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The animal experimental study to examine the availability of fetus surgery in CL/Fr mouse feutses affected with cleft lip and palate

动物实验研究探讨唇裂和腭裂 CL/Fr 小鼠胎儿接受胎儿手术的可行性

基本信息

批准号：
07557135
负责人：
NONAKA Kazuaki
金额：
$ 10.69万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Purpose : There is a deficiency of a study of wound healing in fetal mice or rats. The present study has been undertaken to examine whether or not fetal healing on surgical suture of clefted lip in CL/Fr mouse fetus affected with cleft lip can occur without an apparent inflammatory response and take place with the deposition of collagen.Material and Method : The experimental model chosen for this study was a through wound of the upper lip, and the animals were followed for 72 hours after surgery. The-mated adult female CL/Fr mouse of ten to fifteen weeks old were anesthetized with enthoren on the 15th day, 16th or 17th day of gestation. A laporotomy was performed in each animal to gain access to the pregnant uterus. Each experimental fetus was partially exteriorized through a small hysterotomy and a through and through surgical wound made in the upper lip approximately 2 mm from the mid line. Each fetus was returned to the lumen of the uterus, and the hysterotomy closed with a purse st … More ring suture. The laparotomy wounds were closed in layrs and the animals allowed to recover from the anesthetic. The pregnancies then continued nntil the fetuses were harvested. The injured fetuses were divided into the following three groups, each containing ten or eleven animals. In the first group, the wounds made on the normal lip of the unaffected fetuses were sutured. In the second group, the clefted lip of the affected fetuses were only sutured wothout wound, while in the third group, the wounds made on the clefted lip of the affected fetuses were sutured using 11/0 nylon and microsurgical techniques. In each group, ten or eleven fetuses were harvested at 24 hours, 48 hours or 72 hours after wounding. Each harvested fetus was fixed in formal saline, and haematoxylin and eosin stained horizontal sections of the upper lip were prepared.Result : A total of 32 fetal mice could be successfully obtained through the fetus surgery experiment of a wound of the upper lip. In each group, four or five fetuses harvested at 72 hours after wounding were subjected to a histological examination. The following results were obtained.1) The mesenchymal elements were connecting each other from both wounded sites in three out of four fetuses for the first group.2) The mesenchymal elements were connecting each other from both wounded sites in only one out of five fetuses for the second group.3) The mesenchymal elements were connecting each other from both wounded sites in two out of five fetuses for the third group.These results suggested the useful possibility for the mesenchymal tissue to regenerate from both incised site of the clefted lip in CL/Fr mouse fetuses. Less

目的：目前对胎鼠伤口愈合的研究还存在不足.本研究已经进行了检查是否胎儿愈合CL/Fr小鼠胎儿唇裂的影响与唇裂的手术缝合唇可以发生没有明显的炎症反应，并发生与沉积的collagen.Material和方法：本研究选择的实验模型是通过伤口的上唇，动物手术后72小时进行了跟踪。将10 ~ 15周龄的雌性CL/Fr小鼠在妊娠第15、16或17天处死，取其卵母细胞进行麻醉。对每只动物进行剖腹术，以进入妊娠子宫。通过小子宫切开术和在上唇距中线约2 mm处制作的贯穿手术伤口，将每只实验胎仔部分取出。每个胎儿都被放回子宫腔，子宫切口用一个荷包缝合， ...更多信息环形缝合。将剖腹手术伤口分层闭合，并使动物从麻醉中恢复。然后继续妊娠，直到收获胎儿。将损伤胎仔分为以下3组，每组10只或11只动物。第一组缝合正常胎仔唇缘伤口。第二组仅缝合患侧唇，不缝合伤口;第三组用11/0尼龙线缝合患侧唇上的伤口，并采用显微外科技术。每组分别于伤后24 h、48 h、72 h取胎10 ~ 11只。结果：通过胎鼠上唇创伤手术实验，共获得胎鼠32只，其中12只胎鼠的上唇创伤手术成功，其余32只胎鼠的上唇创伤手术成功，其余32只胎鼠的上唇创伤手术成功。在每组中，在创伤后72小时收获的四个或五个胎儿进行组织学检查。结果表明：1）第一组中有3/4的胎儿在两个损伤部位的间充质细胞相互连接; 2）第二组中只有1/5的胎儿在两个损伤部位的间充质细胞相互连接。第三组5个胎儿中有2个胎儿的间充质成分从两个损伤部位相互连接。间充质组织从CL/Fr小鼠胎儿的唇裂的两个切开部位再生。少