STUDY ON THE EFFECTS OF CO-PLANAR PCBS ON THE FUNCTION AND MORPHOLOGY OF FEMALE GENITAL ORGANS

共面PCBS对女性生殖器官功能和形态影响的研究

• 批准号: 12660293
• 负责人: SHIROTA Kinji
• 金额: $ 2.24万
• 依托单位: AZABU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660293/
• 关键词: ENDOCRINE DISRUPTOR; ENVIRONMENTAL HORMONES; PCB; OVARY; MALFORMATION

In experiment (Exp.).I, female SD rats copulated and conceived after daily oral administration for 2 weeks of either corn oil or 1 and 3 μg/kg/day of 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB 126), a congener of coplanar PCBs, respectively. Daily administration was continued until 20 days alter delivery through the gestational period. Exposure to PCB 126 delayed vaginal opening (VO) and reduced the number of oocytes shed at first ovulation at a dose of 3 μg/kg/day. In Exp. II, pregnant female SD rats were single-administered orally either corn oil or 10 and 100 μg/kg of PCB 126 on gestational day 15 (GD 15). A single injection of 10 or 100 μg/kg of PCB 126 to the dams delayed VO and reduced the number of oocytes shed at the first ovulation in their offspring significantly. Most offspring exposed to 100 μg/kg in Exp. II showed VO unsynchronized with the first ovulation in these offspring and reduced uterine weight at the ovulation. In both Exps. I and II, exposed female offspring showed hypospadias. In Exp. III, pregnant female SD rats were single-administered orally either corn oil or 1 and 10 μg/kg of PCB 126 on GD 8 or GD15. Hypospadias was induced in the female offspring exposed to 10 μg/kg. In additional experiment indicate d that lactational exposure to PCB 126 did not induce hypospadias in female offspring. Female offspring exposed to 10 μg/kg of PCB 126 on GD15 showed continuous estrus and reduced ovarian weight at 20 weeks and 30 weeks of age.

在实验（实验）中。1、雌性SD大鼠分别口服玉米油和共面多氯联苯同属物3,3',4,4'，5-五氯联苯（PCB 126） 1和3 μg/kg/d 2周后交配和怀孕。每日给药持续到妊娠期分娩后的第20天。在3 μg/kg/天的剂量下，暴露于PCB 126延迟阴道开口（VO）并减少首次排卵时的卵母细胞脱落数量。实验二：在妊娠第15天（妊娠第15天），给孕雌性SD大鼠单次口服玉米油或10和100 μg/kg的PCB 126。单次注射10或100 μg/kg的PCB 126可显著延缓卵母细胞排卵，减少子代首次排卵时的卵母细胞脱落数。实验ⅱ中剂量为100 μg/kg的子代，其排卵时间与首次排卵时间不同步，排卵时子宫重量减少。在两个exp。ⅰ和ⅱ，暴露的雌性后代表现为尿道下裂。在实验III中，怀孕雌性SD大鼠在GD 8或GD15上单次口服玉米油或1和10 μg/kg PCB 126。暴露于10 μg/kg的雌性子代可诱发尿道下裂。另一项实验表明，哺乳期暴露于PCB 126不会引起雌性后代尿道下裂。在GD15上暴露于10 μg/kg PCB 126的雌性后代在20周龄和30周龄时出现持续发情和卵巢重量减轻的现象。

项目成果

Kenji Yamamoto, Mariko Shirota, Kaoru Inoue, Ari Doyama, Motoko Mukai, Atsuko Haishima, Chie Katoh, Sachie Soda, Atsushi Kawabata, Kohji Shirakura, Yohsuke Sakurada, Fumiaki Akahori, Kinji Shirota: "in utero exposure to 3,3', 4, 4', 5-pentachlorobiphenyl(

Kenji Yamamoto、Mariko Shirota、Kaoru Inoue、Ari Doyama、Motoko Mukai、Atsuko Haishima、Chie Katoh、Sachie Soda、Atsushi Kawabata、Kohji Shirakura、Yohsuke Sakurada、Fumiaki Akhori、Kinji Shirota：“在子宫内暴露于 3,3, 4

MARIKO SHIROTA: "Adverse effects of in utero and lactational exposure to 3,3',4,4',5-pentachrolobiphenyle (PCB126) on the first ovulation in rats"Organohalogen Compounds. 49. 356-358 (2000)

MARIKO SHIROTA：“子宫内和哺乳期接触 3,3,4,4,5-五氯联苯 (PCB126) 对大鼠第一次排卵的不利影响”有机卤素化合物。

Mariko Shirota, Ikue Kitazawa, Kaoru Inoue, Ari Doyama, Motoko Mukai, Atsuko Haishima, Kenji Yamamoto, Chie Katoh, Sachie Soda, Atsushi Kawabata, Fumiaki Akahori, Kinji Shirota: "Adverse effects of in utero and lactational exposure to 3, 3', 4, 4', 5-5-pe

Mariko Shirota、Ikue Kitazawa、Kaoru Inoue、Ari Doyama、Motoko Mukai、Atsuko Haishima、Kenji Yamamoto、Chie Katoh、Sachie Soda、Atsushi Kawabata、Fumiaki Akahori、Kinji Shirota：“子宫内和哺乳期接触 3、3 的不良影响

KENJI YAMAMOTO: "In utero-exposure to 3,3',4,4',5-pentachlorobiphenyl (PCB126) induces hypospadias in female rats"Organohalogen Compounds. 53. 303-305 (2001)

KENJI YAMAMOTO：“子宫内暴露于 3,3,4,4,5-五氯联苯 (PCB126) 会诱发雌性大鼠尿道下裂”有机卤素化合物。