权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on the effects of dioxin/dioxin-like congeners on the ovarian function and morphology in the progeny.

二恶英/二恶英类同系物对子代卵巢功能和形态影响的研究。

基本信息

批准号：
14360194
负责人：
SHIROTA Kinji
金额：
$ 5.18万
依托单位：
Azabu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360194/
关键词：
dioxin PCBs endocrine disruptors environmental hormones ovary 内分泌撹乱化学物質

项目摘要

Next results were obtained by this study.1) Dairy oral exposure of female rats to PCB126 from 2weeks before mating until weaning through spontaneous delivery induced direct adverse effects on the growth and gene expression in the ovary of their progeny.2) Single oral exposure of female rats to PCB126 at gestational day 15 decreased the number of oocytes shed at the first ovulation in the progeny, suggesting that the critical point of PCB126 toxicity on the ovary of the progeny might be after gestational day 15 in the rats.3) Single oral exposure of female rats to PCB 126 at gestational day 15 induced decreasing the number of pre-antral and/or antral follicles and impaired expression of the genes associated with steroid-genesis in the ovary, decreasing blood level of inhibin and estradiol, and increasing blood level of FSH in the progeny, suggesting that PCB 126 disturbed follicular growth and steroid production in the ovary of the progeny.4) From the fostering study, either in utero or lactational exposure to PCB 126 induced growth inhibition of the ovarian follicles and decreasing the number of oocytes shed at the first ovulation, but such adverse effects were much more obvious in the lactationally-exposed progeny.5) Single oral exposure to PCB 126 in 24-day-old rats had not any adverse effects on the growth of the large ovarian follicles and the first ovulation, indicating low sensitivity of the ovary at this age to PCB 126.6) It was confirmed that a laser microdissection technique was very useful for sampling and analysis of gene expression of the particular sites in the ovary for searching the target cells of PCB 126.

本研究还获得了以下结果：1）从交配前2周至断乳期间通过自然分娩的方式经口暴露于PCB 126的雌性大鼠，其子代的生长和卵巢基因表达受到直接的不利影响; 2）在妊娠第15天单次经口暴露于PCB 126的雌性大鼠，其子代在第一次排卵时排出的卵母细胞数量减少，3）妊娠15 d的雌性大鼠单次经口暴露PCB 126可导致卵巢中窦前和/或窦状卵泡数量减少，与类固醇生成相关的基因表达受损，结果表明，PCB 126对子代的卵泡发育和卵巢中类固醇激素的产生有一定的干扰作用。无论是在子宫内或哺乳期暴露于PCB 126诱导的卵泡生长抑制，并减少了卵母细胞脱落的数量，5）24日龄大鼠经口暴露PCB 126对大卵泡的生长和首次排卵没有任何不良影响，表明在这个年龄段卵巢对PCB的敏感性较低（126.6）研究证实，激光显微切割技术对于卵巢特定部位的基因表达采样和分析非常有用，可以用于寻找PCB 126的靶细胞。