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Functional recovery of the cerebral tissue following transplantation of the genetically-engineering bone marrow stem cells.

基因工程骨髓干细胞移植后脑组织功能恢复。

基本信息

批准号：
14370442
负责人：
HONMOU Osamu
金额：
$ 8.9万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370442/
关键词：
adult gene human infarction neural stem cell stroke transplantation tumor

项目摘要

Introduction : We have reported that transplantation of bone marrow cells has demonstrated therapeutic efficacy in animal models of cerebral ischemia and demyelination. In the present study, we transplanted genetically-engineering bone marrow stem cells into both middle cerebral artery occlusion model rats and brain tumor model rats, to study their potential therapeutic benefit.Methdos : We have made three genetically-engineering bone marrow stem cells, 1)to immortalize the stem cells with hTERT gene, 2)to promote the neurotrophic effects with BDNF gene, 3)to promote the anti-tumor effect with IL=2 gene. Lesion size was assessed using MR imaging and spectroscopy, and histological methods.Results : Transplantation of genetically-engineering bone marrow stem cells reduced lesion volume. The reduction of lesion size could be assessed in vivo with MRI and MRS and was correlated with subsequent histological examination of the brain.Conclusion : This work demonstrates that highly purified genetically-engineering bone marrow stem cells are neuroprotective in cerebral ischemia models. Also, these cells showed the tremendous anti-tumor effects. Thus, a highly purified, expanded, and genetically-engineered cellular component of bone marrow has demonstrated the therapeutic benefits in both cerebral ischemia and brain tumor.

导读：我们已经报道了骨髓细胞移植在脑缺血和脱髓鞘动物模型中显示出治疗效果。在本研究中，我们将基因工程骨髓干细胞移植到大脑中动脉闭塞模型大鼠和脑肿瘤模型大鼠中，研究其潜在的治疗效果。方法：制备3种基因工程骨髓干细胞，1)携带hTERT基因的干细胞永生化，2)携带BDNF基因的干细胞促进神经营养作用，3)携带IL=2基因的干细胞促进抗肿瘤作用。采用磁共振成像、光谱学和组织学方法评估病变大小。结果：基因工程骨髓干细胞移植可减少病变体积。病变大小的减少可以通过MRI和MRS在体内评估，并与随后的脑组织组织学检查相关。结论：高纯度基因工程骨髓干细胞在脑缺血模型中具有神经保护作用。此外，这些细胞还显示出巨大的抗肿瘤作用。因此，高度纯化、扩增和基因工程的骨髓细胞成分已证明对脑缺血和脑肿瘤都有治疗作用。