Pathological analysis of cryoglobulinemia and glomerulonephropathy in experimental Capillaria hepatica infection

本研究通过口服肝毛细线虫卵建立了小鼠实验性冷球蛋白血症模型，结果表明，所诱导的冷球蛋白血症符合以单克隆IgM类风湿因子（RF）和多克隆伊加为特征的II型冷球蛋白血症。实验研究表明，由寄生虫刺激诱导的产生IgM RF的B-1a细胞的选择性增殖发生在冷球蛋白血症的早期阶段，并且还证明了以下因素或事件对于这种冷球蛋白血症的发作是必需的：1）脾B-1a细胞作为IgM RF产生细胞，2）B-1a细胞的增殖，3）特异性寄生虫抗原作为IgM产生的触发物，4）IL-5作为促冷球蛋白血症因子。

This study established the mouse model of experimental cryoglobulinemia induced by oral administration of Capillaria hepatica eggs, and revealed that the induced cryoglobulinemia was consistent with type II cryoglobulinemia which was characterized by monoclonal IgM rheumatoid factor (RF) and polyclonal IgA. The experimental study showed that selective proliferation of IgM RF-producing B-1a cells induced by stimulation of the parasites occurred in the early phase of the cryoglobulinemia, and also demonstrated that following factors or events are essential for the onset of this cryoglobulinemia: 1) the splenic B-1a cells as IgM RF-producing cell, 2) proliferation of B-1a cells, 3) the specific parasitic antigen as a trigger of IgM production, 4) IL-5 as a cryoglobulinemia promoting factor.