Studies on eukaryotic inorganic carbon transporters based on the gene network involved in photosynthetic acclimation

基于光合驯化基因网络的真核无机碳转运蛋白研究

• 批准号: 15380071
• 负责人: FUKUZAWA Hideya
• 金额: $ 4.86万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380071/
• 关键词: Carbon dioxide; Photosynthetic carbon fixation; nitrate transporter; Chloroplast envelope; DNA array; Zinc finger; Gree algae, Chlamydomonas; Signal transduction; クラミドモナス; cDNAアレイ; 無機炭素濃縮機構; 低CO_2ストレス; シグナル伝達; トランスポーター

Chlamydomonas modulates photosynthetic characteristics to acclimate to CO_2-limiting stress by sensing CO_2 availability and light by inducing a set of genes for carbon-concentrating mechanism. We have analyzed regulation of a gene Cah1 encoding a carbonic anhydrase to elucidate the CO_2-signal transduction pathways. By using Ars-reporter fused with Cah1 promoter, cis-acting elements and DNA-binding proteins involved in CO_2-regulation were elucidated (Kucho et al. Plant Physiol. 2003, Yoshioka et al. Plant Cell, 2004). Genome wide analysis of gene expression of regulatory mutants revealed that CO_2-responsive genes were grouped into several categories (Miura Plant Physiol., 2004). Although functions of a master regulator CCM1 (Fukuzawa et al. PNAS 2001) is not known, several low-CO_2 inducible genes encoding chloroplast envelop proteins and anion transporters were good candidates for inorganic carbon transporters in eukaryotic algae, Chlamydomonas reinhardtii.

衣藻通过诱导一组碳浓缩机制基因来感应 CO_2 可用性和光，从而调节光合特性以适应 CO_2 限制胁迫。我们分析了编码碳酸酐酶的基因 Cah1 的调控，以阐明 CO_2 信号转导途径。通过使用与Cah1启动子融合的Ars报告基因，阐明了参与CO_2调节的顺式作用元件和DNA结合蛋白（Kucho等人Plant Physiol.2003，Yoshioka等人Plant Cell，2004）。对调节突变体基因表达的全基因组分析表明，CO_2 响应基因可分为几类（Miura Plant Physiol., 2004）。尽管主调节器 CCM1（Fukuzawa 等人，PNAS 2001）的功能尚不清楚，但编码叶绿体包膜蛋白和阴离子转运蛋白的几个低 CO_2 诱导基因是真核藻类莱茵衣藻中无机碳转运蛋白的良好候选者。

项目成果

二酸化炭素による転写調節機構-緑藻クラミドモナスのCO_2濃縮機構

绿藻衣藻中二氧化碳-CO_2浓度机制的转录调控机制

• 发表时间: 2005
• 期刊: 蛋白質 核酸 酵素(共立出版) 50巻8号(印刷中)
• 作者: 福澤秀哉;山野隆志
• 通讯作者: 山野隆志

Yoshioka S., et al.: "A Novel Myb Transcription Factor LCR1 Regulates the CO_<2-> Responsive Gene Cah1 Encoding A Periplasmic Carbonic Anhydrase in Chlamydomonas reinhardtii"The Plant Cell. 16. 1466-1477 (2004)

Yoshioka S.等人：“新型Myb转录因子LCR1调节莱茵衣藻中编码周质碳酸酐酶的CO_2-响应基因Cah1”植物细胞。

Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii

• DOI: 10.1104/pp.104.041400
• 发表时间: 2004-07-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Miura, K;Yamano, T;Fukuzawa, H
• 通讯作者: Fukuzawa, H

Mechanism of CO_2-responsive transcriptional regulation in photosynthetic organisms : Carbon-concentrating mechanism in a green algae, Chlamydomonas reinhardtii

光合生物中CO_2响应转录调控的机制：绿藻莱茵衣藻的碳浓缩机制

• 发表时间: 2005
• 期刊: TANPAKUSHITSU-KAKUSAN-KOUSO(in Japanese) (in press)
• 作者: Hideya Fukuzawa;Takashi Yamano
• 通讯作者: Takashi Yamano

Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta) to facilitate gene function analysis

• DOI: 10.2216/i0031-8884-43-6-722.1
• 发表时间: 2004-11-01
• 期刊: PHYCOLOGIA
• 影响因子: 1.6
• 作者: Asamizu, E;Nakamura, Y;Tabata, S
• 通讯作者: Tabata, S