OPTICAL MAPPING SYSTEM WITH DUAL HIGH SPEED VIDEO CAMERAS FOR OBSERVATION OF THREE DIMENSIONAL CARDIAC EXCITATION PROPAGATION DURING ARRHYTHMIA

具有双高速摄像机的光学绘图系统，用于观察心律失常期间的三维心脏兴奋传播

• 批准号: 14380392
• 负责人: SAKUMA Ichiro
• 金额: $ 11.07万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380392/
• 关键词: Arrhythmia; Reentry; Voltage sensitive dye; Optical Mapping; Cardiac Excitation Mapping; Electrical Stimulation; Virtual electrode polarization; Three Dimensional Shape Measurement; リエントリ; スパイラルリエントリ; 除細動; 膜電位光マッピング; 心筋細胞活動電位; 活動電位三次元表示; 心臓三

Optical mapping has been widely used to investigate the mechanism of induction and termination of cardiac arrhythmia such as ventricular fibrillation and ventricular tachycardia, that are measure causes of sudden cardiac death. In this study, we have the new optical mapping system of cardiac excitation having the following functions :(1)The system can monitor cardiac excitation in wider area of the cardiac surface using two high speed digital video cameras.It can track reentrant activities showing meandering with high temporal and spatial resolution.(2)The system takes three dimensional shape of the heart into consideration to evaluate accurately conduction parameters such as conduction velocity and curvature of wave-front.(3)The system can extract the direction and velocity of wave front or wave-back of the cardiac excitation propagation by means of image processing. This image analysis method is basis for development of adaptive electrical stimulation system that can deliver electric stimuli on arbitrary position and arbitrary time phase depending on the actual cardiac excitation.(4)The system is equipped with electrical stimulation device with transparent electrode module that enables observation of cardiac excitation near the stimulation electrode. The system can monitor virtual electrode polarization phenomena near the stimulation point electrode immediate after the application of the stimuli with higher temporal and spatial resolution than previously reported systems can(5)The system can identify the specific repolarization phase of cardiac tissue at a certain point on cardiac surface in real time, and can deliver the electric stimuli depending on this repolarization phase identification.These engineering achievements enable development of new experimental methodologies that can be applied to cardiac arrhythmia research.

光学标测已被广泛用于研究室颤和室性心动过速等心律失常的诱发和终止机制，这些心律失常是心脏性猝死的可测量原因。在本研究中，我们建立了新的心脏兴奋光学标测系统，该系统具有以下功能：(1)该系统可以使用两个高速数字摄像机在更大范围内监测心脏兴奋，能够以高时间和空间分辨率跟踪呈现曲折的折返性活动。(2)该系统考虑心脏的三维形状，准确地估计传导速度和波前曲率等传导参数。(3)该系统可以通过图像处理提取心脏兴奋传播的波前或波后的方向和速度。这种图像分析方法为自适应电刺激系统的开发奠定了基础，该系统可以根据心脏的实际兴奋情况，在任意位置、任意时间段提供电刺激。(4)该系统配备了带有透明电极模块的电刺激装置，可以在刺激电极附近观察心脏兴奋。该系统可以在施加刺激后立即监测刺激点电极附近的虚拟电极极化现象，具有比以往报道的更高的时间和空间分辨率。(5)该系统能够实时识别心脏组织在心脏表面某一点的特定复极相，并根据该复极相识别出电刺激。这些工程成果为开发可应用于心律失常研究的新的实验方法提供了可能。

项目成果

N.Motoki, 他: "Na+ channel blockade causes a prolongation of electrical diastole during spiral-type reentry in the ventricle"Environmental Medicine. 46. 103-105 (2002)

N. Motoki 等人：“Na+ 通道阻断导致心室螺旋型折返期间电舒张期延长”环境医学 46. 103-105 (2002)。