Identification and utilization of genes for carbon-concentratingmechanism and hydrogen production in microalga by digital expression analyses.

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Algal photosynthesis, which is base for production of bio-materials, is supported by carbon-concentrating mechanism (CCM). To identify CCM-related genes, RNA-seq analyses using different RNA samples prepared from cells cultured in various stress conditions including low-CO2 or nitrogen starved conditions. Using RNA-seq data and genomic sequence data, we have developed a new expression database for green alga, Chlamydomonas reinhardtii. Genes which are induced in low-CO2 stress conditions, nitrogen-starved conditions, and anaerobic conditions were identified by comparing the RNA expression profiles. Several CCM-related genes were artificially overexpressed in transgenic Chlamydomonas cells and the photosynthetic characteristics were show to be significantly improved than wild type cells.