Molecular Maternal Effect on Etiology of cleft lip nad palate in Fetal Mouse Model.

分子母体对胎鼠模型唇裂和腭裂病因的影响。

• 批准号: 15390638
• 负责人: NONAKA Kazuaki
• 金额: $ 3.14万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390638/
• 关键词: Maternal Effect; Amnio Fluid; Cleft lip; Cleft Palate; CL; Fv Mouse; Fusion; Molecular Event; TGF-beta3; 口唇裂口蓋裂自然発症CL; Frマウス; 血管新生; 組織癒合; 歯の発育; 頚顔面頭蓋発育; 口唇裂; 口唇癒合; CL; TGF-β3; 分子機序

TGF-b3 mediates epithelial-mesenchymal transformation during normal fusion of lip and palate, but how TGF-b3 functions during cleft lip repair remains unexplored. We hypothesize that TGF-b3 promotes fetal cleft lip repair and fusion by increasing the availability of nesenchymal cells. In this investigation, we demonstrated that cleft lips in mouse fetuses were repaired by fetal surgery, producing scarless fusion. At the site of the operation, we first observed an infusion of platelet expressing TGF-b3, followed by increased expression of cyclin D1 and tenascin-C, and coupled with increased mesenchymal cell proliferation. In an ex vivo serumless culture system, cleft lip explants fused in the presence of exogenous TGF-b3. Cultured lips also showed up-regulation in cyclin D1 and tenascin-C expression. These findings suggest that microsurgical repair of cleft lip in the fetus that produced scarless fusion is mediated by TGF-b3 regulation of mesenchymal cell proliferation and migration at the site of repair.

TGF-β 3介导的上皮间充质转化在正常的唇腭裂融合，但TGF-β 3如何在唇裂修复功能仍然是未知的。我们推测TGF-β 3通过增加间充质细胞的可用性促进胎儿唇裂修复和融合。在这项研究中，我们证明了小鼠胎儿唇裂可以通过胎儿手术修复，产生无瘢痕融合。在手术部位，我们首先观察到表达TGF-β 3的血小板输注，随后细胞周期蛋白D1和生腱蛋白C的表达增加，并伴有间充质细胞增殖增加。在体外无血清培养系统中，唇裂外植体在外源性TGF-β 3的存在下融合。培养的嘴唇也表现出上调细胞周期蛋白D1和tenascin-C的表达。这些研究结果表明，显微手术修复胎儿唇裂，产生无瘢痕融合介导的TGF-β 3调节间充质细胞的增殖和迁移的网站的修复。

项目成果

TGF-beta-3 promotes scarless repair of cleft lip in mouse fetuses

• DOI: 10.1177/154405910208101007
• 发表时间: 2002-10-01
• 期刊: JOURNAL OF DENTAL RESEARCH
• 影响因子: 7.6
• 作者: Kohama, K;Nonaka, K;Ohishi, M
• 通讯作者: Ohishi, M

TGF-beta-3 Induces Dentin-like Tissue Formation in Dental Pulpal Tissue of Fetal Mouse Tooth Germ

TGF-β-3 诱导胎鼠牙胚牙髓组织中牙本质样组织的形成

• 发表时间: 2005
• 期刊: Growth, Development and Differentiation (印刷中)
• 作者: Muhetaer H;Muraoka N;Fukumoto S;Nakashima M;Akamine F;K.Nonaka;et al.
• 通讯作者: et al.

TGF-beta-3 Induces Dentin-like Tissue Formation in Dental Pulpal Tissue of Fetal Mouse Tooth Germ.

TGF-β-3 诱导胎鼠牙胚牙髓组织中牙本质样组织的形成。

• 发表时间: 2005
• 期刊: Develop. Growth Differ. 47
• 作者: H.Muhetaer;N.Muraoka;R.Hosokawa;M.Nakashima;F.Akamine;K.Nonaka;M.Ohishi
• 通讯作者: M.Ohishi

R.Hosokawa, K.Nonaka, M.Morifuji, L.Shum, M.Ohishi: "TGF-β3 Decreases Type I Collagen and Scarring after Labioplasty"Journal of Dental Research. 82・7. 558-564 (2003)

R.Hosokawa、K.Nonaka、M.Morifuji、L.Shum、M.Ohishi：“TGF-β3 减少阴唇成形术后的 I 型胶原蛋白和疤痕”《牙科研究杂志》82・7 (2003)。

TGF-beta3 decrease type I collagen and scarring after labioplasty.

TGF-β3 可减少阴唇整形术后 I 型胶原蛋白和疤痕形成。

• 发表时间: 2003
• 期刊: J.Dent.Res. 82(7)
• 作者: Hosokawa R^*.;Nonaka K^*.;Morifuji M.;Shum L.;Ohishi M.
• 通讯作者: Ohishi M.