Cell cycle regulation for treatment of rheumatoid arthritis

细胞周期调节治疗类风湿性关节炎

• 批准号: 15390311
• 负责人: KOHSAKA Hitoshi
• 金额: $ 7.81万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390311/
• 关键词: rheumatoid arthritis; cell cycle; cyclin-dependent kinase inhibitor; 増殖抑制; ノックアウトマウス; 遺伝子治療

Objective. Forced expression of cyclin-dependent kinase inhibitor (CDKI) gene, p16INK4a or p21Cip1 in the synovial tissues was effective in treating animal models of rheumatoid arthritis (RA). Although the primary function of CDKIs is halting cell cycle progression, their anti-proliferative effect accompanied suppression of inflammation in the treated joints. Subsequently, we and others found that p21 Cip1 does not only inhibit activitity of CDKs but downmodulates c-Jun N-terminal kinase (JNK) and other intracellular signaling pathways. Despite lack of solid evidence for interaction of p16INK4a with other molecules, p16INK4a suppressed specific inflammatory molecules produced by rheumatoid synovial fibroblasts (RSF). Since, some of these molecules were shared by the effects of p16INK4a and p21 Cip1, direct effects of CDK4/6 activity on their production were studied. Methods. Genes for CDKIs, p16INK4a and p18INK4c, a constitutively active, hypophosphorylated form of retinoblastoma gene … More product (Rb), cyclin D1 and CDK4 were transferred into RSF with adenoviruses to modulate CDK4/6 activity. A synthetic CDK4/6 inhibitor was used to inhibit CDK4/6. Cell cycle was studied with 3H-thymidine incorporation and flow cytometry. Expression levels of matrix metalloproteinase (MMP)-3, monocyte chemoattaractant protein (MCP)-1 or type I interleukin 1 receptor (IL-1R1) was determined with Northern blot analyses, real-time polymerase chain reaction, and/or ELISAs. CDKIs were immunoprecipitated to reveal their association with JNK. Results. Gene transfer of p16INK4a as well as p18INK4c inhibited cell cycle progression and suppressed MMP-3 and MCP-1 production. Unlike p21Cip1, neither molecules inhibit IL-1 R1 expression or bind to JNK. Inhibition of CDK4/6 by a synthetic CDK4/6 inhibitor also downregulated these inflammatory mediators. They were upregulated when CDK4 activity was augmented. This regulation functioned at the mRNA level for MMP-3, but not for MCP-1. Transfer of active Rb, which mimicked inhibition of CDK-dependent Rb phosphorylation, suppressed production of both without changing their mRNA levels. Conclusions. Inhibition of CDK4/6 in RSF downregulated MCP-1 and MMP-3 production, which was upregulated by augmented CDK4 activity. MMP-3 production was primarily regulated by the kinase activity at the mRNA level in an Rb-independent manner, while MCP-1 production was controlled posttranscriptionally by Rb. Less

客观的。在滑膜组织中强制表达细胞周期蛋白依赖性激酶抑制剂（CDKI）基因p16INK4a或p21Cip1可有效治疗类风湿性关节炎（RA）动物模型。尽管 CDKI 的主要功能是阻止细胞周期进展，但它们的抗增殖作用伴随着抑制治疗关节中的炎症。随后，我们和其他人发现 p21 Cip1 不仅抑制 CDK 的活性，而且下调 c-Jun N 末端激酶 (JNK) 和其他细胞内信号通路。尽管缺乏 p16INK4a 与其他分子相互作用的确凿证据，但 p16INK4a 抑制了类风湿滑膜成纤维细胞 (RSF) 产生的特定炎症分子。由于其中一些分子受到 p16INK4a 和 p21 Cip1 的影响，因此研究了 CDK4/6 活性对其产生的直接影响。方法。将 CDKI 基因、p16INK4a 和 p18INK4c（视网膜母细胞瘤基因产物 (Rb) 的组成型活性、低磷酸化形式）、细胞周期蛋白 D1 和 CDK4 与腺病毒一起转移到 RSF 中，以调节 CDK4/6 活性。使用合成的 CDK4/6 抑制剂来抑制 CDK4/6。使用 3 H-胸苷掺入和流式细胞术研究细胞周期。通过 Northern blot 分析、实时聚合酶链式反应和/或 ELISA 测定基质金属蛋白酶 (MMP)-3、单核细胞趋化蛋白 (MCP)-1 或 I 型白细胞介素 1 受体 (IL-1R1) 的表达水平。对 CDKI 进行免疫沉淀以揭示其与 JNK 的关联。结果。 p16INK4a 和 p18INK4c 的基因转移抑制细胞周期进程并抑制 MMP-3 和 MCP-1 的产生。与 p21Cip1 不同，这两种分子都不会抑制 IL-1 R1 表达或与 JNK 结合。合成 CDK4/6 抑制剂对 CDK4/6 的抑制也下调了这些炎症介质。当 CDK4 活性增强时，它们的表达上调。这种调节在 MMP-3 的 mRNA 水平上起作用，但对 MCP-1 不起作用。活性 Rb 的转移模拟了 CDK 依赖性 Rb 磷酸化的抑制，抑制了两者的产生，但不改变其 mRNA 水平。结论。 RSF 中 CDK4/6 的抑制下调了 MCP-1 和 MMP-3 的产生，而 CDK4 活性的增强则上调了 MCP-1 和 MMP-3 的产生。 MMP-3 的产生主要由 mRNA 水平上的激酶活性以不依赖 Rb 的方式调节，而 MCP-1 的产生则由 Rb 转录后控制。较少的

项目成果

Iwai H, Kohsaka H et al.: "Involvement of inducible costimulator-B7 homologous protein costimulatory pathway in murine lupus nephritis."J Immunol. 171(9). 2848-2854 (2003)

Iwai H、Kohsaka H 等人：“小鼠狼疮性肾炎中诱导型共刺激因子 B7 同源蛋白共刺激途径的参与”。

Hagiyama H, Kohsaka H et al.: "Two cases of acute respiratory distress syndrome resulting from adult onset Still's disease"Mod Rheumatol. 13. 76-80 (2003)

Hagiyama H、Kohsaka H 等人：“两例成人发病的斯蒂尔病导致的急性呼吸窘迫综合征”Mod Rheumatol。

Positional effect of amino acid replacement on peptide antigens for the increased IFN-gamma production from CD4 T cells.

氨基酸置换对肽抗原的位置影响，以增加 CD4 T 细胞的 IFN-γ 产量。

• 发表时间: 2005
• 期刊: Allergol Int 54(1)
• 作者: Liu T;Kohsaka H;Suzuki M;Takagi R;Hashimoto K;Uemura Y;Ohyama H;Matsushita S
• 通讯作者: Matsushita S

Nonomura Y, Kohsaka H. et al.: "Gene transfer of a cell cycle modulator exerts anti-inflammatory effects in the treatment of arthritis"J Immunol. 171(9). 4913-4919 (2003)

Nonomura Y、Kohsaka H. 等人：“细胞周期调节剂的基因转移在关节炎的治疗中发挥抗炎作用”J 免疫学杂志。

Nishio J, Kohsaka H et al.: "Development of TCRB CDR3 length repertoire of human lymphocytes."Int Immunol. 16(3). 423-431 (2003)

Nishio J、Kohsaka H 等人：“人类淋巴细胞 TCRB CDR3 长度库的开发”。