Functional recovery of the cerebral tissue following transplantation of the genetically-engineering bone marrow stem cells.

简介：我们已经报道了骨髓细胞移植在脑缺血和脱髓鞘动物模型中的治疗效果。本研究将基因工程骨髓干细胞移植到大脑中动脉闭塞模型大鼠和脑肿瘤模型大鼠体内，研究其潜在的治疗作用。我们已经制备了三种基因工程骨髓干细胞，1）用hTERT基因使干细胞永生化，2）用BDNF基因促进神经营养作用，3）促进IL=2基因的抗肿瘤作用。病变大小进行了评估，使用磁共振成像和光谱，和组织学methods.Results：基因工程骨髓干细胞移植减少病变体积。病灶大小的减少可以在体内与MRI和MRS进行评估，并与随后的组织学检查的brain.Conclusion：这项工作表明，高纯度的基因工程骨髓干细胞在脑缺血模型的神经保护。同时，这些细胞显示出巨大的抗肿瘤作用。因此，骨髓的高度纯化、扩增和基因工程细胞组分已证明在脑缺血和脑肿瘤中的治疗益处。

Introduction : We have reported that transplantation of bone marrow cells has demonstrated therapeutic efficacy in animal models of cerebral ischemia and demyelination. In the present study, we transplanted genetically-engineering bone marrow stem cells into both middle cerebral artery occlusion model rats and brain tumor model rats, to study their potential therapeutic benefit.Methdos : We have made three genetically-engineering bone marrow stem cells, 1)to immortalize the stem cells with hTERT gene, 2)to promote the neurotrophic effects with BDNF gene, 3)to promote the anti-tumor effect with IL=2 gene. Lesion size was assessed using MR imaging and spectroscopy, and histological methods.Results : Transplantation of genetically-engineering bone marrow stem cells reduced lesion volume. The reduction of lesion size could be assessed in vivo with MRI and MRS and was correlated with subsequent histological examination of the brain.Conclusion : This work demonstrates that highly purified genetically-engineering bone marrow stem cells are neuroprotective in cerebral ischemia models. Also, these cells showed the tremendous anti-tumor effects. Thus, a highly purified, expanded, and genetically-engineered cellular component of bone marrow has demonstrated the therapeutic benefits in both cerebral ischemia and brain tumor.