Functional recovery of the cerebral infarction following transplantation of the genetically-engineering human neural stem cells

简介：尽管人们普遍认为成年哺乳动物中枢神经系统（CNS）缺乏神经发生，因此成年大脑无法进行显著的自我修复，但一些研究报告称，成年哺乳动物大脑中含有神经干细胞，这些细胞在实验动物模型中保留了神经产生和分化的潜力。这些发现为成人大脑中神经前体的存在提供了前景。本研究的目的是鉴定成年人中枢神经系统中的神经干细胞，并研究其对缺血脑的修复作用。用LacZ基因对它们进行遗传标记，并将其移植到实验动物的缺血脑中。它被调查，如果神经组织重建发生以下transplantation.Results：单细胞克隆分析表明，自我更新和多潜能特性的神经干细胞来源于成年人的大脑在体外。移植后缺血性病变的组织学检查显示，移植的人细胞重建了神经组织。移植到对侧半球的干细胞也迁移到另一侧半球的缺血损伤区，修复缺血应激后受损的脑组织。结论：成年人脑内存在神经干细胞，具有神经生成和分化的潜能。成年人脑神经干细胞移植修复成年哺乳动物中枢神经系统缺血神经组织。

Introduction: Although it has generally been assumed that the adult brain is incapable of significant self-repair because of a lack of neurogenesis in the adult mammalian central nervous system (CNS), several studies have reported that the adult mammalian brain harbors neural stem cells that retain the potential for both neural production and differentiation in experimental animal models. These findings offer the prospect of the presence of neural precursors in the adult human brain. The objective of this study was to identify neural stem cells in the adult human CNS and study whether they repair the ischemic brain.Methdos: Clonal neural stem cells derived from adult human brain were established in vitro. They were genetically marked with LacZ gene and was transplanted into the ischemic brain of the experimental animal. It was investigated if neural tissue reconstruction occurred following transplantation.Results: Single-cell clonal analysis demonstrated self-renewing and multipotential properties of neural stem cells derived from the adult human brain in vitro. Histological examination of the ischemic lesion following transplantation revealed that the transplanted human cells reconstructed the neural tissue. The transplanted stem cell in the contra-lateral hemisphere also migrated into the ischemic lesion of the another hemisphere, and repaired the damaged brain following ischemic stress. The electrophysiological and behavior studies also proved the functional recovery of the ischemic model animals following the human neural stem cell transplantation.Conclusion: Adult human brain harbors neural stem cells that retain the potential for both neural production and differentiation. Transplantation of clonal neural stem cells derived from adult human brain repaired the ischemic neural tissue in the mammalian adult CNS.