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Biotechnological study to know the effects of genetic and matemal factors on craniofacial growth in mice.

生物技术研究了解遗传和物质因素对小鼠颅面生长的影响。

基本信息

批准号：
02807188
负责人：
NONAKA Kazuaki
金额：
$ 1.02万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807188/
关键词：
Mouse Embryo Development in vitro Heritability Prenatal Maternal Effect Craniofacial Development Embryo Transfer Cephalometry Image Scanning System Prenatal maternal effect Craniofacial development Embryo transfer craniofacil develop

项目摘要

To examine the degree of the relative contribution of genetic and environmental effects to preimplantation development of mouse embryo in vitro, two-cell stage embryos collected from oviducts of five strains of inbred mice(DDD/QDJ, C3H/ QDJ, C57BL/6J Sea,DBA/1J Sea, and BALB/C Sea) were cultivated up to the stage of expanded blasytocyst for seventy two hours. Both fertility and survival rates were calculated in each recipient and also in each strain. A Student-Newman-Keuls test for both rates showed a significant interstrain difference and an analysis of variance done to estimate the relative importance of genetic and environmental effects on the preimplantation development of embryo showed a low degree of genetic determination for both rates. It was thus possible to conclude that the environmental effect played a more important role on preimplantation development of mouse embryo in vitro.Embryo transfer effect and intra-uterine effect of dam on prenatal development of craniofacial com … More plex of mice embryos were investigated with use of embryo transfer and cephalostat. DDD strain embryos were transferred to the three strains of recipients (DDD, C57BL and DBA). The cephalometric observation of newborn offspring developed from transferred embryos was performed just after parturition. Dorso-ventral craniofacial size of newborn offspring was calculated using values of X-and Y-coordinates on dorso-ventral cephalogram. Statistical analysis showed that a significant inter-group difference in craniofacial size between transferred and non-transferred groups as well as a significant inter-strain difference among those of the three strains of recipients were observed. Thus, it was disclosed that embryo transfer technique might retard the prenatal development of craniofacial complex of transferred embryo and that the three strains of recipients contributed unequally to the prenatal development of craniofacial complex of transferred embryo through each of their intra-uterine environment as a prenatal matemal effect. These results indicated that the intra-uterine environment of recipient played an important role in the prenatal development of craniofacial complex of mice embryo. Less

为探讨遗传和环境效应对小鼠胚胎体外着床前发育的相对影响程度，从5个近交系小鼠（DDD/QDJ、C3 H/ QDJ、C57 BL/6 J Sea、DBA/1 J Sea和BALB/C Sea）输卵管中采集2细胞期胚胎，培养至囊胚期。计算每个受体和每个品系的生育率和存活率。两种速率的Student-Newman-Keuls检验均显示出显著的品系间差异，估计遗传和环境效应对胚胎着床前发育的相对重要性的方差分析显示，两种速率的遗传决定程度较低。因此，环境因素对小鼠胚胎体外着床前发育的影响更为重要。胚胎移植及宫内母体对颅面角发育的影响 ...更多信息采用胚胎移植和头颅固定器对小鼠胚胎的发育进行了观察。将DDD品系胚胎移植到DDD、C57 BL和DBA三个品系受体中。分娩后立即对移植胚胎发育的新生儿进行头影测量观察。利用X-和Y-坐标值计算新生儿后代的背腹颅面大小。统计分析表明，转移组和非转移组的颅面尺寸存在显着的组间差异，以及三个品系受体之间的颅面尺寸存在显着的品系差异。因此，揭示了胚胎移植技术可能延迟移植胚胎的颅面复合体的产前发育，并且三种受体品系通过其各自的子宫内环境作为产前母体效应对移植胚胎的颅面复合体的产前发育的贡献是不相等的。这些结果表明，受体子宫内环境对小鼠胚胎颅面复合体的产前发育起重要作用。少