Using microbubbles to increase the destruction of liver tumors by high-intensity focused ultrasound

利用微泡通过高强度聚焦超声增加对肝脏肿瘤的破坏

• 批准号: 15390228
• 负责人: MARUYAMA Toshiyuki
• 金额: $ 8.13万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390228/
• 关键词: HIFU; microbubble; tumor necrosis; development of liver tumors; coagulated volume; 焼灼体積; 経門脈的投与; 肝臓癌モデルrat

We studied the possibility of using high-intensity focused ultrasound (HIFU) together with a microbubble agent to treat hepatocellular carcinoma.The transducer for HIFU was 40 mm in diameter ; it produced a continuous 2.18-MHz wave for 30 seconds.The spatial peak temporal average intensity was 600 W/cm2.Development of liver tumors in rats was induced by administration of Dimethylnitrosamin (100 ppm).Rats with liver tumors were anesthetized, underwent laparotomy, and were given the microbubble agent Levovist or saline intravenously.After 60 seconds of Levovist or saline injection, the liver was exposed to HIFU for 30 seconds. Immediately after HIFU exposure, ultrasound images of the HIFU area were evaluated. Then the liver was excised and the volume of coagulated tissue was measured.The mean volume of hyperechoic areas after HIFU were as follows (mm3, Levovist vs. saline : 355.3 ± 180.7 vs. 47.4 ± 35.6, p < 0.001, n=13).The volumes of liver tissue coagulated by HIFU were as follows (mm3, Levovist vs. saline : 275.3 ± 120.0 vs. 60.1 ± 23.6, p < 0.001, n=13).On microscopic examination of areas exposed to HIFU, implosion cysts were seen, and many cancer cells were found to have been disrupted or destroyed completely.Some tumor cells had been distorted by loss of cell membranes or nuclei.In conclusion, the microbubble agent Levovist, by increasing the effect of HIFU, could also have a direct role in anti-cancer therapy.

我们研究了使用高强度聚焦超声（HIFU）与微泡剂一起治疗肝细胞癌的可能性。HIFU 的换能器直径为 40 毫米；它产生连续的2.18-MHz波30秒。空间峰值时间平均强度为600 W/cm2。通过给予二甲基亚硝胺(100 ppm)诱导大鼠肝肿瘤的发展。将患有肝肿瘤的大鼠麻醉，进行剖腹手术，并静脉注射微泡剂Levovis或生理盐水。60秒后 Levovist 或盐水注射后，肝脏暴露于 HIFU 30 秒。 HIFU 暴露后，立即评估 HIFU 区域的超声图像。然后切除肝脏并测量凝固组织的体积。HIFU后高回声区域的平均体积如下（mm3，Levovis vs.生理盐水：355.3±180.7 vs.47.4±35.6，p <0.001，n=13）。HIFU凝固的肝组织体积如下（mm3，Levovis vs.生理盐水：355.3±180.7 vs.47.4±35.6，p <0.001，n=13）。 275.3 ± 120.0 vs. 60.1 ± 23.6, p < 0.001, n=13)。在显微镜检查暴露于 HIFU 的区域时，可以看到内爆囊肿，发现许多癌细胞已被完全破坏或破坏。一些肿瘤细胞因细胞膜或细胞核的丢失而扭曲。综上所述，微泡剂 Levovist 通过增强 HIFU 也可以在抗癌治疗中发挥直接作用。

项目成果

Use of a microbubble agent to increase the effects of high intensity focused ultrasound on liver tissue

• DOI: 10.1007/s00330-005-2663-7
• 发表时间: 2005-07-01
• 期刊: EUROPEAN RADIOLOGY
• 影响因子: 5.9
• 作者: Kaneko, Y;Maruyama, T;Matsumoto, Y
• 通讯作者: Matsumoto, Y

Polyacrylamide gel containing egg white as new model for irradiation experiments using focused ultrasound

• DOI: 10.1016/j.ultrasmedbio.2004.07.016
• 发表时间: 2004-10-01
• 期刊: ULTRASOUND IN MEDICINE AND BIOLOGY
• 影响因子: 2.9
• 作者: Takegami, K;Kaneko, Y;Nagawa, H
• 通讯作者: Nagawa, H

18F-FDG PET for hepatocellular carcinoma presenting with portal vein thrombus.

18F-FDG PET 用于诊断伴有门静脉血栓的肝细胞癌。

• 发表时间: 2005
• 期刊: Journal of Gastroenterology. 40
• 作者: Hanajiri K;Mitsui H;Maruyama T;Kondo Y;Shiina S;Omata M;Nakagawa K.
• 通讯作者: Nakagawa K.

Hepatitis C virus core protein activates ERK and p38 MAPK in cooperation with ethanol in transgenic mice

• DOI: 10.1002/hep.1840380408
• 发表时间: 2003-10
• 期刊: Hepatology
• 影响因子: 13.5
• 作者: T. Tsutsumi;Tetsuro Suzuki;K. Moriya;Y. Shintani;H. Fujie;H. Miyoshi;Y. Matsuura;K. Koike;T. Miyamura

Anti-HBs antibodies produced after liver transplantation : from the donor or the recipient?

肝移植后产生的抗-HBs抗体：来自供者还是受者？

• 发表时间: 2003
• 期刊: Hepatology 38
• 作者: Maruyama T;et al.
• 通讯作者: et al.