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Development of a noble method for utilization of organic waste resources, open lactic acid fermentation of kitchen refuse is a good model of it

开发有机废弃物资源化利用的高尚方法，餐厨垃圾开放式乳酸发酵就是一个很好的典范

基本信息

批准号：
14360202
负责人：
SAKAI Kenji
金额：
$ 7.94万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In order to show new direction for investigation of economical transformation of organic waste, we set three subjects in the none-sterilizing lactic acid(LA) fermentation of garbage ; analyses of microbes by FISH, mixed culture reactor system with plural substrates, and high-viscous, suspended fermentation system.(1)Quantitative fluorescent In Situ hybridization(FISH) using group- and species- specific probes has established, and selective LA accumulation in the pH-swing control fermentation was found to be caused by L.plantarun, which also resulted decrease of optical purity of LA. On the other hand, inoculation of thermophilic L-LA producing bacteria at 55℃ was effective to accumulation of L-LA. FISH analysis demonstrated well the relationship between LA purity and populations of mesophilic and thermophilic LA bacteria.(2)To determine conditions for supporting growth of a specific microorganism, a new co-culture system with three microfiltration modules, fermentors, and a mixing vess … More el was developed. The co-culture system allowed the cultivation of the individual microorganism in each fermentor with the successful exchange of medium. Efficient production of LA from mixture of xylose and glucose by co-culture with two microorganisms also succeeded. In a simultaneous saccharification and fermentation of unsterile defatted rice bran, a LA bacterium could grow specifically by keeping the pH acidic, with selective LA production.(3)In LA fermentation by Rhizopus oryzae, the high viscosity-suspension system was examined by reducing the water content, however, oxygen supply required for the fermentation was not enough and the LA yield lowered. Product inhibition is strong in the continuous fermentation of glucose. Although starch did not inhibit the fermentation, there was a limit in concentration due to its viscosity. As a result, addition of equivalent water was desirable in kitchen refuse fermentation. Also, it was considered that the substrate containing starch highly should be supplied in batch to the system. Less

为了给有机垃圾的经济转化研究提供新的方向，本研究从垃圾的无菌乳酸发酵、FISH微生物分析、多底物混合培养反应器系统和高粘度悬浮发酵系统三个方面进行了研究。（1）建立了基于组特异性和种特异性探针的定量荧光原位杂交（FISH）技术，发现植物乳杆菌（L.plantarun）在pH摆动控制发酵过程中引起了LA的选择性积累，并导致LA光学纯度的降低。在55℃下接种嗜热L-LA产生菌有利于L-LA的积累。荧光原位杂交分析结果表明，嗜温菌和嗜热菌的数量与乳酸纯度有很好的相关性。(2)To确定支持特定微生物生长的条件，具有三个微滤模块、发酵罐和混合容器的新共培养系统 ...更多信息开发了。共培养系统允许在每个发酵罐中培养单个微生物，并成功更换培养基。利用两种微生物共培养的方法，从木糖和葡萄糖的混合物中高效生产LA也获得了成功。在未灭菌的脱脂米糠的同时糖化和发酵中，LA细菌可以通过保持pH为酸性而特异性地生长，具有选择性的LA生产。(3)In采用根霉（Rhizopus）进行乳酸发酵时，通过降低含水量来考察高粘度悬浮体系，但发酵所需的氧气供应不足，导致乳酸产量降低。葡萄糖连续发酵过程中产物抑制作用较强。虽然淀粉不抑制发酵，但由于其粘度，存在浓度限制。因此，在厨余垃圾发酵过程中，适宜添加等量的水。此外，认为含淀粉高的底物应分批供应到系统中。少