Basic and clinical research on recovery of vision by electrical stimulation

电刺激恢复视力的基础与临床研究

• 批准号: 22249058
• 负责人: FUJIKADO Takashi
• 金额: $ 30.7万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22249058/
• 关键词: 神経保護; 電気刺激; 人工視覚; 網膜機能画像; ラマン分光; 網膜機能イメージング

IGF1 mRNA was increased by the electrical stimulation on cultured RGC5, which suggests that IGF1 is involved in the neuro-protection mechanism by electrical stimulation (ES). The process of neuronal cell-death was demonstrated sequentially and 2-dimensionally by Raman microscope by probing the intracellular changes of cytochrome C. Trans-corneal electrical stimulation (TES) on rabbit model of retinal degeneration showed a decrease of OPL thickness, which suggests the neuro-protective effect of ES on photoreceptors. Clinical study on the artificial vision by optic nerve stimulation showed the efficacy of ES to elicit phosphine for 2 years for advanced retinitis pigmentosa patients.

电刺激可使培养的RGC5细胞IGF1 mRNA表达增加，提示IGF1参与了电刺激的神经保护机制。利用拉曼显微镜观察细胞色素C在细胞内的动态变化，从二维的角度对神经细胞死亡过程进行了连续的观察。经角膜电刺激（TES）可使兔视网膜退变模型的OPL厚度减少，提示ES对光感受器有神经保护作用。视神经刺激人工视觉的临床研究表明，ES激发磷化氢2年对晚期视网膜色素变性患者的疗效。

项目成果

Automated processing of label-free Raman microscope images of macrophage cells with standardized regression for high-throughput analysis

通过标准化回归自动处理巨噬细胞的无标记拉曼显微镜图像以进行高通量分析

• 发表时间: 2010
• 期刊: Immunome Res.
• 作者: Milewski RJ;Kumagai Y;Fujita K;Standley DM;Smith NI.
• 通讯作者: Smith NI.

Activated Protein C Rescues the Retina from Ischemia-Induced Cell Death

活化的蛋白 C 可将视网膜从缺血引起的细胞死亡中拯救出来。

• DOI: 10.1167/iovs.10-5557
• 发表时间: 2011-02-01
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Du, Zhao-Jiang;Yamamoto, Takuhiro;Kamei, Motohiro
• 通讯作者: Kamei, Motohiro

Characteristics of retinal reflectance changes induced by transcorneal electrical stimulation in cat eyes.

• DOI: 10.1371/journal.pone.0092186
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Morimoto T;Kanda H;Miyoshi T;Hirohara Y;Mihashi T;Kitaguchi Y;Nishida K;Fujikado T
• 通讯作者: Fujikado T

Effect of Low‐Additional Soft Contact Lenswith Decentered Optical Design on Myopia Progression inChildren

偏心光学设计低附加值软性隐形眼镜对儿童近视进展的影响

• 发表时间: 2013
• 作者: Morimoto T;Kanda H;Endo T;Nishida K;Fujikado T;不二門尚;Fujiakdo Takashi;Fujikado T;Fujikado Takashi
• 通讯作者: Fujikado Takashi

Feasibility of 2nd Generation STS Retinal Prosthesis with 49 Channel Electrode Array in Dogs

具有 49 通道电极阵列的第二代 STS 视网膜假体在狗中的可行性

• 发表时间: 2013
• 作者: Fujikado T;Kamei M;KishimaH;Maruo T;Osawa K;OzawaM
• 通讯作者: OzawaM