Basic and clinical research on recovery of vision by electrical stimulation

电刺激培养的RGC5后，IGF1基因表达增加，提示IGF1参与了电刺激(ES)的神经保护机制。通过细胞色素C在细胞内的变化，在拉曼显微镜下连续、二维地显示了神经细胞死亡的过程。经角膜电刺激(TES)对兔视网膜变性模型的OPL厚度有明显的降低作用，提示ES对光感受器具有神经保护作用。对视神经刺激人工视觉的临床研究表明，ES诱导磷化氢治疗晚期视网膜色素变性患者2年的疗效。

IGF1 mRNA was increased by the electrical stimulation on cultured RGC5, which suggests that IGF1 is involved in the neuro-protection mechanism by electrical stimulation (ES). The process of neuronal cell-death was demonstrated sequentially and 2-dimensionally by Raman microscope by probing the intracellular changes of cytochrome C. Trans-corneal electrical stimulation (TES) on rabbit model of retinal degeneration showed a decrease of OPL thickness, which suggests the neuro-protective effect of ES on photoreceptors. Clinical study on the artificial vision by optic nerve stimulation showed the efficacy of ES to elicit phosphine for 2 years for advanced retinitis pigmentosa patients.