Impact of green house effect on marine bacteria with 5C

在2004年和2005年期间，在骏河湾的simizu港进行了一项深入研究。结合2001年以来的观测结果，利用荧光原位杂交技术（fluorescence in situ hybridization， FISH）对浮游生物的细菌丰度及其群落结构进行了研究。讨论了这些数字的波动与温度、盐度、浮游植物丰度、初级生产和陆地活动提供的溶解有机碳等环境变化的关系。特别是利用特定基因技术追踪霍乱弧菌的种群动态。结果表明：1)温度与细菌总数（P<0.01）、区域细菌数（P<0.05）呈显著正相关；2) total bacteria （P<0.01）、domain bacteria （P<0.01）、gamma -Proteobacteria （P<0.01）和domain Achaea （P<0.05）与盐度呈负相关。3)以Chl为代表的浮游植物丰度。a与domain Bacteria的相关性较高（P<0.01），与gamma-Proteobacteria的相关性较低（P<0.05）。这些发现为我们概述了研究区浮游细菌丰度受河流淡水供应支配，并受到温度变化的影响，但古细菌丰度不受温度变化的影响。当时，浮游植物是细菌数量的另一个调节器。霍乱弧菌作为一种显著的有害细菌，对温度反应敏感。成功建立了霍乱种群追踪方法，适用于所研究的环境。

An intensive study was carried out in Simizu-Port of Suruga Bay throughout 2004 and 2005. Together with the results of preceding observation since 2001, we obtained knowledge about planktonic bacterial abundance and their community structure revealed with fluorescence in situ hybridization (FISH). Fluctuation of these numbers is discussed with environmental change as temperature, salinity, phytoplankton abundance and dissolved organic carbon supplied from primary production and terrestrial activities. In particular the population dynamics is chased targeting Vibrio cholerae using specific gene techniques.When compared the whole data set of bacterial abundance with related environments : 1)Temperature showed positive correlation with total bacterial number (P<0.01) and domain Bacteria (P<0.05). 2)Negative relationship with salinity were found for total bacteria (P<0.01), domain Bacteria (P<0.01), gamma -Proteobacteria (P<0.01) and domain Achaea (P<0.05). 3)Abundance of phytoplankton represented by Chl.a showed a higher correlation with domain Bacteria (P<0.01) and that of somewhat lower correlation with gamma-Proteobacteria was then total bacterial number (P<0.05).These findings give us an overview that planktonic bacterial abundance in studied coastal area was governed by fresh water supplied from rivers and it was affected by the shift of temperature though this didn't affect Archaeal abundance. Phytoplankton was then another regulator of bacterial abundance.Vibrio cholerae was chosen a target bacteria which responds to temperature sensitively being a marked harmful bacteria. Method to chase population of cholerae was successfully established, applicable for the studied environment.