denitrifying factory

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Adequate management of groundwater is strongly required against increasing threat of water shortage. Nitrate contamination is remained problems for groundwater. To minimize nitrate contamination we surveyed nitrate concentration and estimated in situ denitrification activity of groundwater by stable isotope tracer experiment. And we revealed corresponding microbial populations using gene analysis with qPCR targeting denitrification functional genes. Constraints of denitrification are also elucidated. Acetate showed the highest concentration among the measured organic compounds dissolved in the groundwater. And when the groundwater was incubated with 15NO3- by adding acetate debitirification activity increased about twice compared with the incubation under the in situ condition. And soil microbes around the wells enhanced denitrification about three times. Basic understandings were thus obtained to construct in situ highly active denitrification system for the groundwater.