Molecular mechanisms of adipocyte differentiation.

脂肪细胞分化是由一个复杂的基因阵列被称为主调控。我们之前分离了102个基因，这些基因在脂肪细胞分化的早期阶段表达。在这项研究中，我们鉴定了已鉴定的基因，包括fads（脂肪细胞分化因子），这是未知的基因。fad158的敲低和过表达实验表明，fad158具有正向调节脂肪细胞分化的能力，尤其是在早期阶段。fad104的表达水平在脂肪形成早期迅速升高。FAD104显示可能存在纤维连接蛋白III型结构域和跨膜结构域。我们已经培育了fad104基因敲除小鼠并进行了鉴定。fad24基因的敲低抑制有丝分裂克隆扩增（MCE）。此外，FAD24与HBO1相互作用，HBO1是一种组蛋白乙酰转移酶和DNA复制起始的正调节因子，并与HBO1协同作用，促进脂肪生成。我们还分离出了新的基因fad49。RNA干扰实验显示该基因在脂肪细胞分化过程中也有重要作用。此外，fad49可能在脂肪细胞分化过程中起到支架蛋白的作用。一个未知的基因被鉴定为印迹基因，peg（父系表达基因）10。通过RNA干扰敲低peg10抑制3T3-L1细胞向脂质脂肪细胞的分化。有趣的是，peg10 rnai处理降低了C/EBPβ和C/EBPδ的表达，并抑制了mce。细胞周期蛋白D2也从未知基因中鉴定出来。另一种d型细胞周期蛋白cyclin D1和cyclin D3在脂肪细胞分化早期也被短暂诱导表达。每种d型细胞周期蛋白似乎都通过调节MCE在脂肪细胞分化中发挥关键作用。因此，我们鉴定了新发现的基因，其表达在脂肪细胞分化的早期迅速增加。一些基因在脂肪形成中起着至关重要的作用。少

Adipocyte differentiation is known to be regulated by a complex array of genes known as master regulators. We previously isolated 102 genes, which are expressed in the early stage of adipocyte differentiation. In this study, we characterized identified genes including fads (factor for adipocyte differentiation), which are unknown genes.1. The knockdown and over-expression experiments for fad158 revealed that fad158 has the ability to regulate adipocyte differentiation positively, especially at an early stage.2. The expression level of fad104 quickly increased in the early stage of adipogenesis. FAD104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. We have developed fad104 knockout mice and characterizing now.3. The knock down of fad24 repressed mitotic clonal expansion (MCE). Moreover, FAD24 interacts with HBO1, a histone acetyltransferase and positive regulator of DNA replication initiation, and acts in concert with HBO1 to promote adipogenes … More is by controlling DNA replication.4. We also isolated the new gene, fad49. The RNA interference assay revealed that this gene also has some critical roles on adipocyte differentiation. Moreover, fad49 may function as a scaffold protein during adipocyte differentiation.5. One unknown gene was identified as an imprinted gene, peg (paternally expressed gene) 10. The knockdown of peg10 by RNA interference inhibited the differentiation of 3T3-L1 cells into lipid-laden adipocytes. Interestingly, peg10 RNAi-treatment reduced the expressions of C/EBPβ and C/EBPδ, and inhibited MCE.6. Cyclin D2 was also identified from unknown genes. The expression of cyclin D1 and cyclin D3, the other D-type cyclins, was also transiently induced early during adipocyte differentiation. Each of the D-type cyclins seems to play a crucial role in adipocyte differentiation by regulating MCE..Thus, we characterized newly identified genes, whose expression increased rapidly at the early stage of adipocyte differentiation. Some genes have crucial roles in adipogenesis. Less