Development of on-demand multifunctional delivery systems using dendron lipid assemblies

我们开发了多种类型的功能性树突状细胞脂类，包括基因传递树突状细胞脂类、温度敏感树突状细胞脂、pH敏感树突状细胞脂和磁共振成像树突状细胞脂。我们发现，通过疏水相互作用和质子海绵效应，基因传递树枝状脂类实现了高效的基因导入培养细胞。温度-树枝状脂类被证明提供了一种新型的温度响应性囊泡，在特定温度下经历形态转变。对pH敏感的树枝状脂类与蛋黄磷脂酰胆碱形成对pH高度敏感的囊泡，释放细胞内体中的内容。磁共振成像树突状脂类有助于检测脂质体的生物分布和蓄积，从而指导个体化化疗。树枝状脂质技术有望为开发新型高效的非侵入性肿瘤治疗方法做出贡献。

We developed various types of functional dendron-bearing lipids, which include gene delivery dendron lipids, temperature-sensitive dendron lipids, pH-sensitive dendron lipids, and MR imaging dendron lipids. We found that gene delivery dendron lipids achieved highly efficient gene transfection into culture cells through hydrophobic interaction and proton sponge effect. Temperature-dendron lipids were shown to provide a new type of temperature-responsive vesicles that undergo morphology transition at a specific temperature. pH-Sensitive dendron lipids formed highly pH-responsive vesicles with egg yolk phosphatidylcholine that release contents in the endosome of a cell. MR imaging dendron lipids were useful for detection of bio-distribution and accumulation of liposomes, which can lead to personalized chemotherapy. The dendron lipid technology is expected to contribute development of new efficient noninvasive cancer therapy.