Live-cell Imaging Super-resolution Microscope System

活细胞成像超分辨率显微镜系统

• 批准号: 458673139
• 金额: --
• 依托单位: Heinrich-Heine-Universität Düsseldorf
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/458673139?language=en
• 关键词: Live; cell; Imaging; Super; resolution

Here, we request a super-resolution microscope system based on STED (stimulated emission depletion) technique, which will be mainly used for live-cell STED applications. Therefore, the system should provide STED imaging modalities to reduce the amount of energy of the depletion lasers on the sample, while preserving high optical resolution in x, y and z direction. Since STED is not applicable to plant tissue, the system should provide an alternative option to significantly increase resolution in plant samples. The system will be integrated in the multi-user environment of an imaging core facility. Therefore, the handling of the system should be user-friendly, and the system should offer standard confocal functionality next to the super-resolution options.

在这里，我们要求基于STED（受激发射耗尽）技术的超分辨率显微镜系统，该系统将主要用于活细胞STED应用。因此，该系统应提供STED成像模式以减少样品上的耗尽激光器的能量，同时保持x、y和z方向上的高光学分辨率。由于STED不适用于植物组织，该系统应提供一种替代选择，以显着提高植物样品的分辨率。该系统将被纳入成像核心设施的多用户环境。因此，系统的处理应该是用户友好的，并且系统应该在超分辨率选项旁边提供标准的共焦功能。