Change of E. coli to an autotroph

大肠杆菌转变为自养生物

• 批准号: 24658071
• 负责人: ISHII Masaharu
• 金额: $ 2.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658071/
• 关键词: 大腸菌; 独立栄養; x独立栄養; ヒドロゲナーゼ; 還元的ペントースリン酸サイクル

In order to change E. coli to an autotroph, heterologous expression of RubisCO (Ribulose bisphosphate carboxylase/ oxygenase), PRK (Phosphoribulokinase), and NAD reducing hydrogenase from Ralstonia eutropha were attempted. NAD reducing hydrogenase was successfully expressed under anaerobic growth condition. Also, expression of RubisCO and PRK has been done successfully. Now, final experiment for the checking of the change is being performed.

为了改变E. coli转化为自养菌，尝试异源表达来自真养罗尔斯通氏菌的RubisCO（二磷酸核酮糖羧化酶/加氧酶）、磷酸核酮糖激酶（Phosphoribulokinase）和NAD还原氢化酶。在厌氧条件下成功表达了NAD还原型氢化酶。此外，RubisCO和rubisCO的表达也已成功完成。现在，正在进行检查变化的最后实验。