Basic research for the conversion of carbon dioxide gas into organic resources by using Reductive TCA cycle

还原TCA循环将二氧化碳气体转化为有机资源的基础研究

• 批准号: 12839002
• 负责人: ISHII Masaharu
• 金额: $ 2.37万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12839002/
• 关键词: Reductive TCA cycle; Hydrogen bacterium; Pvnivate : ferrednxin oxidoreductase; 2-Oxoglutarate : ferredoxin oxidoreductase; Pyruvate : ferredoxin oxidoreductase; 2-Oxoglutarate : ferredoxin oxidoreductase(5); Pyruvate:ferredoxin oxidoreductase; 2-Ox

Until recently, because of the shortage of the knowledge about biochemical and/or genetic characters, we have not been able to evaluate the usage of reductive TCA cycle for the production of useful materials. To solve this question, through characterizing the key enzymes of the cycle (Pyruvate : ferredoxin oxidoreductase, and 2-Oxoglutarate : ferredoxin oxidoreductase), basic biochemical information about the cycle for the production was collected and evaluation was carried out.Actual results were as follows ;(1) POR of Hydrogenobacter thermophilus, whose subunit composition was reported to be α・β・γ・δ,was actually composed of five subunits.(2) Two sets of genes for OGOR were obtained and sequenced. Interestingly, the gene was located very closely with different direction of transcription. The genes were just separated by 〜100bp.(3) The genes for OGOR seemed to be regulated by the aerobic/anaerobic condition of the cells.(4) Experiments for RT-PCR for the evaluation of reductive TCA cycle in natural environments were set up. However, distinctive results have not been obtained.

直到最近，由于缺乏对生物化学和/或遗传特性的了解，我们还没有能够评估还原性TCA循环在生产有用材料中的应用。为了解决这一问题，通过对该循环的关键酶（丙酮酸：铁氧还蛋白氧化还原酶，2-葡萄糖酸氧：铁氧还蛋白氧化还原酶）的表征，收集了该循环的基本生化信息，并对其进行了评价。实际结果如下：(1)嗜热氢杆菌的POR，其亚基组成报道为α·β·γ·δ，实际上由5个亚基组成。(2)获得了两组OGOR基因并进行了测序。有趣的是，该基因的位置非常接近，但转录方向不同。基因间距仅为~ 100bp。(3) OGOR基因似乎受细胞的好氧/厌氧条件的调控。(4)建立了评价自然环境下还原性TCA循环的RT-PCR实验。然而，并没有明显的结果。

项目成果

Na-Rae Yun el al.: "The genes for anabolic 2-oxoglutarate : ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6"Biochem. Biophys. Res. Commun.. 282(2). 589-594 (2001)

Na-Rae Yun 等人：“合成代谢 2-氧戊二酸的基因：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem。

Na-Rae Yun el a: "A Novel five-subunit-type 2-oxoglutarate : ferredoxin oxidoreduclase from Hydrogenobacter thermophilus TK-6"Biochem. Biophys. Res, Commun. Accepted.

Na-Rae Yun el a：“一种新型五亚基型 2-氧化戊二酸：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem。

Na-Rae Yun: "A Novel Five-Subunit-Type 2-Oxoglutalate : ferredoxin Oxidoreductases from Hydrogenobacter thermophilus TK-6"Biochem. Biophys. Res. Commun. (Accepted).

Na-Rae Yun：“一种新型五亚基型 2-氧化谷氨酸：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem。

Na-Rae Yun: "A Novel Five-Subunit-Type 2-Oxoglutalate:ferredoxin Oxidoreductases from Hydrogenobacter thermophilus TK-6"Biochem. Biophys. Res. Commun. (Accepted).

Na-Rae Yun：“一种新型五亚基型 2-氧化谷氨酸：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem。

Na-Rae Yun: "The Genes for Anabolic 2-Oxoglutarate : Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6"Biochem. Biophys. Res. Commun. 282・2. 589-594 (2001)

Na-Rae Yun：“合成代谢 2-氧化戊二酸的基因：来自嗜热氢杆菌 TK-6 的铁氧还蛋白氧化还原酶”Biochem.Res.282·594。