Biological, Epidemiological and Clinical Studies on Amebic Infection among Institutionalized Populations
收容人群中阿米巴感染的生物学、流行病学和临床研究
基本信息
- 批准号:14370085
- 负责人:
- 金额:$ 7.23万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Epidemiological and clinical studies were attempted for residents in institutions, who shoed high infection rates with Entamoeba histolytica. Biological analyses were also conducted on the ameba isolates from such individuals. The epidemiological analyses demonstrated as high as 30% positive rate by the specific antigen detection method and the conventional stool examination. However, most of the infected seemed to be due to the persistent asymptomatic infections with E.histolytica. Several strains of E.histolytica isolated from such individuals with the persistent infection showed genetic and antigenic properties consistent with those of E.histolytica ; however, they did show virtually no virulence against tissue-cultured cells as examined with a sensitive fluorescent method. Based on the base sequence polymorphism of 4 different genes like chitinase gene, identification of genetic types of subpopulations of E.histolytica was developed, and applied to the isolates from several institu … More tions. The analyses demonstrated that the isolates from a single institution showed entirely the same genetic type, suggesting that only single infected individual is responsible for expansion of the amebic infection.Further studies indicated that the gene type at a few institutions with rather high infection rates was exactly the same as that detected in the fist outbreak in Kanagawa Prefecture, and demonstrated that an infected individuals had moved from the institute in Kanagawa to these new institutions. This method was also applied to the ameba isolates from foreign countries lice Thailand. However, virtually no coincidence was detected between these foreign isolates and domestic strains. Trials of axenic cultivation of Entamoeba dispar, nonpathogenic ameba, was also actively conducted, which finally resulted in a newly designed YIGADHA-S medium, which allowed complete axenic cultivation of this ameba. Biological characterization of ameba isolates was also attempted by examining the process of excystation and sulfur containing amino acid metabolism, which resulted in discovery of several novel inhibitors. The possibility of these inhibitors as lead compounds for drug development will be investigated. The experimental model for abscess formation was produced by using a Syrian golden hamster cheek pouch. Less
流行病学和临床研究,试图在机构,谁shoes高感染率与溶组织内阿米巴。对从这些个体分离的阿米巴进行了生物学分析。流行病学分析表明,特异性抗原检测法和常规粪便检查的阳性率高达30%。然而,大多数感染似乎是由于持续无症状感染溶组织肠球菌。从这些持续感染的个体中分离出的几株溶组织肠杆菌显示出与溶组织肠杆菌一致的遗传和抗原特性;然而,用灵敏的荧光方法检查,它们对组织培养细胞几乎没有毒力。根据几丁质酶等4个不同基因的碱基序列多态性,对溶组织艾美耳球虫不同亚群的遗传型进行了鉴定,并应用于几种常见的溶组织艾美耳球虫分离株的鉴定。 ...更多信息 选择。结果表明,同一个机构的分离株基因型完全相同,表明阿米巴感染的扩大是由单个感染者引起的,进一步的研究表明,少数感染率较高的机构的基因型与神奈川县第一次暴发时检测到的基因型完全相同。并证明感染者已从神奈川的研究所转移到这些新的机构。该方法也适用于从国外和泰国分离的阿米巴。然而,这些国外分离株和国内菌株之间几乎没有发现一致性。对非致病性阿米巴--舞帕内阿米巴进行了无菌培养试验,最终设计了一种新的YIGADHA-S培养基,可完全无菌培养该阿米巴。通过研究脱囊和含硫氨基酸代谢过程,对阿米巴分离株进行了生物学表征,发现了几种新的抑制剂。这些抑制剂作为药物开发的先导化合物的可能性将被调查。采用金黄地鼠颊囊制作脓肿形成的实验模型。少
项目成果
期刊论文数量(56)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area
- DOI:10.1128/jcm.40.11.4081-4090.2002
- 发表时间:2002-11-01
- 期刊:
- 影响因子:9.4
- 作者:Haghighi, A;Kobayashi, S;Nozaki, T
- 通讯作者:Nozaki, T
Inhibition of excystation and metacystic development of Entamoeba invadens by the dinitroanilin herbicide oryzalin
二硝基苯胺除草剂安磺灵对入侵内阿米巴的排胞和后囊发育的抑制作用
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Makioka A;Takeuchi T et al.
- 通讯作者:Takeuchi T et al.
