Development of on-demand multifunctional delivery systems using dendron lipid assemblies

使用树突脂质组件开发按需多功能递送系统

• 批准号: 23240075
• 负责人: KENJI Kono
• 金额: $ 31.95万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23240075/
• 关键词: 薬物伝達システム; ナノ医療; 薬物送達システム; がん治療; ナノバイオ; 薬物送達; イメージング; 自己組織化; ワクチン

We developed various types of functional dendron-bearing lipids, which include gene delivery dendron lipids, temperature-sensitive dendron lipids, pH-sensitive dendron lipids, and MR imaging dendron lipids. We found that gene delivery dendron lipids achieved highly efficient gene transfection into culture cells through hydrophobic interaction and proton sponge effect. Temperature-dendron lipids were shown to provide a new type of temperature-responsive vesicles that undergo morphology transition at a specific temperature. pH-Sensitive dendron lipids formed highly pH-responsive vesicles with egg yolk phosphatidylcholine that release contents in the endosome of a cell. MR imaging dendron lipids were useful for detection of bio-distribution and accumulation of liposomes, which can lead to personalized chemotherapy. The dendron lipid technology is expected to contribute development of new efficient noninvasive cancer therapy.

我们开发了多种类型的功能性树突状细胞脂类，包括基因传递树突状细胞脂类、温度敏感树突状细胞脂、pH敏感树突状细胞脂和磁共振成像树突状细胞脂。我们发现，通过疏水相互作用和质子海绵效应，基因传递树枝状脂类实现了高效的基因导入培养细胞。温度-树枝状脂类被证明提供了一种新型的温度响应性囊泡，在特定温度下经历形态转变。对pH敏感的树枝状脂类与蛋黄磷脂酰胆碱形成对pH高度敏感的囊泡，释放细胞内体中的内容。磁共振成像树突状脂类有助于检测脂质体的生物分布和蓄积，从而指导个体化化疗。树枝状脂质技术有望为开发新型高效的非侵入性肿瘤治疗方法做出贡献。

项目成果

Multi-functional liposomes having temperature-triggered release and magnetic resonance imaging for tumor-specific chemotherapy

• DOI: 10.1016/j.biomaterials.2010.10.050
• 发表时间: 2011-02-01
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Kono, Kenji;Nakashima, Seiji;Ishizaka, Yukihito
• 通讯作者: Ishizaka, Yukihito

Dendron lipids for preparation of functional carrier systems

用于制备功能性载体系统的Dendron脂质

• 发表时间: 2013
• 作者: Iwata;T;K. Kono
• 通讯作者: K. Kono

Development of Stimuli-Responsive Liposomes for Biomedical Applications.

用于生物医学应用的刺激响应脂质体的开发。

• 发表时间: 2013
• 作者: Ogata;Y;Kenji Kono
• 通讯作者: Kenji Kono

ポリアミドアミンデンドロン脂質ベクター：分子構造の影響

聚酰胺胺树枝状脂质载体：分子结构的影响

• 发表时间: 2012
• 作者: 塚本康太;弓場英司;原田敦史;河野健司
• 通讯作者: 河野健司

ポリアミドアミンデンドロン脂質を用いる温度応答性キャリアの構築

使用聚酰胺胺树枝状脂质构建温度响应性载体

• 发表时间: 2011
• 作者: 平中勇気;弓場英司;原田敦史;河野健司
• 通讯作者: 河野健司