High sensitivity analysis of microorganisms in aquatic environment using image analyzer

使用图像分析仪对水生环境中的微生物进行高灵敏度分析

• 批准号: 05680445
• 负责人: KATO Kenji
• 金额: $ 1.28万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680445/
• 关键词: Aquatic environment; microbes; biovolume; cellular sizes; image analysis; phase-contrast epifluorescence microscopy; バクテリア; プランクトン; 物質循環; 顕微鏡画像解析; 水圏; 炭素収支; 植物プランクトン; 地球環境

Estimate of microbial biomass in aquatic environment could be a bottle neck in discussion of carbon balance on earth, because it becomes to be known generally that planktonic microbes predominate in waters. Thus we tried to estimate these biovolume precisely using image analysis. Bacteria was selected as a model microbes and various cellular parameters were measured under epifluorescence microscopy. It was confirmed that DAPI stains clearly chromosomal DNA and acridine orange stains single strand RNA (result will be published from Plenum Publishing Corp.in "Microbial Diversity in Time and Space").The changes in cellular sizes were explained with their growth rate and growth phase. It was confirmed that bacteria became small in stationary phase loosing their constituting single strand RNA and cellular morphological change occurred longitudinally. We tried to explain the varying viability of bacteria determined by plate counting from bacterial distribution in cell cycle. The percent plate counting bacteria to total was within the number of the cells distributed in C- and D-period. Cells in I-period are not considered to form colony easily (result is submitted).

水环境中微生物生物量的估计可能是讨论地球碳平衡的一个瓶颈，因为人们普遍知道，浮游微生物在沃茨中占主导地位。因此，我们试图使用图像分析来精确地估计这些生物量。选择细菌作为模式微生物，并在落射荧光显微镜下测量各种细胞参数。证实了DAPI清楚地染色染色体DNA和吖啶橙子染色单链RNA（结果将由Plenum Publishing Corp.in“Microbial Diversity in Time and Space”出版）。证实了细菌在稳定期变小，失去其组成的单链RNA，细胞形态发生纵向变化。我们试图从细菌在细胞周期中的分布来解释平板计数所确定的细菌活力的变化。平板计数细菌占总数的百分比在C期和D期分布的细胞数范围内。认为I期细胞不易形成集落（提交结果）。

项目成果

K.Kato: "Microbial Diversity in Time and Space 所収論文標題:Image analysis on the diversiry of bacteral cell sige" Plenum, 250 (1995)

K.Kato：“时间和空间中的微生物多样性包括论文标题：细菌细胞多样性的图像分析”Plenum，250（1995）

Kato, K.(one chapter): Microbial Diversity in Time and Space. Plenum Publishing Corporation, (1995)

Kato, K.（一章）：时空微生物多样性。

Kato,K.& T.Someya: "Multiple analyses on bacterial growth and culturability-a case study for Escherichia coli." Applied and Environmental Microbiology. (submitted).

加藤，K.

Kato,K.(one chapter): "Microbial Diversity in Time and Space" Plenum Publishing Corporation, (1995)

Kato,K.（一章）：“时间和空间中的微生物多样性”Plenum Publishing Corporation，（1995）

Kato, K.& T.Someya: "Multiple analyzes on bacterial growth and culturability-a case study for Escherichia coli." Applied and Environmental Microbiology. (submitted).

加藤，K.