Cardiac action potential and Ca2+ ion simultaneous dual measurement and temporal and spatial high resolution optical mapping system

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We developed optical mapping system which records two signals simultaneously using single camera by taking each frame synchronized with filter switching. We designed the optical lens system to concentrate fluorescent light at filter position and form image on the CMOS sensor, additionally a custom filter holder for long-pass filter and band-pass filter which is rotated by a motor. Using this system, we observed Vm and Ca2+ dynamics in isolated rabbit heart. Firstly in the first step of basic study, there ware many difficult about the optical design of measuring system. Then we developed another solution for exquisite Vm and Ca dual mapping system that was constructed by 2 camera, dichroic mirror and Mutual Information based registration software both.