Development of the method to diagnose the prognosis of autoimmune disease by clarifying the pathologic factors.

为了建立自身免疫性疾病预后的诊断方法，我们对可能决定自身免疫性疾病发病机制和预后的各种免疫调节细胞和分子进行了研究，明确如下：1.在自身免疫性甲状腺疾病中，调节性CD25 CD4细胞和NKT细胞在甲状腺中的比例低于血液。活化的细胞毒性T细胞和甲状腺自身抗体独立参与桥本病甲状腺功能减退的发生，CD30CD8细胞上CD30表达的减少也参与了桥本病甲状腺功能减退的发生。3.IgG3分泌细胞的数量和IL-10水平与抗甲状腺药物治疗Graves病的难治性有关。4.活化的蛋白C抵抗与抗蛋白抗体S抗体是非系统性红斑狼疮患者深静脉血栓形成的重要危险因素。且与SLE患者抗凝血酶原抗体和狼疮抗凝活性共存有关。

To develop the diagnostic method for the prognosis of autoimmune disease, we investigated various immunoregulatory cells and molecules which may determine the pathogenesis and the prognosis of autoimmune disease, and clarified as follows.1.In autoimmune thyroid disease, the proportions of regulatory CD25+CD4+ cells and NKT cells were lower in thyroid than in blood, and there was a possibility that FasL+CD4+CD8+ dendritic cells may cause apoptosis of Fas+CD4+ cells in thyroid.2.Activated cytotoxic T cells and thyroid autoantibodies were independently involved in the development of hypothyroidism in Hashimoto's disease and a decrease of CD30 expression on CD30+CD8+ cells was also involved in this development.3.The number of IgG3-secreting cells and the level of IL-10 were involved in the intractability of Graves' disease under treatment with anti-thyroid drugs.4.Acquired activated protein C resistance was associated with anti-protein S antibody as a strong risk factor for deep vein thrombosis in non-SLE patients, and was associated with the co-existence of anti-prothrombin antibodies and lupus anticoagulant activity in SLE patients.