Expression of voltage-gated potassium channels in the inner ear and herediatary deafness : molecular biological analysis
电压门控钾通道在内耳和遗传性耳聋中的表达:分子生物学分析
基本信息
- 批准号:14370546
- 负责人:
- 金额:$ 9.02万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.Inwardly rectifying potassium channel Kir4.1 was expressed in the stria vascularis, cochlear supporting cell and satellite cells surround spiral ganglion cells. Kir4.1 was also found in the outer sulcus cells. These results suggest that inwardly rectifying Kir4.1 potassium channel in the outer sulcus cells may play a important role in the potassium ion recycling mechanism via gap junctions.2.In the Brn-4-deficient, Na, K-ATPase-like immunoreactivity in the type II fibrocytes of the cochlear lateral wall was remarkably decreased. Connexin 26 was sparsely distributed among the fibrocytes in the mutant cochlear lateral wall. In contrast, Na, K-ATPase and voltage-gated potassium channel KCNQ1 were abundantly expressed in the marginal cells of the stria vascularis in this mutant. These findings suggest that a dysfunction of fibrocytes in the cochlear lateral wall and an interruption of the K+ ion recycling pathway via gap junctions may result in the depression of endocochlear potential and profound hearing loss in human DFN3 non-syndromic deafness.3.Intense GLAST-like immunoreactivity was found in the type I fibocytes, type II fibrocytes and the suprastrial fibrocytes in the spiral ligament. Glutamine synthetase was abundantly expressed in the type I fibrocytes, the type II fibrocytes, the type III fibrocytes and the suprastrial fibrocytes in the spiral ligament, the basal cells of the stria vascularis. These results suggest that abundant GLAST and glutamine synthetase in the connective tissue cells in the cochlea may function to maintain the perilymphatic glutamate concentration at a low (nontoxic) level.
1.内整流钾通道Kir4.1在血管纹、耳蜗支持细胞和螺旋神经节细胞周围的卫星细胞中表达。Kir4.1也存在于外沟细胞中。这些结果表明,外沟细胞内整流的Kir4.1钾通道可能在通过间隙连接的钾离子循环机制中起重要作用。在brn -4缺失的情况下,耳蜗侧壁II型纤维细胞的Na、k - atp样免疫反应性明显降低。连接蛋白26在突变耳蜗侧壁的纤维细胞中稀疏分布。相比之下,Na、k - atp酶和电压门控钾通道KCNQ1在该突变体血管纹边缘细胞中大量表达。这些发现表明,耳蜗外侧壁纤维细胞功能障碍和通过间隙连接的K+离子循环通路中断可能导致人DFN3非综合征性耳聋的耳蜗内电位抑制和深度听力损失。在螺旋韧带的I型纤维细胞、II型纤维细胞和胃上纤维细胞中发现了强烈的glast样免疫反应。谷氨酰胺合成酶在螺旋韧带、血管纹基底细胞的I型纤维细胞、II型纤维细胞、III型纤维细胞和胃上纤维细胞中大量表达。这些结果表明,耳蜗结缔组织细胞中丰富的谷氨酰胺合成酶和谷氨酰胺合成酶可能起着维持淋巴周围谷氨酸浓度在低(无毒)水平的作用。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Jin Z-H, Kikuchi T, Tanaka K, Kobayashi T: "Expression of glutamate transporter GLAST in the developing mouse cochlea"Tohoku J Exp Med. 200. 137-144 (2003)
Jin Z-H、Kikuchi T、Tanaka K、Kobayashi T:“谷氨酸转运蛋白 GLAST 在发育中的小鼠耳蜗中的表达”Tohoku J Exp Med。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
菊池俊彦: "内耳におけるギャップ結合システムとカリウムイオンリサイクル機構"Otology Japan. (in press).
Toshihiko Kikuchi:“内耳间隙连接系统和钾离子回收机制”Otology Japan(出版中)。
- DOI:
- 发表时间:
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- 影响因子:0
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菊地俊彦: "ラセン靭帯線維細胞とその機能的意義"耳鼻咽喉科展望. (in press). (2004)
Toshihiko Kikuchi:“螺旋韧带纤维细胞及其功能意义”Otorhinolaryngology Perspectives(印刷中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Jin Z-H, Kikuchi T, Tanaka K, Kobayashi T.: "Expression of glutamate transporter GLAST in the developing mouse cochlea"Tohoku J Exp Med. 200. 137-144 (2003)
Jin Z-H、Kikuchi T、Tanaka K、Kobayashi T.:“发育中的小鼠耳蜗中谷氨酸转运蛋白 GLAST 的表达”Tohoku J Exp Med。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
須納瀬弘, 高田雄介, 菊地俊彦, 千葉敏彦, 小林俊光: "繰り返す突発難聴の1症例-聴神経腫瘍との鑑別が必要だった小脳橋角部顔面神経鞘腫-"JOHNS. 20. 529-534 (2004)
Hiroshi Sunose、Yusuke Takada、Toshihiko Kikuchi、Toshihiko Chiba、Toshimitsu Kobayashi:“反复突发性听力损失的病例 - 需要与听神经瘤鉴别的小脑桥脑角面神经神经鞘瘤”JOHNS。
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KIKUCHI Toshihiko其他文献
KIKUCHI Toshihiko的其他文献
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{{ truncateString('KIKUCHI Toshihiko', 18)}}的其他基金
Study on transition support and education support for preschool children with severe motor and intellectual disabilities (SMID) who need high medical care
需要高额医疗护理的严重运动和智力障碍(SMID)学龄前儿童的过渡支持和教育支持研究
- 批准号:
24730762 - 财政年份:2012
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
A study on sense reception evaluation for the children with severe motor and intellectual disabilities(SMID) and minute action expression
重度运动和智力障碍儿童感觉接受评价与微小动作表达的研究
- 批准号:
22730718 - 财政年份:2010
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Medieval North East Asia and Archaeology - Cultural Interchanges and Trends of Peoples in North East Asia around the Nurgan Yongning Temple
中世纪东北亚与考古——努尔干永宁寺周边东北亚民族的文化交流与走向
- 批准号:
19900102 - 财政年份:2007
- 资助金额:
$ 9.02万 - 项目类别:
Analysis of the potassium ion recycling mechanism in the cochlea : a challenge for the better understanding of the inner ear dysfunction
分析耳蜗钾离子循环机制:更好地了解内耳功能障碍的挑战
- 批准号:
16591696 - 财政年份:2004
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of the pathogenesis of the hereditary non syndromic deafness caused by mutations in connection genes
关联基因突变所致遗传性非综合征性耳聋发病机制分析
- 批准号:
12470358 - 财政年份:2000
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional significance of the gap junctions in the ion transport mechanism in the cochlea -molecular biological analysis
间隙连接在耳蜗离子传输机制中的功能意义-分子生物学分析
- 批准号:
10671581 - 财政年份:1998
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (C)