权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional recovery of the cerebral infarction following transplantation of the genetically-engineering human neural stem cells

基因工程人神经干细胞移植后脑梗死的功能恢复

基本信息

批准号：
12470295
负责人：
HONMOU Osamu
金额：
$ 10.37万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470295/
关键词：
adult gene human infarction neural stem cell stroke transplantation

项目摘要

Introduction: Although it has generally been assumed that the adult brain is incapable of significant self-repair because of a lack of neurogenesis in the adult mammalian central nervous system (CNS), several studies have reported that the adult mammalian brain harbors neural stem cells that retain the potential for both neural production and differentiation in experimental animal models. These findings offer the prospect of the presence of neural precursors in the adult human brain. The objective of this study was to identify neural stem cells in the adult human CNS and study whether they repair the ischemic brain.Methdos: Clonal neural stem cells derived from adult human brain were established in vitro. They were genetically marked with LacZ gene and was transplanted into the ischemic brain of the experimental animal. It was investigated if neural tissue reconstruction occurred following transplantation.Results: Single-cell clonal analysis demonstrated self-renewing and multipotential properties of neural stem cells derived from the adult human brain in vitro. Histological examination of the ischemic lesion following transplantation revealed that the transplanted human cells reconstructed the neural tissue. The transplanted stem cell in the contra-lateral hemisphere also migrated into the ischemic lesion of the another hemisphere, and repaired the damaged brain following ischemic stress. The electrophysiological and behavior studies also proved the functional recovery of the ischemic model animals following the human neural stem cell transplantation.Conclusion: Adult human brain harbors neural stem cells that retain the potential for both neural production and differentiation. Transplantation of clonal neural stem cells derived from adult human brain repaired the ischemic neural tissue in the mammalian adult CNS.

简介：虽然人们普遍认为，由于成年哺乳动物中枢神经系统（CNS）缺乏神经发生，成年大脑无法进行显着的自我修复，但一些研究报告称，成年哺乳动物大脑中含有神经干细胞，在实验动物模型中保留了神经产生和分化的潜力。这些发现为成人大脑中存在神经前体提供了前景。本研究的目的是鉴定成人中枢神经系统中的神经干细胞，并研究它们是否能修复缺血的大脑。方法：体外建立源自成人大脑的克隆神经干细胞。它们被标记了 LacZ 基因，并被移植到实验动物的缺血大脑中。研究了移植后是否发生神经组织重建。结果：单细胞克隆分析证明来自体外成人大脑的神经干细胞具有自我更新和多潜能特性。移植后缺血性病变的组织学检查表明，移植的人类细胞重建了神经组织。对侧半球的移植干细胞也迁移到另一个半球的缺血病灶中，修复缺血应激后受损的大脑。电生理学和行为学研究也证明了人神经干细胞移植后缺血模型动物的功能恢复。结论：成人大脑中含有神经干细胞，保留了神经产生和分化的潜力。来自成人大脑的克隆神经干细胞的移植修复了哺乳动物成人中枢神经系统的缺血神经组织。