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Genetic analysis of Vrn genes and establishment of their DNA markers in wheat by using Single Nucleotide Polymorphisms (SNPs)

利用单核苷酸多态性 (SNP) 对小麦 Vrn 基因进行遗传分析并建立其 DNA 标记

基本信息

批准号：
13660009
负责人：
KATO Kenji
金额：
$ 2.24万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660009/
关键词：
Wheat Vernalization DNA marker SSR dCAPS AFLP マイクロサテライト日長反応性

项目摘要

Molecular genetic study has been conducted in the present study, and succeeded to establish molecular markers for the selection of four genes for vernalization response, Vrn-A1,Vrn2,Vrn-D1,Vrn4. The detail is given below for each gene.Vrn-A1 ; We could confirm the linkage (12.8cM) with RFLP locus Xwg908,at which CAPS marker was established.Vrn2 ; Linkage with a dCAPS marker of Xwg644-5B and SSR markers (Xgwm408 and Xgwm604) was detected, and four loci could be mapped as follows ; Vrn2-1.6cM-Xwg644-2.5cM-Xgwm408-13.8cM-Xgwm604. As a result, Vrn2 was located on chromosome 5B and we proposed to rename Vrn2 as Vrn-B1. Xgwm408 can be utilized as a practical selection marker of Vrn-B1, since it is closely linked with Vrn-B1 and clear polymorphism can be detected among the Japanese wheat cultivars and the foreign cultivars carrying Vrn-B1.Vrn-D1 ; Vrn-D1 is the most important gene for wheat breeding in Japan, since most of the wheat cultivars with spring growth habit carry this gene. Two SSR … More markers (Xgwm212 and Xgwm292) proved to link with Vrn-D1, at a distance of 4.6cM and 7.4cM, respectively. Although Xgwm292 was rather distantly located, it was highly polymorphic even in the Japanese early maturing cultivars. Therefore, this SSR marker can be utilized as a practical selection marker for the breeding of early maturing cultivars with spring or winter growth habit.Vrn4 ; Several SSR markers, such as Xgwm190,Xgdm3 and Xgwm292 located on 5D chromosome, were analyzed and close linkage (4.5cM) was detected among Vrn4 and Xgdm3. Independence between Vrn4 and Xgwm292, which is closely linked to Vrn-D1, was also confirmed. It was therefore concluded that Vrn4 is located near the centromere on the long arm of 5D chromosome and independent of Vrn-D1. Vrn4 proved to be a new class of vernalization response gene which was different from Vrn-1 orthologue gene, and we proposed to designate as Vrn-D5.Vernalization response genes causing the difference in the degree of winter growth habit were analyzed in DH populations derived from a cross between two winter type cultivars, Winter-type Abukumawase and Chihokukomugi, by using AFLP markers. As a result, three QTL was detected and one QTL was located on the short arm of 7D chromosome. The effect of Vrn-D1 was also suggested. Less

本研究对春化反应基因Vrn-A1、Vrn 2、Vrn-D1、Vrn 4进行了分子遗传学研究，成功地建立了春化反应基因Vrn-A1、Vrn 2、Vrn-D1、Vrn 4的分子标记。每个基因的详细信息如下所示。Vrn-A1 ;我们可以确认连锁Vrn 2：利用一个dCAPS标记Xwg 644 - 5 B与SSR标记进行连锁结果表明，在Xgwm 408和Xgwm 604两个基因型中，有4个位点可以定位：Vrn 2 -1. 6cM-Xgwm 644 -2. 5cM-Xgwm 408 -13. 8 cM-Xgwm 604。因此，Vrn 2位于5 B染色体上，我们建议将Vrn 2重命名为Vrn-B1。Xgwm 408与Vrn-B1紧密连锁，在日本小麦品种和携带Vrn-B1的国外小麦品种中具有明显的多态性，可作为Vrn-B1的实用选择标记。Vrn-D1基因是日本小麦育种中最重要的基因，多数春性小麦品种都携带该基因。两个SSR ...更多信息标记Xgwm 212和Xgwm 292与Vrn-D1连锁，距离分别为4.6cM和7.4cM。虽然Xgwm 292是相当遥远的位置，它是高度多态性，即使在日本早熟品种。利用位于5D染色体上的SSR标记Xgwm 190、Xgdm 3和Xgwm 292进行分析，发现Vrn 4与Xgdm 3之间存在紧密连锁（4. 5cM）。Vrn 4和Xgwm 292之间的独立性也得到了证实，Xgwm 292与Vrn-D1密切相关。因此，Vrn 4位于5D染色体长臂上的着丝粒附近，并且独立于Vrn-D1。利用AFLP分子标记技术，对冬型品种Abukumawase和Chihokukomugi的DH群体中引起冬性差异的春化反应基因进行了分析。检测到3个QTL，其中1个QTL位于7 D染色体短臂上。同时也提示了Vrn-D1的作用。少