Studies on eukaryotic inorganic carbon transporters based on the gene network involved in photosynthetic acclimation

点击翻译按钮获取中文摘要

Chlamydomonas modulates photosynthetic characteristics to acclimate to CO_2-limiting stress by sensing CO_2 availability and light by inducing a set of genes for carbon-concentrating mechanism. We have analyzed regulation of a gene Cah1 encoding a carbonic anhydrase to elucidate the CO_2-signal transduction pathways. By using Ars-reporter fused with Cah1 promoter, cis-acting elements and DNA-binding proteins involved in CO_2-regulation were elucidated (Kucho et al. Plant Physiol. 2003, Yoshioka et al. Plant Cell, 2004). Genome wide analysis of gene expression of regulatory mutants revealed that CO_2-responsive genes were grouped into several categories (Miura Plant Physiol., 2004). Although functions of a master regulator CCM1 (Fukuzawa et al. PNAS 2001) is not known, several low-CO_2 inducible genes encoding chloroplast envelop proteins and anion transporters were good candidates for inorganic carbon transporters in eukaryotic algae, Chlamydomonas reinhardtii.