Biological, Epidemiological and Clinical Studies on Amebic Infection among Institutionalized Populations

收容人群中阿米巴感染的生物学、流行病学和临床研究

• 批准号: 14370085
• 负责人: TAKEUCHI Tsutomu
• 金额: $ 7.23万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370085/
• 关键词: Entamoeba histolytica; Entamoeba dispar; amebiasis; infection among institutional population; axenic cultivation; genetic polymorphism; 赤痢アメーバ症; 無症候性感染; 赤痢アメーバ; 病原性; Entasmoeba dispar; Emtamoeba dispar

Epidemiological and clinical studies were attempted for residents in institutions, who shoed high infection rates with Entamoeba histolytica. Biological analyses were also conducted on the ameba isolates from such individuals. The epidemiological analyses demonstrated as high as 30% positive rate by the specific antigen detection method and the conventional stool examination. However, most of the infected seemed to be due to the persistent asymptomatic infections with E.histolytica. Several strains of E.histolytica isolated from such individuals with the persistent infection showed genetic and antigenic properties consistent with those of E.histolytica ; however, they did show virtually no virulence against tissue-cultured cells as examined with a sensitive fluorescent method. Based on the base sequence polymorphism of 4 different genes like chitinase gene, identification of genetic types of subpopulations of E.histolytica was developed, and applied to the isolates from several institu … More tions. The analyses demonstrated that the isolates from a single institution showed entirely the same genetic type, suggesting that only single infected individual is responsible for expansion of the amebic infection.Further studies indicated that the gene type at a few institutions with rather high infection rates was exactly the same as that detected in the fist outbreak in Kanagawa Prefecture, and demonstrated that an infected individuals had moved from the institute in Kanagawa to these new institutions. This method was also applied to the ameba isolates from foreign countries lice Thailand. However, virtually no coincidence was detected between these foreign isolates and domestic strains. Trials of axenic cultivation of Entamoeba dispar, nonpathogenic ameba, was also actively conducted, which finally resulted in a newly designed YIGADHA-S medium, which allowed complete axenic cultivation of this ameba. Biological characterization of ameba isolates was also attempted by examining the process of excystation and sulfur containing amino acid metabolism, which resulted in discovery of several novel inhibitors. The possibility of these inhibitors as lead compounds for drug development will be investigated. The experimental model for abscess formation was produced by using a Syrian golden hamster cheek pouch. Less

流行病学和临床研究，试图在机构，谁shoes高感染率与溶组织内阿米巴。对从这些个体分离的阿米巴进行了生物学分析。流行病学分析表明，特异性抗原检测法和常规粪便检查的阳性率高达30%。然而，大多数感染似乎是由于持续无症状感染溶组织肠球菌。从这些持续感染的个体中分离出的几株溶组织肠杆菌显示出与溶组织肠杆菌一致的遗传和抗原特性;然而，用灵敏的荧光方法检查，它们对组织培养细胞几乎没有毒力。根据几丁质酶等4个不同基因的碱基序列多态性，对溶组织艾美耳球虫不同亚群的遗传型进行了鉴定，并应用于几种常见的溶组织艾美耳球虫分离株的鉴定。 ...更多信息 选择。结果表明，同一个机构的分离株基因型完全相同，表明阿米巴感染的扩大是由单个感染者引起的，进一步的研究表明，少数感染率较高的机构的基因型与神奈川县第一次暴发时检测到的基因型完全相同。并证明感染者已从神奈川的研究所转移到这些新的机构。该方法也适用于从国外和泰国分离的阿米巴。然而，这些国外分离株和国内菌株之间几乎没有发现一致性。对非致病性阿米巴--舞帕内阿米巴进行了无菌培养试验，最终设计了一种新的YIGADHA-S培养基，可完全无菌培养该阿米巴。通过研究脱囊和含硫氨基酸代谢过程，对阿米巴分离株进行了生物学表征，发现了几种新的抑制剂。这些抑制剂作为药物开发的先导化合物的可能性将被调查。采用金黄地鼠颊囊制作脓肿形成的实验模型。少

项目成果

Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area

• DOI: 10.1128/jcm.40.11.4081-4090.2002
• 发表时间: 2002-11-01
• 期刊: JOURNAL OF CLINICAL MICROBIOLOGY
• 影响因子: 9.4
• 作者: Haghighi, A;Kobayashi, S;Nozaki, T
• 通讯作者: Nozaki, T

Inhibition of excystation and metacystic development of Entamoeba invadens by the dinitroanilin herbicide oryzalin

二硝基苯胺除草剂安磺灵对入侵内阿米巴的排胞和后囊发育的抑制作用

• 发表时间: 2002
• 期刊: J Parasitol 88
• 作者: Makioka A;Takeuchi T et al.
• 通讯作者: Takeuchi T et al.

竹内 勤, 小林正規, ほか: "寄生虫の院内(施設内)感染対策"厚生労働省医薬局安全対策課;メジカルフレンド社. 184 (2003)

Tsutomu Takeuchi、Masanori Kobayashi 等：“医院内（设施）寄生虫感染的对策”厚生劳动省制药药房安全科第 184 号（2003 年）

Haghighi A, Takeuchi T et al.: "Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geogranphic area"J Clin Microbiol. 40・11. 4081-4090 (2002)

Haghighi A，Takeuchi T 等：“来自有限地理区域的溶组织内阿米巴分离物的显着遗传多态性”J Clin Microbiol 40・11（2002）。

Makioka A, Takeuchi T et al.: "Effect of proteasoiae inhibitors on the growth, encystation and excystation of Entamoeba histolytica and Entamoeba invadens"Parasitol Res. 88・4. 454-459 (2002)

Makioka A、Takeuchi T 等人：“蛋白酶抑制剂对溶组织内阿米巴和侵入内阿米巴的生长、包囊和排泄的影响”Parasitol Res 88·4 (2002)。