Development of a novel hybrid-acellular nerve graft using hypertonic sodium chloride solution

我们开发了一种新的脱细胞方法，使用高渗氯化钠溶液。通过HE染色、免疫组化染色和电镜图像对去细胞化进行组织学评价。将脱细胞神经移植到大鼠体内并进行组织学评价。细胞被完全洗出并保持细胞外基质。移植后两个月，雪旺细胞进入移植物，神经丝贯穿移植物。这种脱细胞神经为周围神经再生提供了有用的支架。

We developed novel decellularizing method using hypertonic sodium chloride solution. The histological evaluation for acellularization was performed by HE staining, immunostaining and electron microscope images. The acellular nerve was also transplanted to rats and evaluated histologically. The cells were washed out completely and extracellular matrix was maintained. Two months after the transplantation, Schwann cells thrust into the graft and neurofilament was running through the graft. This acellular nerve presents a useful scaffold for peripheral nerve regeneration.