A RESERACH ON DEVELOPMENT AND APPLICATION OF THE CLINICALLY APPRICABLE NEW ARTIFICIALRED BLOOD CELLAND ARTIFICIAL OXYGEN CARRIER

血红蛋白（Hb）修饰的人造红细胞由于其一氧化氮（NO）猝灭，存在血液升高或血小板活化、凝血等临床问题不良反应。这是由于血红素分子吸收内皮细胞或神经末梢释放的NO所致。在本研究中，我们开发了一种新的临床应用的Hb修饰分子，其SH部分被s-亚硝基化，因此可以释放NO。此外，我们还开发了一种新的全氟碳（PFC），通过使用一种新的挤奶机，它是在一个相同的大小，稳定和更好的生物利用度融化。我们考察了其作为人工氧载体和人工器官保存剂的生物利用度、临床应用价值。我们计划了NO-PEG-Hb在脑缺血中的临床应用，并确认了其在大鼠2VO模型中的实用性。其机制似乎是抑制iNOS合成的大量NO和NO- peg - hb .2释放的NO。为了将新型PFC作为儿童人工体外循环的血浆扩张器，我们在狗身上进行了模拟实验，并从各种心功能、心律失常和生化参数中认识到它的临床应用价值。我们认识到，通过测量心功能、心律失常和生化参数，新型PFC对心脏缺血再灌注损伤的预防性应用的实用性。为了评价各种人工红细胞对脑缺血保存的作用，我们建立了一种新的大鼠认知评价方法。我们认识到，通过测量心功能、心律失常和生化参数，脂质体包裹的血红蛋白（NRC）对心脏缺血再灌注损伤的预防性应用的实用性。我们认识到NRC对脑缺血的保护作用。用一种大鼠认知功能评价方法。

Hemoglobin (Hb)-modified artificial red blood cell has clinically problematic adverse reactions such as blood elevation or activation of platelets and coagulation due to its nitric oxide (NO) quenching. This is derived from absorption of NO released from endothelial cells or nerve endings by heme of Hb molecule. In the present study we developed a new clinically applicable Hb modified molecule in which its SH moiety is s-nitrosylated, thus, can release NO. Also we developed a new perfluorocarbon (PFC) by using a new milking machine, which is melting in a equal sized, stable and with better bioavailability. We checked its bioavailability, clinical availability as an artificial oxygen carrier and artificial organ preserver.1.We planned the clinical application of NO-PEG-Hb to brain ischemia, and recognized its utility in rat 2VO model. Its mechanism seemed to quench large amount of NO synthesized by iNOS and also NO release from NO-PEG-Hb.2.To utilized the new PFC as a plasma expander for artificial cardiopulmonary bypass in children, we conduced a simulated experiment using dog and recognized its clinical utility from various cardiac functions, arrhythmias and biochemical parameters.3.We recognized that the utility of prophylactic application of the new PFC for cardiac ischemia reperfusion injury by measuring cardiac functions, arrhythmias and biochemical parameters.4.To evaluate effects of various artificial red blood cells on brain ischemia preservation we established a new evaluation method for cognition using the rat.5.We recognized that the utility of prophylactic application of a liposome-encapsulated hemoglobin, Neo Red Cell (NRC), for cardiac ischemia reperfusion injury by measuring cardiac functions, arrhythmias and biochemical parameters.6.We recognized the preserving effect of NRC on brain ischemia. by an evaluation method for cognitive function in the rat.