竹内 勤, 小林正規, ほか: "寄生虫の院内(施設内)感染対策"厚生労働省医薬局安全対策課;メジカルフレンド社. 184 (2003)
Tsutomu Takeuchi、Masanori Kobayashi 等:“医院内(设施)寄生虫感染的对策”厚生劳动省制药药房安全科第 184 号(2003 年)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Haghighi A, Takeuchi T et al.: "Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geogranphic area"J Clin Microbiol. 40・11. 4081-4090 (2002)
Haghighi A,Takeuchi T 等:“来自有限地理区域的溶组织内阿米巴分离物的显着遗传多态性”J Clin Microbiol 40・11(2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Makioka A, Takeuchi T et al.: "Effect of proteasoiae inhibitors on the growth, encystation and excystation of Entamoeba histolytica and Entamoeba invadens"Parasitol Res. 88・4. 454-459 (2002)
Makioka A、Takeuchi T 等人:“蛋白酶抑制剂对溶组织内阿米巴和侵入内阿米巴的生长、包囊和排泄的影响”Parasitol Res 88·4 (2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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TAKEUCHI Tsutomu其他文献
補骨脂成分の研究(第2報)
骨补充成分的研究(第二次报告)
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
AOMORI Tohru;TSUCHIYA Ayumi;SAKAMOTO Mami;SUZUKI Sayo;JIBIKI Aya;OTSUKA Naoko;ISHIOKA Eriko;KANEKO Yuko;TAKEUCHI Tsutomu;NAKAMURA Tomonori;崔 艶梅,谷口 抄子,黒田 照夫,波多野 力 - 通讯作者:
崔 艶梅,谷口 抄子,黒田 照夫,波多野 力
TAKEUCHI Tsutomu的其他文献
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{{ truncateString('TAKEUCHI Tsutomu', 18)}}的其他基金
Molecular signatures in pre-RA patients by multi-omics analysis
通过多组学分析获得 RA 前期患者的分子特征
- 批准号:
20H03720 - 财政年份:2020
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Identification and characterization of pathogenesis related molecules in early rheumatoid arthritis by DNA microarray
DNA微阵列对早期类风湿性关节炎发病机制相关分子的鉴定和表征
- 批准号:
23390259 - 财政年份:2011
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis on signal transduction pathway through CD103 molecule
CD103分子信号转导通路分析
- 批准号:
20591193 - 财政年份:2008
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Construction of a galaxy SED model consistent with chemical evolution
构建符合化学演化的星系SED模型
- 批准号:
20740105 - 财政年份:2008
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Performance and System Analyses on Indoor Broadband Multimedia Wireless Communication System
室内宽带多媒体无线通信系统性能及系统分析
- 批准号:
19560399 - 财政年份:2007
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanism and its regulation of heterophilic adhesion between α E 3 7 (CD103) and E-cadherin in autoimmune epithelial injury
自身免疫性上皮损伤中α E 3 7 (CD103)与E-cadherin异嗜粘附的分子机制及其调控
- 批准号:
18591122 - 财政年份:2006
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The analyses on indoor wireless environments and the transmission performance of giga-bit wireless LAN
室内无线环境及千兆无线局域网传输性能分析
- 批准号:
15560342 - 财政年份:2003
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanism and possible therapeutic target of BAFF upregulation in SLE
SLE中BAFF上调的分子机制和可能的治疗靶点
- 批准号:
14570426 - 财政年份:2002
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research that factor and morbid state, of Congenital Chagas disease in South America
南美洲先天性恰加斯病的影响因素和发病状况研究
- 批准号:
13576011 - 财政年份:2001
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
International Collaborative Studies on the Control of Soil-Transmitted Helminthiases and Schostosomiasis
土源性蠕虫病和血吸虫病防治国际合作研究
- 批准号:
12800017 - 财政年份:2000
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Special Purposes
相似海外基金
The relevance between growth ability of Entamoeba dispar in tissue and its pathogenicity
迪斯帕内阿米巴在组织中的生长能力与其致病性的相关性
- 批准号:
10670237 - 财政年份:1998
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cell Biological, Epidemiological and Clinical Studies on Entamoeba dispar infection
迪斯帕内阿米巴感染的细胞生物学、流行病学和临床研究
- 批准号:
09470072 - 财政年份:1997
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Establishment of axnic culture of non-invasive Entamoeba dispar and study on its biological characterization
非侵入性迪斯帕内阿米巴轴培养物的建立及其生物学特性研究
- 批准号:
08670287 - 财政年份:1996
- 资助金额:
$ 7.23万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Inaktivierung reaktiver Sauerstoffspezies bei Entamoeba histolytica und Entamoeba dispar. Charakterisierung der Cystein-Proteasen von E. histolytica und E. dispar
溶组织内阿米巴和迪斯帕内阿米巴中活性氧的灭活。
- 批准号:
5305062 - 财政年份:1996
- 资助金额:
$ 7.23万 - 项目类别:
Research Grants