A study on pulp lymphatic system using multiphoton-excitation microscopy and analysis of membrane potential-sensitivity

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(1) Lymphangiogenesis caused by experimentally induced pulpitis : Pulpal inflammation was induced by applying bacterial irritants to rat exposed dentin. Changes in distribution and density of lymphatic vessels were observed using two-photon microscopy following specific antibodies(VEGFR-3, LYVE-1, Prox-1). Images showed lymphangiogenesis-like change.(2) Measurement of functional space of fluid movement in the dentinal tubules : Small fluorescent microspheres(diameter : 0. 1-0. 2μm) could pass the space between dentinal tubules and cellular components in the human dentinal tubules. But big microspheres could not reach dental pulp despite the outward tapering of dentinal tubules.