Research that factor and morbid state, of Congenital Chagas disease in South America

南美洲先天性恰加斯病的影响因素和发病状况研究

• 批准号: 13576011
• 负责人: TAKEUCHI Tsutomu
• 金额: $ 8.51万
• 依托单位: KEIO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576011/
• 关键词: Congenital Chaga's disease; Bolivia; Northeast Brazil; Benznidazole; Splenomegaly; Triatoma; Trypanosoma cruzi; Chronic carrier; Bolivia; 媒介昆虫サシガメ; 先天性感染シャーガス病; P.C.R法; xenodiagnosis(媒介体診断); Benzonidazol; 慢性感染; Triatoma; アイソザイム解析; Xenodia

This study started with epidemiological research of congenital Chags' disease in Bolivia, but now evolve to all around in South America including Independencia area in Ceara state in Northeast Brazil. In Bolivia, we could follow up congenital Chagas' babies who had medicated Benznidazole for three years. After medication by Benznidazole, the pathogen disappeared from the blood of more than 95% of neonate, and swelling of the liver and splenomegaly could not be observed, either. But some were detected pathogen from their blood on the third year, we consider the reason uncompleted medication because the contact with the vector (Triatoma) could deny during their follow-up. It is clear that all of the congenital infected mothers are carriers (32/32), but the necessity of treatment has not been observed, so we need to spread knowledge to people including medical workers.From July 24 to 27 in 2003, Miura spoke about the above-mentioned at the 12th Convention Panamericans' NIKKEI-/Health Section (Aug-2003) in Santa Cruz, Bolivia, and apply for support to JICA Partnership Program. There's no case of congenital Chagas' disease in Northeast Brazil but chronic carriers are still exist and also the vector is still living there, so it is possible that this disease would spread as revival infection. The pathogens (Trypanosoma cruzi) which were isolated from the two countries' were different characteristic, we are evaluating about hereditary difference.

这项研究始于玻利维亚的先天性查格斯病的流行病学研究，但现在发展到南美洲各地，包括巴西东北部塞亚州的独立地区。在玻利维亚，我们可以追踪服用苯硝唑三年的先天性查加斯婴儿。经苯硝唑治疗后，95%以上新生儿血中病原体消失，也未见肝脾肿大。但有些人在第三年的时候从他们的血液中检测到了病原体，我们认为没有完成用药的原因是因为在他们的随访中与媒介(Triatoma)的接触可能会被拒绝。很明显，所有先天感染的母亲都是携带者(32/32)，但没有观察到治疗的必要性，因此我们需要向包括医务人员在内的人传播知识。2003年7月24日至27日，三浦在玻利维亚圣克鲁斯举行的第12届泛美人日经/健康大会(2003年8月至27日)上谈到了上述问题，并申请支持日本国际协力事业团伙伴计划。巴西东北部没有先天性恰加斯病病例，但慢性携带者仍然存在，而且媒介仍然生活在那里，因此这种疾病可能会以复发性感染的形式传播。从这两个国家分离到的病原体(克氏锥虫)具有不同的特征，我们正在对遗传差异进行评估。

项目成果

Hamano Sinjiro: "Detection of kinetoplast DNA of Trypanosoma cruzi from dried feces of triatomine"Parasitology International. 50. 135-138 (2001)

Hamano Sinjiro：“从锥虫干粪便中检测克氏锥虫动质体 DNA”国际寄生虫学。

Higo Hiro: "The differences of genetic variation among major lineages in Trypanosoma cruzi"Abstracts of the 72th Annual Meeting of the Japanese Society of Parasitology. Suppl. 112 (2003)

Higo Hiro：“克氏锥虫主要谱系间遗传变异的差异”日本寄生虫学会第72届年会摘要。

Higo Hiro: "Phylogenetic distribution of Trypanosoma cruzi in South America"Abstracts of the 71th Annual Meeting of the Japanese Society of Parasitology. 157・II-D・29 (2002)

Higo Hiro：“南美克氏锥虫的系统发育分布”第71届日本寄生虫学会年会摘要157・II-D・29（2002年）。

Triatomine bugs inhabiting housesin the state of Santa cruz-Bolivia and Northeast portions of Brazil and the infectionrate of Trypanosoma cruzi

玻利维亚圣克鲁斯州和巴西东北部地区房屋中的锥虫及克氏锥虫感染率

• 发表时间: 2005
• 期刊: Jap.Soc.Med.Ent & Zool (In press)
• 作者: Horio Masahiro;Miura Sachio;Takeuchi Tutomu etc.
• 通讯作者: Takeuchi Tutomu etc.

Genetic variation among lineages of Trypanosoma cruzi and its geographic aspects.

克氏锥虫谱系间的遗传变异及其地理方面。

• 发表时间: 2004
• 期刊: Parasitology International 53
• 作者: Higo Hiroo;Miura Sachio;Takeuchi Tsutomu etc.
• 通讯作者: Takeuchi Tsutomu etc.