ESTABLISHMENT OF NOVEL EXPERIMENTAL SYSTEM(S) FOR EVALUATION OF THE IMMUNE FUNCTION OF FOOD COMPONENTS USING CELLS FROM THE INTESTINAL IMMUNE SYSTEM

1.肠道免疫系统分析。(1)给OVA特异性T细胞受体转基因（TCR-Tg）小鼠喂食OVA饲料可增强固有层淋巴细胞的白细胞介素（IL）-5和IL-6分泌。对这些TCR tg小鼠喂食OVA饮食也导致小肠中的过敏反应，这表明其可用作胃肠道食物过敏的动物模型^* 我们发现，当将派尔集合淋巴结树突状细胞添加到含有OVA、TCR-TG T细胞和B细胞的培养物中时，IgA的分泌被诱导。利用肠道免疫系统细胞检测食物成分的免疫功能：（1）研究了食物中核苷酸对肠道相关淋巴组织反应的影响。添加核苷酸饲料（NT（+）饲料）的BALB/c小鼠T细胞受体（TCR）γ 0上皮内淋巴细胞（IEL）的比例显著高于不添加核苷酸的对照饲料。来自NT（+）饮食喂养的小鼠的肠上皮细胞（IEC）产生更高水平的互锁（IL-）7和TGF-β，其分别在TOR γ δ o ^+ IEL和IgA应答的发展中是重要的，而不是来自对照饮食喂养的小鼠。当将核苷酸添加到小鼠IEC的原代培养物中时，EL-7的分泌增加，但TGF-β的分泌不增加。这些结果表明，核苷酸可以直接改变IEC分泌IL-7的能力，并表明，在饲喂NT（+）饮食的小鼠中，IEC分泌IL-7增加的机制与TGF-β分泌增加的机制不同。(2)口服乙酰柠檬酸三丁酯（ATBC），这是一种用于食品容器中的增塑剂，影响了OVA喂养的TCR-Tg小鼠肠道的组胺含量，表明ATBC改变了肠道中的过敏反应。

1. Analysis of intestinal immune system. (1) Feeding OVA diet to OVA-specific T cell receptor transgenic (TCR-Tg) mice enhanced interleukin (IL)-5 and IL-6 secretion of lamina propria lymphocytes. Feeding of OVA diet to these TCR tg mice also resulted in allergic reactions in the small intestine, suggesting its usefulness as an animal model for gastrointestinal food allergy^* We found that when Peyer's patch dendritic cells were added to cultures containing OVA, TCR-TG T cells and B cells, the secretion of IgA was induced.2. Examination of the immune function of food components using cells from the intestinal immune system, (1) ,Effect of dietary nucleotides on the response of gut-associated lymphoid tissue was investigated. The proportion of T-cell receptor (TCR) γ0 intraepithelial lymphocytes (IEL) in BALB/c mice fed a diet supplemented with nucleotides (NT(+) diet) was significantly higher than that in mice fed the nucleotide-free control diet. Intestinal epithelial cells (IEC) from NT(+) diet-fed mice produced higher levels of interlocking (IL-) 7 and TGF-β, which are important in the development of TORγδo^+ IEL and in IgA responses, respectively, than those from control diet-fed mice. When nucleotides were added to a primary culture of mouse IEC, secretion of EL-7, but not that of TGF-β, was increased. These results demonstrate that nucleotides can modify directly the secretion of IL-7 from IEC, and; suggest that the mechanisms underlying the increased secretion of IL-7 from IEC in mice fed the NT(+) diet were different from those for the increased secretion of TGF-β. (2) Oral administration of acetyl tributyl citrate (ATBC), which is a plasticizer used in food containers, affected the histamine content of the intestine of OVA-fed TCR-Tg mice, suggesting that ATBC modified allegic reactions in the intestine.