Molecular mechanisms of adipocyte differentiation.

脂肪细胞分化的分子机制。

基本信息

批准号：
17390023
负责人：
IMAGAWA Masayoshi
金额：
$ 10.33万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390023/
关键词：
Obesity Adipocyte Diabetes Differentiation Transcrintion Factor Molecular Biology Biochemistry

项目摘要

Adipocyte differentiation is known to be regulated by a complex array of genes known as master regulators. We previously isolated 102 genes, which are expressed in the early stage of adipocyte differentiation. In this study, we characterized identified genes including fads (factor for adipocyte differentiation), which are unknown genes.1. The knockdown and over-expression experiments for fad158 revealed that fad158 has the ability to regulate adipocyte differentiation positively, especially at an early stage.2. The expression level of fad104 quickly increased in the early stage of adipogenesis. FAD104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. We have developed fad104 knockout mice and characterizing now.3. The knock down of fad24 repressed mitotic clonal expansion (MCE). Moreover, FAD24 interacts with HBO1, a histone acetyltransferase and positive regulator of DNA replication initiation, and acts in concert with HBO1 to promote adipogenes … More is by controlling DNA replication.4. We also isolated the new gene, fad49. The RNA interference assay revealed that this gene also has some critical roles on adipocyte differentiation. Moreover, fad49 may function as a scaffold protein during adipocyte differentiation.5. One unknown gene was identified as an imprinted gene, peg (paternally expressed gene) 10. The knockdown of peg10 by RNA interference inhibited the differentiation of 3T3-L1 cells into lipid-laden adipocytes. Interestingly, peg10 RNAi-treatment reduced the expressions of C/EBPβ and C/EBPδ, and inhibited MCE.6. Cyclin D2 was also identified from unknown genes. The expression of cyclin D1 and cyclin D3, the other D-type cyclins, was also transiently induced early during adipocyte differentiation. Each of the D-type cyclins seems to play a crucial role in adipocyte differentiation by regulating MCE..Thus, we characterized newly identified genes, whose expression increased rapidly at the early stage of adipocyte differentiation. Some genes have crucial roles in adipogenesis. Less

已知脂肪细胞分化受称为主要调节剂的复杂基因的调节。我们先前分离了102个基因，这些基因在脂肪细胞分化的早期阶段表达。在这项研究中，我们表征了包括FAD（脂肪细胞分化的因子），这是未知基因1。 FAD158的敲低和过表达实验表明，FAD158具有积极调节脂肪细胞分化的能力，尤其是在早期阶段2。 FAD104的表达水平在成生成的早期阶段迅速增加。 FAD104揭示了纤连蛋白III型结构域和跨膜结构域的可能存在。我们已经开发了FAD104淘汰小鼠并现在表征3。 FAD24的敲低抑制有丝分裂克隆扩张（MCE）。此外，FAD24与HBO1相互作用，HBO1是一种组蛋白乙酰转移酶和DNA复制计划的阳性调节剂，并与HBO1一起起作用以促进脂肪细胞的分化……更多是通过控制DNA复制。4。我们还隔离了新基因FAD49。 RNA干扰测定法表明该基因在脂肪细胞分化方面也具有一些关键作用。此外，FAD49在脂肪细胞分化过程中可能充当支架蛋白。5。一个未知的基因被鉴定为一个烙印基因PEG（亲子表达的基因）10。RNA干扰对PEG10的敲低抑制了3T3-L1细胞分化为脂肪脂肪细胞的分化。有趣的是，PEG10 RNAI处理降低了C/EBPβ和C/EBPδ的表达，并抑制了MCE.6。还从未知基因中鉴定出细胞周期蛋白D2。在脂肪细胞分化期间，其他D型细胞周期蛋白（其他D型细胞周期蛋白）的表达也瞬时诱导。每种D型细胞周期蛋白似乎通过反思MCE来在脂肪细胞分化中起着至关重要的作用。因此，我们表征了新鉴定的基因，在脂肪细胞分化的早期，其表达迅速增加。一些基因在脂肪细胞分化中具有至关重要的作用。较少的