Development of a novel hybrid-acellular nerve graft using hypertonic sodium chloride solution

我们开发了一种利用高渗氯化钠溶液进行脱细胞的新方法。采用HE染色、免疫组织化学染色和电子显微镜图像分析等方法对脱细胞化进行组织学评价。将脱细胞神经移植到大鼠体内，并进行组织学评价。细胞被彻底洗净，细胞外基质保持不变。移植两个月后，雪旺细胞进入移植物，神经丝穿过移植物。这种去细胞神经为周围神经再生提供了一个有用的支架。

We developed novel decellularizing method using hypertonic sodium chloride solution. The histological evaluation for acellularization was performed by HE staining, immunostaining and electron microscope images. The acellular nerve was also transplanted to rats and evaluated histologically. The cells were washed out completely and extracellular matrix was maintained. Two months after the transplantation, Schwann cells thrust into the graft and neurofilament was running through the graft. This acellular nerve presents a useful scaffold for peripheral nerve regeneration.