A defense mechanism of a living body in the odontoblastic complex via gap junction

成牙本质细胞复合体中活体通过间隙连接的防御机制

• 批准号: 14370614
• 负责人: IKEDA Hideharu
• 金额: $ 5.5万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370614/
• 关键词: odontoblast; gap junction; dye coupling; electrical coupling; tooth pulp; dentinal tubules; dentinal fluid flow; tooth pain; 象牙芽細胞; Gap junction; イオンチャンネル; 象牙細管; transjunctional conductance; 生体防御; 象牙細管内容液の流れ; 象牙質細胞; gap-junction; 歯髄; 細芽細

In our series of studies, we electrophysiologically obtained the function of odontoblasts and a direct, non-neural transduction mechanism between odontoblasts and between odontoblasts and neighboring cells. We recognized dentinal tubules as that have a close relation with odontoblasts as the pathway for several stimuli, and focused on the defense mechanism of outward flow of dentinal fluid, microcirculation of dental pulp and chronic dental pain.We revealed1.Dye coupling using low molecule dye2.Rack of evidence that there were voltage dependent currents in feline odontoblasts3.Defense mechanism of the outward flow against the LPS entry through dentinal tubules of intact and caries affected dentin4.Evidence of a functional syncytium of odontoblast complex5.Effect of aging on electrical conductance between odontoblasts6.Effect of extracellular pH on electrical conductance between odontoblasts7.Effect of mechanical distortion of odontoblast cell membrane electrical conductance between odontoblast8.Application of whole-cell voltage-clamp method to feline odontoblast9.Gap junction between human odontoblasts and neighboring cells10.Effect of iontophoresis and pulpward hydrostatic pressure on drug delivery through dentinal tubules 111.Effect of pulpal components on dentin permeability12.Changes in human sensation and neural activity with aging13.Neuropathic orofacial pain evoked by cold stimulus of oral mucosa14.Microcirculation in the dental pulp

在我们的一系列研究中，我们从电生理学角度获得了成牙本质细胞的功能，以及成牙本质细胞之间以及成牙本质细胞与邻近细胞之间直接的、非神经的信号转导机制。我们认为牙本质小管是与成牙本质细胞关系密切的细胞，是多种刺激的通道，并着重探讨了牙本质液外流的防御机制，本研究揭示了：1.低分子染料的染料偶联; 2.成牙本质细胞存在电压依赖性电流的证据不足; 3.成牙本质细胞外向电流对脂多糖进入牙本质细胞的防御机制完整和龋齿影响牙本质的小管4.成牙本质细胞复合体功能合胞体的证据5.老化对成牙本质细胞间电导的影响6.细胞外pH值对成牙本质细胞间电导的影响7.成牙本质细胞膜机械变形对成牙本质细胞间电导的影响8.全细胞电压-猫成牙本质细胞的钳夹法9.人成牙本质细胞与邻近细胞之间的缝隙连接10.离子导入和向髓静水压力对药物通过牙本质小管的影响111.牙髓成分对牙本质的影响渗透性12.人的感觉和神经活动随年龄的变化13.冷刺激口腔粘膜引起的神经性口面疼痛14.牙髓微循环

项目成果

Facilitation of iontophoretic drug delivery through intact and caries-affected dentine

• DOI: 10.1046/j.1365-2591.2003.00712.x
• 发表时间: 2003-10-01
• 期刊: INTERNATIONAL ENDODONTIC JOURNAL
• 影响因子: 5
• 作者: Puapichartdumrong, P;Ikeda, H;Suda, H
• 通讯作者: Suda, H

池田 英治: "歯髄の微小循環"ザ クインテッセンス. 22・1. 139-149 (2003)

池田英二：“牙髓的微循环”精髓22・1（2003）。

Outward fluid flow reduces inward diffusion of bacterial lipopolysaccharide across intact and demineralised dentine.

• DOI: 10.1016/j.archoralbio.2004.12.004
• 发表时间: 2005-08
• 期刊: Archives of oral biology
• 影响因子: 3
• 作者: P. Puapichartdumrong;P. Puapichartdumrong;H. Ikeda;H. Suda

「Dental Pulp」に学ぶ, 1.The circulation of the pulp

学习“牙髓”，1.牙髓的循环

• 发表时间: 2003
• 期刊: Quintessence 22(1)
• 作者: Ikeda H;Akiyama G;Matsuzaki S;Sato M;Koshikawa N;Cools AR.;Ikeda H
• 通讯作者: Ikeda H

Effect of mechanical distortion of odontoblast cell membrane on cell-to-cell communication in the odontoblastic layer.

成牙本质细胞膜机械变形对成牙本质细胞层细胞间通讯的影响。

• 发表时间: 2003
• 期刊: J Dent Res 82(Spec Iss), B-47
• 作者: IKEDA H;SUDA H
• 通讯作者: SUDA H