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Lipid analysis of cell membranes in normal dermis, hypertrophic and keloidal scars

正常真皮、肥厚性疤痕和疤痕疙瘩细胞膜的脂质分析

基本信息

批准号：
17390476
负责人：
TERASHI Hiroto
金额：
$ 10.71万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390476/
关键词：
Scar Keloid Fatty acid Dermis Wound healing Arachidonic acid 無血清培養方法

项目摘要

Hypertrophic scars (HS) are the consequence of abnormal wound healing. The basic mechanism of HS formation is still unknown; however; a number of factors involved in HS formation have been revealed: the mast cells, TGF-beta, and cultured fibroblasts. Compared with normal skin, HS demonstrate four-fold increase in the numbers of mast cells. Although HS produce excess collagen, fibroblasts from HS and normal skin have similar proliferation rates. Fibroblasts derived from HS behave more like those from normal scars than those from keloids by not exhibiting an exaggerated response to TGF-□ We focused on fatty acids involved in HS on the hypothesis that some lipids influence HS formation and maintenance. Several investigators have described the interactions between lipids and scarring and wound healing. We examined fatty acids that are contained within, and participate in every reaction through membrane; then we analyzed the percentage composition of the fatty acids in de-epithelialized normal dermis (ND) and HS. In vivo HS samples were obtained from 6 patients undergoing surgical excision, and ND samples from 5 patients undergoing skin grafting surgery for excess. In vitro, cultured fibroblasts from HS and ND were also analyzed. The percentage composition of fatty acids extracted from all the samples was analyzed. In vivo, arachidonic acid (20:4) was significantly more abundant in HS than in ND, in the phospholipids from both whole tissue and cell membrane. In vitro, there were no significant difference among ND, HS, and 10% fetal calf serum (FCS). The results suggest that HS formation does not necessarily involve simple excess of 20:4-acid; however, there are considerable differences in percentage composition of 20:4-acid between ND and HS. Arachidonic acid probably participates in the formation and maintenance of HS, whereas in vitro cultured fibroblasts are affected largely by FCS.

肥厚性疤痕（HS）是伤口愈合异常的结果。 HS形成的基本机制尚不清楚；然而;已经揭示了许多参与 HS 形成的因素：肥大细胞、TGF-β 和培养的成纤维细胞。与正常皮肤相比，HS 的肥大细胞数量增加了四倍。尽管 HS 产生过量的胶原蛋白，但 HS 和正常皮肤的成纤维细胞具有相似的增殖率。源自 HS 的成纤维细胞的行为比源自疤痕疙瘩的成纤维细胞更类似于来自正常疤痕的成纤维细胞，因为它们没有表现出对 TGF-□ 的过度反应。我们基于某些脂质影响 HS 形成和维持的假设，将重点放在与 HS 相关的脂肪酸上。一些研究人员描述了脂质与疤痕和伤口愈合之间的相互作用。我们检查了其中包含的脂肪酸，并通过膜参与每个反应；然后我们分析了去上皮正常真皮（ND）和HS中脂肪酸的百分比组成。体内HS样本取自6名接受手术切除的患者，ND样本取自5名接受过量植皮手术的患者。体外培养的 HS 和 ND 成纤维细胞也进行了分析。分析了从所有样品中提取的脂肪酸的百分比组成。在体内，整个组织和细胞膜的磷脂中，花生四烯酸 (20:4) 在 HS 中的含量显着高于 ND。在体外，ND、HS和10%胎牛血清（FCS）之间无显着差异。结果表明 HS 的形成并不一定涉及简单过量的 20:4-酸；然而，ND和HS之间20:4-酸的百分比组成存在相当大的差异。花生四烯酸可能参与HS的形成和维持，而体外培养的成纤维细胞主要受FCS影响。