High sensitivity analysis of microorganisms in aquatic environment using image analyzer

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Estimate of microbial biomass in aquatic environment could be a bottle neck in discussion of carbon balance on earth, because it becomes to be known generally that planktonic microbes predominate in waters. Thus we tried to estimate these biovolume precisely using image analysis. Bacteria was selected as a model microbes and various cellular parameters were measured under epifluorescence microscopy. It was confirmed that DAPI stains clearly chromosomal DNA and acridine orange stains single strand RNA (result will be published from Plenum Publishing Corp.in "Microbial Diversity in Time and Space").The changes in cellular sizes were explained with their growth rate and growth phase. It was confirmed that bacteria became small in stationary phase loosing their constituting single strand RNA and cellular morphological change occurred longitudinally. We tried to explain the varying viability of bacteria determined by plate counting from bacterial distribution in cell cycle. The percent plate counting bacteria to total was within the number of the cells distributed in C- and D-period. Cells in I-period are not considered to form colony easily (result is submitted).