Ex vivo development of a composite human skin equivalent from hair follicles micrografted

1.我们从美国购买了“AlloDerm”，从韩国购买了“SureDerm”。我们决定使用“AlloDerm”用于本项目，因为它在真皮结构和基底膜方面上级此，进行了HE和纤维连接蛋白，胶原IV和层粘连蛋白的免疫组织化学染色。科比大学整形外科没有实验室，最初我们得到了房间，并设置了大型设备，如洁净工作台，热灭菌装置，冷冻离心机，培养箱，冷冻保存容器，工作台和固定的实验设施/仪器. 2003年5月初，我们向科比大学伦理委员会申请了表皮、口腔粘膜、毛囊细胞培养的许可。我们在第二届日本再生医学年会上介绍了两种毛囊培养方法.由于“AlloDerm”的基底膜成分中仅存在Laminin，因此我们将Laminin包被到“AlloDerm”上并尝试毛囊移植，但未成功 ...更多信息 细胞接种，即使采取了它。因此，我们尝试在其他基底膜成分中进行涂层，但后来没有成功。现在我们正在研究一种新的涂层基体预处理方法.另一方面，在科比大学成功地制造了使用“AlloDerm”的复合口腔粘膜，我们在临床应用中取消了伦理委员会，现在重复一个案例（在日本整形外科学会第47届年会上提出）。9.我们于2003年秋季购买了气相色谱仪，仪器设置在三个月内完成，并分析了毛囊和头发的总脂肪酸。棕榈酸大量存在于两者中，必需脂肪酸不存在于毛发中（在第三届日本再生医学年会和第十届瘢痕疙瘩/增生性瘢痕会议上提出）。此外，是目前它在第二届世界伤口愈合协会今年夏天）。10.现在我们正在取样几个月前移植的口腔粘膜，并通过膜脂肪酸分析来审查它们是否在正常粘膜中恢复。少

1. We purchased "AlloDerm" from U.S.A. and "SureDerm" from Korea.2. We decided to utilize "AlloDerm" for this project because that was superior to this about dermal structure and basement membrane, performed HE and immunohistochemical staining of Fibronectin, Collagen IV, and Laminin.3. Kobe University plastic surgery did not have laboratory and at first we got the room and set large apparatus such as clean bench, heat sterilization apparatus, refrigerated centrifuge, incubator, cryopreservation vessel, bench and fixed experiment facilities/instrument.4. We applied to Kobe University ethic committee for allowance of cultivation epidermis, oral mucosa, hair follicle cells at the beginning of 2003.5. We presented two culture methods of hair follicles in the second Annual Meeting Japanese Regenerative Medicine.6. Because only Laminin was present among basement membrane components of "AlloDerm", we coated to "AlloDerm" in Laminin and tried hair follicle transp1antation, but did not succeed … More to cell inoculation even if take did it.7. So, we tried coating in other basement membrane components, but did not succeed afterwards. Now we are under study in attchin and a new coating matrix pretreatment.8. On the other hand, manufacture of the composite oral mucosa which used "AlloDerm" succeeds in Kobe University and we took off an ethic committee in clinical application and now repeats a case (presented it at the 47th Annual Meeting of Japan society plastic and Reconstructive surgery).9. We purchased gas chromatography in autumn, 2003, and instrumental setting completed it in three months and analyzed total fatty acids of hair follicles and hairs. Palmitic acid was a lot present in both, essential fatty acid was not present in hair (presented it in the third Annual Meeting of Japanese Regenerative Medicine, and the tenth keloid/hypertrophic scar meeting for the study. In addition, is to present it in 2nd World Wound Healing Society this summer).10. Now we are sampling the oral mucosa grafted several months ago, and to review whether they recover in normal mucosa through membranous fatty acid analysis. Less