Identification and utilization of genes for carbon-concentratingmechanism and hydrogen production in microalga by digital expression analyses.

藻类光合作用是生物材料生产的基础，由碳浓缩机制(CCM)提供支持。为了确定CCM相关基因，使用从不同胁迫条件下培养的细胞制备的不同RNA样本进行RNA-seq分析，包括低CO2或氮饥饿条件。利用RNA-seq数据和基因组序列数据，我们建立了一个新的绿藻表达数据库。通过比较低CO2胁迫条件、氮饥饿条件和厌氧条件下的RNA表达谱，确定了诱导表达的基因。在转基因衣藻细胞中人工过表达了几个CCM相关基因，其光合作用特性较野生型细胞有明显改善。

Algal photosynthesis, which is base for production of bio-materials, is supported by carbon-concentrating mechanism (CCM). To identify CCM-related genes, RNA-seq analyses using different RNA samples prepared from cells cultured in various stress conditions including low-CO2 or nitrogen starved conditions. Using RNA-seq data and genomic sequence data, we have developed a new expression database for green alga, Chlamydomonas reinhardtii. Genes which are induced in low-CO2 stress conditions, nitrogen-starved conditions, and anaerobic conditions were identified by comparing the RNA expression profiles. Several CCM-related genes were artificially overexpressed in transgenic Chlamydomonas cells and the photosynthetic characteristics were show to be significantly improved than wild type cells.