Study of the regulatory function for immune and allergic responses by the intestinal microbacteria and probiotic bacteria

肠道微生物和益生菌对免疫和过敏反应调节功能的研究

• 批准号: 16380093
• 负责人: KAMINOGAWA Shuichi
• 金额: $ 10.11万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380093/
• 关键词: Bifidobacterium; Peyer's patch; IFN-γ; IL-6; allergy; パイエル板; IgA; IgE

The association of intestinal bacteria in the gut is considered to contribute to the development and maintaining homeostasis of intestinal immune systems. Functional intestinal microbacteria or probiotics are expected to modulate the immune responses or regulate allergic reactions in the host. However, the immune function of intestinal bacteria on intestinal immune systems has not been clarified. We examined the direct effects of intestinal bacteria on intestinal immune responses to dietary antigen by establishing a gnotobiotic model of ovalbumin (OVA) specific T cell receptor transgenic (OVA-Tg) mice. Using these mice, we examined the effects of the association of Bifidobacterium pseudocatenulatum 7041 (Bp) on immune responses by Peyer's patches (PP), which are inductive sites of the intestinal immune system. Germ-free OVA-Tg mice were associated with (Bif) or without (Cont) Bp and then associated with segmented filamentous bacteria and clostridia. These mice were fed a standard diet or an egg-white diet. Cytokine responses to OVA by PP cells were analyzed as a function of the diet received. PP cells of Bif mice fed a standard diet showed lower levels of IFN-γ and IL-6 production in response to OVA than those of Cont mice. In addition, when mice were fed an egg-white diet, PP cells of Bif mice showed lower levels of IFN-γ and IL-6 production than those of Cont mice. These patterns of cytokine production of Bif mice were similar to those of conventional mice compared with germ-free mice. The results suggest that the association of Bp downregulated excessive cytokine production by PP cells in response to dietary antigen and might contribute to induce the development of normal intestinal immune responses.

肠道中肠道细菌的关联被认为有助于肠道免疫系统的发育和维持稳态。预期功能性肠道微单细菌或益生菌会调节免疫反应或调节宿主中的过敏反应。但是，尚未阐明肠道细菌在肠道免疫系统上的免疫功能。我们通过建立卵巢蛋白（OVA）特异性T细胞受体转基因（OVA-TG）小鼠的Gnotobiotic模型来检查肠道细菌对饮食抗原的肠道免疫抑制反应的直接影响。使用这些小鼠，我们检查了peyer斑块（PP）的双歧杆菌杆菌7041（BP）对免疫调查的影响，Peyer斑块（PP）是肠道免疫系统的电感部位。无菌OVA-TG小鼠与（BIF）或没有（续）BP相关，然后与分割的丝状细菌和梭状芽胞杆菌相关。这些小鼠被喂养标准饮食或卵白饮食。分析了PP细胞对OVA的细胞因子反应，这是收到的饮食的函数。喂养标准饮食的BIF小鼠的PP细胞显示，与cont小鼠相比，对OVA的IFN-γ和IL-6产生水平较低。另外，当小鼠被喂食卵白饮食时，BIF小鼠的PP细胞显示出比CONT小鼠的IFN-γ和IL-6产生水平低。与无菌小鼠相比，这些BIF小鼠细胞因子产生的模式与常规小鼠相似。结果表明，BP的关联下调了PP细胞对饮食抗原的过量细胞因子产生，并可能有助于诱导正常的肠道免疫反应的发展。

项目成果

Senescence-associated decline of lymphocyte migration in gut-associated lymphoid tissues of rat small intestine.

大鼠小肠肠道相关淋巴组织中淋巴细胞迁移的衰老相关下降。

• 发表时间: 2004
• 期刊: Mechanisms of Aging and Development 125(3)
• 作者: Ogino T;Miura S;Komoto S;Hara Y;Hokari R;Tsuzuki Y;Watanabe C;Koseki S;Nagata H;Hachimura S;kaminogawa S;Ishii H
• 通讯作者: Ishii H

Establishment and analysis of germfree T cell receptor transgenic mice.

无菌T细胞受体转基因小鼠的建立与分析。

• 发表时间: 2004
• 期刊: Animal Cell Technology : Basic & Applied Aspects 13
• 作者: Fujioka M;Hachimura S;Hosono A;Nakamura R;Hirayama K;Itoh K;Kaminogawa S
• 通讯作者: Kaminogawa S

Vaccination with an immunodominant peptide of bovine induces type II collagen induces an anti-TCR response, and modulates the onset and severity of collagen-induced arthritis.

使用牛免疫显性肽进行疫苗接种可诱导 II 型胶原蛋白诱导抗 TCR 反应，并调节胶原蛋白诱导的关节炎的发作和严重程度。

• 发表时间: 2004
• 期刊: International Immunology 16(5)
• 作者: Honda A;Ametani A;Matsumoto T;Iwaya A;Kano H;Hachimura S;Ohkawa K;Kaminogawa S;Suzuki K;Sercarz EE;Kumar V
• 通讯作者: Kumar V

Establishment and analysis of germfree T cell receptor trausgenic mice.

无菌T细胞受体致伤小鼠的建立及分析。

• 发表时间: 2004
• 期刊: Animal Cell Technology : Basic & Applied Aspects 13
• 作者: Fujioka M;Hachimura S;Hosono A;Nakamura R;Hirayama K;Itoh K;Kaminogawa S
• 通讯作者: Kaminogawa S

CD4(-)c-kit(-)CD3 epsilon-IL-2R alpha(+) Peyer's patch cells are novel cell subset which secrete IL-5 in response to IL-2 : implications for their role in IgA production.

CD4(-)c-kit(-)CD3 epsilon-IL-2R α( ) 派尔氏斑细胞是响应 IL-2 分泌 IL-5 的新型细胞亚群：对其在 IgA 产生中的作用的影响。

• 发表时间: 2004
• 期刊: European Journal of Immunology 34(7)
• 作者: Kuraoka M;Hashiguchi M;Hachimura S;Kaminogawa S
• 通讯作者: Kaminogawa S