denitrifying factory

为了应对日益严重的缺水威胁，迫切需要对地下水进行适当的管理。地下水硝酸盐污染问题一直存在。为了减少硝酸盐污染，我们通过稳定同位素示踪试验测量了地下水的硝酸盐浓度，并估计了地下水的原位反硝化活性。利用qPCR技术对反硝化功能基因进行基因分析，揭示了相应的微生物种群。对反硝化的制约条件也进行了阐述。在地下水中溶解的有机化合物中，乙酸盐的浓度最高。添加乙酸15NO3-孵育后，地下水的脱氮活性比原地孵育时提高了约2倍。水井周围的土壤微生物将反硝化作用提高了三倍。从而对构建地下水原位高效脱氮系统有了基本认识。

Adequate management of groundwater is strongly required against increasing threat of water shortage. Nitrate contamination is remained problems for groundwater. To minimize nitrate contamination we surveyed nitrate concentration and estimated in situ denitrification activity of groundwater by stable isotope tracer experiment. And we revealed corresponding microbial populations using gene analysis with qPCR targeting denitrification functional genes. Constraints of denitrification are also elucidated. Acetate showed the highest concentration among the measured organic compounds dissolved in the groundwater. And when the groundwater was incubated with 15NO3- by adding acetate debitirification activity increased about twice compared with the incubation under the in situ condition. And soil microbes around the wells enhanced denitrification about three times. Basic understandings were thus obtained to construct in situ highly active denitrification system for the groundwater.