Reg Gene and Reg receptor Expressed in Human Gastrointestinal Cancers

人类胃肠道癌中表达的 Reg 基因和 Reg 受体

• 批准号: 14370373
• 负责人: UNNO Michiaki
• 金额: $ 8.96万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370373/
• 关键词: REG GENE; REG PROTEIN; REG RECEPTOR; GASTROINTESTINAL CANCER; REG蛋白質; 臨床病理学的検討; Reg; Reg蛋白受容体; 胆管細胞癌; 定量PC; 免疫染色

Reg (Regenerating gene) gene encodes a secretory protein that induces pancreatic beta-cell proliferation as an autocrine/paracrine growth factor via Reg receptor (RegR). Reg protein has been suggested to be involved in regenerative cell proliferation in many tissues such as gastrointestinal, neural and cardiovascular cells.Here, we found a novel human type III REG gene, REGIII. This gene is devided into six exons spanning about 3.0 kbp, and encodes a 175 amino acid protein with 85% homology wiht HIP/PAP. REG III was expressed predominantly in pancreas and testis but not in small intestine, whereas HIP/PAP was expressed strongly in pancreas and small intestine.Now, we established the immunohistochemical analyses for human REG I alfa, REG I beta, REG III, HIP/PAP, REG IV using specific monoclonal antibody. Now, we invetigate the expression of the member of the REG gene family on various gstrointestinal cancers including gastric cancer, colon cancer, pancreatic cancer, liver cancer, and bile duct cancer obtained from surgery. We constructed the database of surgical specimen, including tumor type, the serum level of tumor marker, histological stage, venous invasion, lymph node metastasis, type of recurrence, and prognosis. Though it is analyzing, it is possible that the expression of the member of REG gene family might be associated with the prognosis.

Reg（再生基因）基因编码一种分泌性蛋白，通过Reg受体（RegR）作为自分泌/旁分泌生长因子诱导胰腺β细胞增殖。Reg蛋白被认为参与胃肠道、神经和心血管等多种组织细胞的再生增殖。该基因由6个外显子组成，长度约3.0kbp，编码175个氨基酸，与HIP/PAP同源性为85%。REG Ⅲ主要在胰腺和睾丸中表达，而在小肠中不表达，HIP/PAP在胰腺和小肠中表达较强，本实验采用特异性单克隆抗体建立了人REG Ⅰ α、REG Ⅰ β、REG Ⅲ、HIP/PAP、REG Ⅳ的免疫组化分析。现在，我们研究REG基因家族成员在各种胃肠道癌中的表达，包括胃癌、结肠癌、胰腺癌、肝癌和手术获得的胆管癌。建立手术标本数据库，包括肿瘤类型、血清肿瘤标志物水平、组织学分期、静脉侵犯、淋巴结转移、复发类型和预后。REG基因家族成员的表达可能与胃癌的预后有关。

项目成果

Abe T, Suzuki T, Unno M, et al.: "Thyroid hormone transporters : recent advances"Trends Endocrinol Metab. 13(5). 215-220 (2002)

Abe T、Suzuki T、Unno M 等人：“甲状腺激素转运蛋白：最新进展”趋势内分泌代谢。

Sato K, Sugawara J, et al.: "Expression of Organic Anion Transporting Polypeptide E (OATP-E) in Human Placenta"Placenta. 24(2-3). 144-148 (2003)

Sato K、Sukawara J 等人：“人胎盘中有机阴离子转运多肽 E (OATP-E) 的表达”胎盘。

Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III

• DOI: 10.1016/j.gene.2004.06.010
• 发表时间: 2004-09-29
• 期刊: GENE
• 影响因子: 3.5
• 作者: Nata, K;Liu, Y;Okamoto, H
• 通讯作者: Okamoto, H

マウス胎児肝におけるインスリン遺伝子IとIIの発現

胰岛素基因I和II在小鼠胎肝中的表达

• 发表时间: 2004
• 期刊: 糖尿病 47
• 作者: Sasamori;K.;高沢 伸;高沢 伸;山本靖彦;川口晶子
• 通讯作者: 川口晶子

Cyclin D1 activation through ATF-2 in Reg-induced pancreatic beta-cell regeneration.

• 发表时间: 2006
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: S. Takasawa;T. Ikeda;T. Akiyama;K. Nata;K. Nakagawa;N. Shervani;N. Noguchi;Shoko Murakami-Kawaguchi;A. Yamauchi;I. Takahashi;T. Tomioka-Kumagai;H. Okamoto