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Experimental studies on oral tissue regeneration by cell, cytokine and bio-absorbable scaffold material

细胞、细胞因子和生物可吸收支架材料口腔组织再生的实验研究

基本信息

批准号：
14370643
负责人：
ARAKI Yoshima
金额：
$ 8.77万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

The findings obtained are described in the following four categories.(1)Studies of cell culture for tissue re-generation were conducted. Bone marrow stromal cells and mesenchymal stem cells were better cultured with ME medium for cell proliferation, and were efficiently differentiated into osteoblasts with DME medium supplemented with vitamin C, Dex and beta-glycerol phosphate. Effects of metal ions and LPS on cytokine and cell toxicity on the cells were also evaluated. DNA microarray analysis clarified that growth factors having ligands for TGF-beta super-family receptor might be useful for guiding osteogenic differentiation.(2)Studies of scaffold materials for tissue re-generation were performed. Bio-absorbable collagen, gelatin and poly-glycol acid were self-formulated into sponge and hydrogen forms, and their bio-absorption and chemical cross-linking were studied. Collagen sponges were useful as scaffold for tissue-regeneration and cell seeding.(3)Studies of animal experiments were carried out. Bio-absorption of scaffold materials were evaluated. Experimental defects in animal calvaria were recovered by implantation of bio-absorbable scaffold materials (acidic gelatins) having slow-release growth factors.(4)Studies of alkaline-heat-treated titanium were done. Apatite layer formed by immersion in artificial body fluid contained carbonate, resembled natural bone, and significantly accelerated osteogenic differentiation of osteoblasts.

所获得的发现分为以下四类：(1)进行了细胞培养用于组织再生的研究。骨髓基质细胞和间充质干细胞在ME培养基中可以更好地培养细胞增殖，并在添加了维生素C、Dex和β-甘油磷酸盐的DME培养基中有效地分化为成骨细胞。还评估了金属离子和LPS对细胞因子和细胞毒性的影响。 DNA微阵列分析表明，具有TGF-β超家族受体配体的生长因子可能有助于指导成骨分化。(2)组织再生支架材料的研究。将生物可吸收的胶原蛋白、明胶和聚乙二醇酸自行配制为海绵和氢形式，并研究了它们的生物吸收和化学交联。胶原海绵可作为组织再生和细胞接种的支架。(3)进行了动物实验研究。评估了支架材料的生物吸收。通过植入具有缓释生长因子的生物可吸收支架材料(酸性明胶)来修复动物颅骨的实验缺陷。(4)进行了碱热处理钛的研究。浸泡在人造体液中形成的磷灰石层含有碳酸盐，类似于天然骨，显着加速成骨细胞的成骨分化。