Molecular mechanisms of adipocyte differentiation.

脂肪细胞分化的分子机制。

基本信息

批准号：
14370749
负责人：
IMAGAWA Masayoshi
金额：
$ 8.83万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370749/
关键词：
Obesity Adipocyte Diabetes Differentiation Transcription Factor Molecular Biology Biochemistry 生化学

项目摘要

The events at the beginning of adipocyte differentiation are not well known. Using a subtraction method, we previously isolated 102 genes, which are expressed in the early stage of adipocyte differentiation of mouse 3T3-L1 cells. In this study, we characterized these genes.1. Both RGS2 and TCL/TC10βL have crucial roles in the early stage of adipocyte differentiation, probably linked to the PPARγ pathway.2. About forty genes seem to be a novel gene, since there is no significantly similar gene listed in databases. Therefore, we isolated the full-length cDNAs and named fad (factor for adipocyte differentiation).3. Both mouse and human fad158 encode 803 amino acids, and contain four transmembrane regions and eight leucine-rich repeat motifs. By sense and antisense experiments, we found that fad158 has the ability to regulate adipocyte differentiation positively, especially at an early stage.4. The expression of fad24 was rapidly induced after the induction. FAD24 was found in the nucleus, … More especially in nuclear speckles. There is a possibility that FAD24 functions as one of the components for the transcription and/or pre-mRNA splicing and positively regulates adipocyte differentiation.5. A novel gene named fadl04, whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 has revealed the possible presence of a fibronectin type III domain and transmembrane domain, and functions as a positive regulator of adipogenesis.6. Fad123 is identical to SLC39A14, a member of the LZT proteins, one of the subfamilies of ZIP transporters. The zinc uptake assay revealed that SLC39A14 functions as a zinc transporter.Thus, we newly identified the genes, which were induced during adipogenesis. Some genes have crucial roles on adipocyte differentiation. However, it is unclear whether the results obtained here are conserved in vivo. To elucidate these important points, the development of knockout mice is now in progress. Less

脂肪细胞分化开始时的事件尚不清楚。使用减法方法，我们先前分离出102个基因，这些基因在小鼠3T3-L1细胞的脂肪细胞分化的早期表达。在这项研究中，我们表征了这些基因1。 RGS2和TCl/TC10βL在脂肪细胞分化的早期阶段都具有至关重要的作用，可能与PPARγ途径有关。2。大约有40个基因似乎是一个新的基因，因为数据库中没有列出的显着类似基因。因此，我们分离了全长cDNA并命名为FAD（脂肪细胞分化的因子）3。小鼠和人类FAD158都编码803个氨基酸，并包含四个跨膜区域和八个富含亮氨酸的重复基序。通过有义务和反义实验，我们发现FAD158具有积极调节脂肪细胞分化的能力，尤其是在早期阶段。4。诱导后，FAD24的表达迅速诱导。 FAD24在核中发现，尤其是在核斑点中。 FAD24可能是转录和/或MRNA剪接的组件之一，并积极调节脂肪细胞分化。5。一个名为FADL04的新型基因，其表达水平在脂肪生成的早期阶段很快升高。推导的FAD104的氨基酸序列揭示了纤连蛋白III型结构域和转运蛋白结构域的可能存在，并且起作用为脂肪形成的阳性调节剂6。 FAD123与SLC39A14相同，SLC39A14是LZT蛋白的成员，这是拉链转运蛋白的亚家族之一。锌摄取测定法表明SLC39A14充当锌转运蛋白。因此，我们新鉴定出在脂肪形成期间诱导的基因。一些基因在脂肪细胞分化中具有至关重要的作用。但是，目前尚不清楚此处获得的结果是否在体内保守。为了阐明这些要点，现在正在进行敲除小鼠的发展。较少的