Impact of green house effect on marine bacteria with 5C

5C下温室效应对海洋细菌的影响

• 批准号: 16310024
• 负责人: KATO Kenji
• 金额: $ 7.42万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310024/
• 关键词: ESTUARY; PLANKTONIC BACTERIA; FLUORESCENCE IN SITU HYBRIDIZATION; TEMPERATURE; SALINITY; PHYTOPLANKTON; DISSOLVED ORGANIC CARBON; 海水温度上昇; 細菌群集構造; 遺伝子解析; コレラ菌; 海水温上昇; 細菌; FISH法; 群集構造; ガンマ・プロテオバクテリア; ビブリオ・コレラ

An intensive study was carried out in Simizu-Port of Suruga Bay throughout 2004 and 2005. Together with the results of preceding observation since 2001, we obtained knowledge about planktonic bacterial abundance and their community structure revealed with fluorescence in situ hybridization (FISH). Fluctuation of these numbers is discussed with environmental change as temperature, salinity, phytoplankton abundance and dissolved organic carbon supplied from primary production and terrestrial activities. In particular the population dynamics is chased targeting Vibrio cholerae using specific gene techniques.When compared the whole data set of bacterial abundance with related environments : 1)Temperature showed positive correlation with total bacterial number (P<0.01) and domain Bacteria (P<0.05). 2)Negative relationship with salinity were found for total bacteria (P<0.01), domain Bacteria (P<0.01), gamma -Proteobacteria (P<0.01) and domain Achaea (P<0.05). 3)Abundance of phytoplankton represented by Chl.a showed a higher correlation with domain Bacteria (P<0.01) and that of somewhat lower correlation with gamma-Proteobacteria was then total bacterial number (P<0.05).These findings give us an overview that planktonic bacterial abundance in studied coastal area was governed by fresh water supplied from rivers and it was affected by the shift of temperature though this didn't affect Archaeal abundance. Phytoplankton was then another regulator of bacterial abundance.Vibrio cholerae was chosen a target bacteria which responds to temperature sensitively being a marked harmful bacteria. Method to chase population of cholerae was successfully established, applicable for the studied environment.

2004年和2005年期间，在骏河湾的清水港进行了深入的研究。结合2001年以来的观测结果，我们利用荧光原位杂交（FISH）技术对浮游细菌的丰度及其群落结构进行了研究。这些数字的波动进行了讨论与环境变化的温度，盐度，浮游植物丰度和溶解有机碳提供初级生产和陆地活动。将细菌丰度数据集与相关环境进行比较发现：1）温度与细菌总数（P<0.01）和结构域细菌（P<0.05）呈正相关; 2)细菌总数（P<0.01）、结构域细菌（P <0.01）、γ-变形菌（P<0.01）和结构域Achaea（P<0.05）与盐度呈负相关. 3)以Chl. a为代表的浮游植物丰度与结构域细菌丰度相关性较高与γ-变形菌相关性较低的是细菌总数（P<0.05）。这些结果表明，研究海域浮游细菌的丰度受河流淡水供应的控制，受温度变化的影响，但温度变化对浮游细菌的丰度没有影响。丰富的食物。浮游植物是细菌丰度的另一个调节器，霍乱弧菌是一种对温度敏感的有害细菌，被选为目标细菌。成功地建立了适用于所研究环境的霍乱种群追踪方法。