Analysis of molecular interactions between a novel bromovirus and Arabidopsis thaliana

新型雀麦病毒与拟南芥之间的分子相互作用分析

基本信息

批准号：
13660049
负责人：
MISE Kazuyuki
金额：
$ 2.24万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660049/
关键词：
Plant virus Bromovirus Arabidopsis thaliana Full-length cDNA Mutant Cell-to-cell movement Symptom Chromosome ウイルス全身感染 in vitro転写 Tissue-printing

项目摘要

Brome mosaic virus (BMV) is one of the most-studied plant virues, but does not infect model plants such as Arabidopsis thaliana. We have recently found that a related bromovirus, Spring beauty latnet virus (SBLV) systemically and efficiently infects A. thaliana. To establish a system for the anlysis of molecular interactions between SBLV and A. thaliana, we first constructed biologically active cDNA clones of SBLV genomic RNAs and determined their complete nucleotide sequences. To identify plant factor(s) involved in SBLV infection, (1) We inoculated 2,231 M2 (Col-0 background) plants mutagenized with EMS treament and identified one mutart line in which SBLV infection was delayed The SBLV infection in this line was shownto be affected at the step of the cell-to-cell movement Genetic analysis demonstrated that slower SBLVspread in this line was recessive. (2) We inoculated 67 Arabidopsis accesions with SBLV, and found anaccession, Pla-O in which SBLV spread was delayed at least in incculated leaves. The SBLV infection in Pla-0 was shown to be affected At the step of the cell-to-cell raovement rather than maltiplication within a single (cell.Genetic analysis demonstrated that slower SBLV spread in Pla-0 was recessive or semidominant trait. (3) Symptom development was examined in A. thaliana accenssions infected with SBLV. Of 85 Arabidopsis accessions tested, four accessions including an accession S96 were Scnsitive to SBLV infection, while the otner accessions including Col-0 were symptomless or developed only mild symptoms. Genetic study suggested that the symptom phenotype of S96 is controlled by a single semidominant lolcus. We bave designated this locus SSBI. Infectivity of SBLV in plants and protoplasts were compalable between Col-0and S96, indicating that variation of symptom phenotype was not due to the different infectivity of SBLV By using genetic markers, SSBI was mapped between ATMB3R and JM142 on chromosome IV.

Brome Mosaic病毒（BMV）是最研究的植物病毒之一，但不感染诸如拟南芥之类的模型植物。我们最近发现，相关的溴维特病毒，春季美感Latnet病毒（SBLV）系统地感染了A. thaliana。为了建立SBLV和A. thaliana之间分子相互作用的厌食的系统，我们首先构建了SBLV基因组RNA的生物活性cDNA克隆，并确定了它们的完整核苷酸序列。为了确定参与SBLV感染的植物因子，（1）我们接种了2,231 M2（Col-0背景）植物，该植物用EMS治际诱变，并确定了一条MUTART系列，其中SBLV感染延迟了SBLV感染该系列的SBLV感染在该系列中显示出在细胞对细胞移动分析的阶段中受到遗传分析的影响。（2）我们用SBLV接种了67个拟南芥，并发现了无pla-o，其中SBLV扩散至少在不合格的叶片中延迟。 The SBLV infection in Pla-0 was shown to be affected At the step of the cell-to-cell raovement rather than maltiplication within a single (cell.Genetic analysis demonstrated that slower SBLV spread in Pla-0 was recessive or semidominant trait. (3) Symptom development was examined in A. thaliana accenssions infected with SBLV. Of 85 Arabidopsis accessions tested, four accessions including an accession S96对SBLV感染具有SCN，而包括col-0的OTNE入门是无症状的，或者仅发出的遗传研究表明，S96的症状表型是由单个半主导的LOLCUS控制的。表型不是由于使用遗传标记的SBLV的不同感染性，而是在IV染色体上的ATMB3R和JM142之间映射SSBI。