂志 77(4) (2003)。