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Analysis of molecular interactions between a novel bromovirus and Arabidopsis thaliana

新型雀麦病毒与拟南芥之间的分子相互作用分析

基本信息

批准号：
13660049
负责人：
MISE Kazuyuki
金额：
$ 2.24万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660049/
关键词：
Plant virus Bromovirus Arabidopsis thaliana Full-length cDNA Mutant Cell-to-cell movement Symptom Chromosome ウイルス全身感染 in vitro転写 Tissue-printing

项目摘要

Brome mosaic virus (BMV) is one of the most-studied plant virues, but does not infect model plants such as Arabidopsis thaliana. We have recently found that a related bromovirus, Spring beauty latnet virus (SBLV) systemically and efficiently infects A. thaliana. To establish a system for the anlysis of molecular interactions between SBLV and A. thaliana, we first constructed biologically active cDNA clones of SBLV genomic RNAs and determined their complete nucleotide sequences. To identify plant factor(s) involved in SBLV infection, (1) We inoculated 2,231 M2 (Col-0 background) plants mutagenized with EMS treament and identified one mutart line in which SBLV infection was delayed The SBLV infection in this line was shownto be affected at the step of the cell-to-cell movement Genetic analysis demonstrated that slower SBLVspread in this line was recessive. (2) We inoculated 67 Arabidopsis accesions with SBLV, and found anaccession, Pla-O in which SBLV spread was delayed at least in incculated leaves. The SBLV infection in Pla-0 was shown to be affected At the step of the cell-to-cell raovement rather than maltiplication within a single (cell.Genetic analysis demonstrated that slower SBLV spread in Pla-0 was recessive or semidominant trait. (3) Symptom development was examined in A. thaliana accenssions infected with SBLV. Of 85 Arabidopsis accessions tested, four accessions including an accession S96 were Scnsitive to SBLV infection, while the otner accessions including Col-0 were symptomless or developed only mild symptoms. Genetic study suggested that the symptom phenotype of S96 is controlled by a single semidominant lolcus. We bave designated this locus SSBI. Infectivity of SBLV in plants and protoplasts were compalable between Col-0and S96, indicating that variation of symptom phenotype was not due to the different infectivity of SBLV By using genetic markers, SSBI was mapped between ATMB3R and JM142 on chromosome IV.

雀麦花叶病毒 (BMV) 是研究最多的植物病毒之一，但不会感染拟南芥等模型植物。我们最近发现一种相关的雀麦病毒——春美拉特网病毒（SBLV）能够系统、有效地感染拟南芥。为了建立分析 SBLV 和拟南芥之间分子相互作用的系统，我们首先构建了 SBLV 基因组 RNA 的生物活性 cDNA 克隆，并确定了其完整的核苷酸序列。为了鉴定参与 SBLV 感染的植物因子，(1) 我们接种了 2,231 株经 EMS 诱变的 M2（Col-0 背景）植物，并鉴定了一个 SBLV 感染延迟的突变品系。该品系中的 SBLV 感染在细胞间移动的步骤中受到影响。遗传分析表明，该品系中较慢的 SBLV 传播是隐性的。 (2)我们用SBLV接种了67个拟南芥种质，发现了一种种质Pla-O，其中SBLV传播至少在接种的叶片中被延迟。 Pla-0 中的 SBLV 感染被证明是在细胞间扩散阶段受到影响，而不是在单个细胞内发生错倍。遗传分析表明，Pla-0 中 SBLV 传播较慢是隐性或半显性性状。(3) 在感染 SBLV 的拟南芥种质中检查症状的发展。在测试的 85 个拟南芥种质中，有 4 个种质包括 1 个种质。 S96对SBLV感染敏感，而包括Col-0在内的其他品种则无症状或仅出现轻微症状。遗传学研究表明，S96 的症状表型是由单个半显性位点控制的。我们已指定该位点 SSBI。 Col-0 和 S96 之间 SBLV 在植物和原生质体中的感染性具有可比性，表明症状的变化表型并非由于SBLV的感染性不同所致。利用遗传标记，SSBI被定位在IV号染色体上的ATMB3R和JM142之间。