Impact of green house effect on marine bacteria with 5C

2004年和2005年，在苏鲁加湾的西水港进行了一项密集的研究。结合2001年以来的前期观测结果，我们获得了利用荧光原位杂交(FISH)揭示浮游细菌丰度及其群落结构的知识。随着环境的变化，如温度、盐度、浮游植物丰度和初级生产和陆地活动提供的溶解有机碳，这些数字的波动被讨论。利用特定基因技术追踪霍乱弧菌的种群动态。当细菌丰度的整个数据集与相关环境进行比较时：1)温度与细菌总数(P&lt；0.01)和区域细菌(P&lt；0.05)呈正相关。2)总细菌(P&lt；0.01)、领域细菌(P&lt；0.01)、伽马变形杆菌(P&lt；0.01)和领域Achaea(P&lt；0.05)与盐度呈负相关。3)以Chl.a为代表的浮游植物丰度与区域细菌的相关性较高(P&lt；0.01)，与伽马变形杆菌的相关性较低(P&lt；0.05)。这些发现表明，研究海域浮游细菌的丰度受河流补给淡水的控制，并受温度变化的影响，但这并不影响古生物的丰度。浮游植物是细菌数量的另一个调节因素。霍乱弧菌被选为对温度敏感的目标细菌，是一种显着的有害细菌。建立了一种适用于本研究环境的霍乱菌群追踪方法。

An intensive study was carried out in Simizu-Port of Suruga Bay throughout 2004 and 2005. Together with the results of preceding observation since 2001, we obtained knowledge about planktonic bacterial abundance and their community structure revealed with fluorescence in situ hybridization (FISH). Fluctuation of these numbers is discussed with environmental change as temperature, salinity, phytoplankton abundance and dissolved organic carbon supplied from primary production and terrestrial activities. In particular the population dynamics is chased targeting Vibrio cholerae using specific gene techniques.When compared the whole data set of bacterial abundance with related environments : 1)Temperature showed positive correlation with total bacterial number (P<0.01) and domain Bacteria (P<0.05). 2)Negative relationship with salinity were found for total bacteria (P<0.01), domain Bacteria (P<0.01), gamma -Proteobacteria (P<0.01) and domain Achaea (P<0.05). 3)Abundance of phytoplankton represented by Chl.a showed a higher correlation with domain Bacteria (P<0.01) and that of somewhat lower correlation with gamma-Proteobacteria was then total bacterial number (P<0.05).These findings give us an overview that planktonic bacterial abundance in studied coastal area was governed by fresh water supplied from rivers and it was affected by the shift of temperature though this didn't affect Archaeal abundance. Phytoplankton was then another regulator of bacterial abundance.Vibrio cholerae was chosen a target bacteria which responds to temperature sensitively being a marked harmful bacteria. Method to chase population of cholerae was successfully established, applicable for the studied environment.