权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular cloning and functional analysis of Arabidopsis thaliana genes involved in bromovirus multiplication

拟南芥雀斑病毒增殖相关基因的分子克隆及功能分析

基本信息

批准号：
15380035
负责人：
MISE Kazuyuki
金额：
$ 10.24万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

(1)Cassia yellow blotch bromovirus(CYBV) was shown to infect systemically and efficiently a model plant species, Arabidopsis thaliana, as we recently reported for another bromovirus, Spring beauty latent bromovirus(SBLV). CYBV and SBLV showed different infectivities in some accessions of A.thaliana. Full-length cDNA clones of CYBV genomic RNAs were constructed, from which infectious in vitro transcripts can be transcribed. (2)Symptom development was examined in A.thaliana accessions infected with SBLV. Several accessions including S96 were sensitive to SBLV infection, while the other accessions including Col-0 were symptomless or developed mild symptoms. Genetic study suggested that the symptom phenotype of S96 is controlled by a single semidominant locus. We have designated this locus SSB1. Infectivity of SBLV in plants and protoplasts were comparable between Col-0 and S96, indicating that variation of symptom phenotype was not due to the different infectivity of SBLV. By using genetic markers, SSB1 was mapped between CAT2 and nga1107 on chromosome IV. Genetic analyses suggest that symptom development in A.thaliana controlled by SSB1 is dependent on salicylic acid signaling and ethylene signaling. (3)Brome mosaic bromovirus(BMV) shows limited infection in A.thaliana, while SBLV efficiently infects it. In a screening of Arabidopsis mutants with altered defense responses, BMV was found to infect A.thaliana systemically and efficiently by the cpr5 mutations, which have been known to constitutively express systemic acquired resistance(SAR). Double and triple mutants of cpr5 with these pathways disrupted still allowed BMV to infect A.thaliana efficiently, suggesting that cpr5 mutations enhance BMV multiplication by the mechanism independent of that to confer SAR. Even in protoplasts, multiplication ability of BMV was lower than that of SBLV and was enhanced by the cpr5 mutations.

(1)决明子黄斑病毒(Cassia Huang Botch BromoVirus，简称CyBV)能系统有效地侵染模式植物拟南芥，与我们最近报道的另一种模式植物拟南芥(Arabitopsis Thaliana)--春花潜伏雀麦病毒(Spring Beauty Minent BromoVirus，SBLV)一样。在拟南芥的部分种质上表现出不同的侵染性。构建了CYBV基因组RNA的全长cDNA克隆，可在体外转录出具有感染性的转录本。(2)对感染SBLV的拟南芥进行了症状发生观察。包括S96在内的几个材料对SBLV感染敏感，而包括Col-0在内的其他材料没有症状或症状较轻。遗传研究表明，S96的症状表型受单个半显性基因座控制。我们已将该基因座命名为SSB1。SBLV在植物和原生质体中的侵染力与Col-0和S96相当，表明症状表型的差异不是由于SBLV的侵染性不同造成的。利用遗传标记将SSB1定位在第4染色体上的CAT2和nga1107之间。遗传分析表明，SSB1控制的拟南芥症状的发生依赖于水杨酸信号和乙烯信号。(3)雀麦花叶雀麦病毒(BMV)对拟南星天牛的侵染有限，而大豆花叶病毒(SBLV)能有效侵染该病毒。在对具有防御反应改变的拟南芥突变体的筛选中，BMV被发现通过cpr5突变系统而有效地感染拟南芥，cpr5突变已被认为是结构性表达系统获得性抗性(SAR)。Cpr5的双突变体和三突变体在这些途径被破坏后仍能使BMV有效地侵染拟南星天牛，这表明Cpr5突变促进了BMV的增殖，其机制不依赖于赋予SAR的机制。即使在原生质体中，BMV的增殖能力也低于SBLV，cpr5突变可增强BMV的增殖能力。